Protein Alignment SPAST and SAP1

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: NP_010966.1
• Gene: SAP1 / 856771
• SGDID: S000000849
• Length: 897
• Species: Saccharomyces cerevisiae
• Alignment Length: 599
• Identity: 209/599 - (34%)
• Similarity: 292/599 - (48%)
• Gaps: 121/599 - (20%)

Human    98 APASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQ-KEQAVEWYKKGIEELEKGIA 161
            |||..|.|.|.                     .|.:|.....|. |..::|
Yeast   336 APALPSLPPPP---------------------LLNVDRASSTGALKPHSLE-------------- 365

Human   162 VIVTGQGEQCERARRLQ-AKMMTN-----LVMAKDRLQLLEKMQPV-LPFSKSQT------DVYN 213
               |.......:.|..| :|:|.|     |...|....|:.|..|. :..|||.|      :..:
Yeast   366 ---TSTTMDSSKIRNPQISKLMKNNHVPYLKGTKSTPTLITKSTPTFITRSKSNTKPIIKSNASS 427

Human   214 DSTNLACRNGHLQSESGAVPKRKDPL-------------THTSNSLPRSKT--VMKTGSAGLSGH 263
            .:::|...|..:|....|....|..|             |..|:.:.:|||  |..:.|...|.|
Yeast   428 PTSSLTVPNSVIQKPKTAAMAAKRVLNSKKVASNPALNTTKKSHPILKSKTAKVPNSSSKKTSSH 492

Human   264 HRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNR-----------------TNKPSTPTTATRKK 311
            ...|..:......|..|...|:........||||                 |...:...|:..::
Yeast   493 PSRPVSNSKPYSHGASQNKKPSKNQTTSMSKTNRKIPAQKKIGSPKIEDVGTEDATEHATSLNEQ 557

Human   312 KD-----------------LKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVI 359
            ::                 :.:.:.||...|..|..|||.:|..|.:|||||.:.||.:|:|.|:
Yeast   558 REEPEIDKKVLREILEDEIIDSLQGVDRQAAKQIFAEIVVHGDEVHWDDIAGLESAKYSLKEAVV 622

Human   360 LPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVR 424
            .|.|||:||.|||.|.||:|||||||.||||||:|||.||::|||:|||:||||||:||.|||||
Yeast   623 YPFLRPDLFRGLREPVRGMLLFGPPGTGKTMLARAVATESHSTFFSISASSLTSKYLGESEKLVR 687

Human   425 ALFAVARELQPSIIFIDEVDSLLCER-REGEHDASRRLKTEFLIEFDGVQSAG------------ 476
            ||||:|::|.|||||:||:||::..| .|.|:::|||:|.|||:::..:.||.
Yeast   688 ALFAIAKKLSPSIIFVDEIDSIMGSRNNENENESSRRIKNEFLVQWSSLSSAAAGSNKSNTNNSD 752

Human   477 ------DDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELA 535
                  |.||||:.|||.|..:|||..|||::|.|:.||.::||.:..|.||..|...||:.:..
Yeast   753 TNGDEDDTRVLVLAATNLPWSIDEAARRRFVRRQYIPLPEDQTRHVQFKKLLSHQKHTLTESDFD 817

Human   536 QLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQT 600
            :|.::|:||||||:|:||||||:||:|:| .:::.......:|.|.|.||..||..||.|||...
Yeast   818 ELVKITEGYSGSDITSLAKDAAMGPLRDL-GDKLLETEREMIRPIGLVDFKNSLVYIKPSVSQDG 881

Human   601 LEAYIRWNKDFGDT 614
            |..|.:|...||.:
Yeast   882 LVKYEKWASQFGSS 895

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		50/247 (20%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194		19/102 (19%)
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196		14/90 (16%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200		15/92 (16%)
		MIT_spastin	116..195	CDD:239142	13/85 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266		13/56 (23%)
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		180/455 (40%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		12/91 (13%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		8/50 (16%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	101/181 (56%)
		AAA_lid_3	533..587	CDD:465537	24/53 (45%)
		Vps4_C	<580..612	CDD:462762	14/31 (45%)
SAP1	NP_010966.1	Herpes_BLLF1	<353..543	CDD:282904	41/206 (20%)
		McrB	<539..>659	CDD:441011	47/119 (39%)
		RecA-like_VPS4-like	607..788	CDD:410917	100/180 (56%)
		AAA_lid_3	815..850	CDD:465537	19/35 (54%)
		Vps4_C	<847..893	CDD:462762	15/45 (33%)