Protein Alignment SPAST and spast

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: XP_012818464.1
• Gene: spast / 549207
• XenbaseID: XB-GENE-947839
• Length: 603
• Species: Xenopus tropicalis
• Alignment Length: 620
• Identity: 452/620 - (72%)
• Similarity: 505/620 - (81%)
• Gaps: 21/620 - (3%)

Human     1 MNSPGGRGKKK---GSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFA 62
            |||||||..||   .....:.|.||.||||   ||......|||  |.||||||.|||||...|:
 Frog     1 MNSPGGRNNKKKPVTPAAETGPGPPTPPPP---PAETQVLLAPP--SLHKRNLYLFSYPLLAAFS 60

Human    63 LLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYI 127
            |||.:||.|||||||.|:|.||.:||.|.|:.|    .:|:|||.....|.|.||.:|:|||:||
 Frog    61 LLRFLAFQLGLLFVWFCERLSRRVMADKGSTAA----RTAAAPAQDRPQEPEVVRSYHQQAFQYI 121

Human   128 SIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRL 192
            |:||||||:|| .|||||::|||||||||||||||.:||:|||.:|||||||||.|||:||||||
 Frog   122 SMALRIDEEEK-DQKEQAIQWYKKGIEELEKGIAVTITGKGEQYDRARRLQAKMSTNLLMAKDRL 185

Human   193 QLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGS 257
            |||.|::..:....||.:|.:|:|||.||||.|:.|.|||||:|||.:.:|||..|.|...|:||
 Frog   186 QLLAKLKADIQGQHSQMEVCSDNTNLPCRNGLLKPEKGAVPKKKDPPSISSNSYSRVKAAPKSGS 250

Human   258 AGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTN-RTNKPSTPTTATRKKKDLKNFRNVD 321
            .|    :|.|:.:|:|  |..:|....||:......|.| |||||:|||||.| |||:||.||||
 Frog   251 LG----NRIPNCTGVS--SSARQAGPNAPSNRGAAGKNNTRTNKPTTPTTAVR-KKDMKNLRNVD 308

Human   322 SNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGN 386
            |||||||:|||||:|..|||.||||||||||||||||||||:|||||||||||||||||||||||
 Frog   309 SNLANLILNEIVDSGPTVKFADIAGQDLAKQALQEIVILPSIRPELFTGLRAPARGLLLFGPPGN 373

Human   387 GKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERR 451
            |||||||||||||||||||||||||||||||||||||||||:|||||||||||||||||||||||
 Frog   374 GKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFSVARELQPSIIFIDEVDSLLCERR 438

Human   452 EGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLL 516
            |||||||||||||||||||||||.||||||||||||||||||:||||||.|||||||||||||||
 Frog   439 EGEHDASRRLKTEFLIEFDGVQSGGDDRVLVMGATNRPQELDDAVLRRFTKRVYVSLPNEETRLL 503

Human   517 LLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIR 581
            ||||||.|||:||.:|||.||:|:|:||||||:|||||||||||||||||||||||:|||||||:
 Frog   504 LLKNLLSKQGNPLNEKELTQLSRLTEGYSGSDITALAKDAALGPIRELKPEQVKNMAASEMRNIK 568

Human   582 LSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV 616
            .|||..||||||.||||.|||:||||||:||||||
 Frog   569 YSDFLSSLKKIKCSVSPSTLESYIRWNKEFGDTTV 603

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		167/302 (55%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194		121/195 (62%)
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80		46/81 (57%)
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50		25/51 (49%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43		21/44 (48%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11		4/6 (67%)
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87		24/36 (67%)
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67		4/7 (57%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196		64/83 (77%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200		65/85 (76%)
		MIT_spastin	116..195	CDD:239142	61/78 (78%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266		19/41 (46%)
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		313/388 (81%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		33/58 (57%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		16/34 (47%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		1/2 (50%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	157/162 (97%)
		AAA_lid_3	533..587	CDD:465537	45/53 (85%)
		Vps4_C	<580..612	CDD:462762	23/31 (74%)
spast	XP_012818464.1	MIT_spastin	110..188	CDD:239142	61/78 (78%)
		RecA-like_spastin	330..493	CDD:410932	157/162 (97%)
		AAA_lid_3	520..554	CDD:465537	28/33 (85%)
		Vps4_C	<567..599	CDD:462762	23/31 (74%)