DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment SPAST and Kat60

DIOPT Version :9

Sequence 1:	NP_055761.2	Gene:	SPAST / 6683	HGNCID:	11233	Length:	616	Species:	Homo sapiens
Sequence 2:	NP_001262276.1	Gene:	Kat60 / 53566	FlyBaseID:	FBgn0040208	Length:	605	Species:	Drosophila melanogaster

Alignment Length:	414	Identity:	154/414 - (37%)
Similarity:	235/414 - (56%)	Gaps:	35/414 - (8%)

- Green bases have known domain annotations that are detailed below.

Human   231 AVPKRKDPLTHTSNSLPRSKTVMKTGSAG-LSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPK 294
            |.|..:.....|.||.|.|.....|...| .|..:...|.|..:...|.:..:|.|......|..
  Fly   197 AAPNNRRTTPATQNSRPSSTIPQSTARNGPASTRNSRNSTSATAPSGGARTTNGRAGGRKLSTSN 261

Human   295 TNRTNKPST-----------------PTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFD 342
            ||......:                 |..|..:::..:...::::.|.:::..:|:.....|::.
  Fly   262 TNEARDDDSTAAGNNGGAAGDGENGDPQAAQEEERKFQPNNHIEAELVDILERDILQKDPKVRWS 326

Human   343 DIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNIS 407
            |||....||:.|:|.|:||.|.|:.|.|:|.|.:|:|:.||||.||||||||||.|...||||:|
  Fly   327 DIADLHDAKRLLEEAVVLPMLMPDYFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVS 391

Human   408 AASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERR--EGEHDASRRLKTEFLIEFD 470
            :|:|||||.||.||:||.||.:||...||.|||||:|| ||.||  |.||:||||:|:|.|::.|
  Fly   392 SATLTSKYRGESEKMVRLLFEMARFYAPSTIFIDEIDS-LCSRRGSESEHEASRRVKSELLVQMD 455

Human   471 GVQSAGDDR---VLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLK-NLLCKQGSPLTQ 531
            || ..|:::   |:|:.|||.|.::|||:.||..||:|:.||::|.|..||| ||  ::......
  Fly   456 GV-GGGEEQAKVVMVLAATNFPWDIDEALRRRLEKRIYIPLPSDEGREALLKINL--REVKVDDS 517

Human   532 KELAQLARMTDGYSGSDLTALAKDAALGPIRE----LKPEQVKNMSASEMRNIRLS--DFTESLK 590
            .:|..:|....||||:|:|.:.::|::..:|.    |.|||::.::..|: ::.:|  ||.|::.
  Fly   518 VDLTYVANELKGYSGADITNVCREASMMSMRRKIAGLTPEQIRQLATEEV-DLPVSNKDFNEAMS 581

Human   591 KIKRSVSPQTLEAYIRWNKDFGDT 614
            :..:|||...|:.|.:|.::||.:
  Fly   582 RCNKSVSRADLDKYEKWMREFGSS 605

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269\|PubMed:16602018	1..300		18/69 (26%)
		Required for midbody localization. /evidence=ECO:0000269\|PubMed:18997780	1..194
		Required for interaction with ATL1. /evidence=ECO:0000269\|PubMed:16339213, ECO:0000269\|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269\|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269\|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269\|PubMed:18997780	112..196
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:15269182	114..200
		MIT_spastin	116..195	CDD:239142
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	224..266		10/35 (29%)
		Sufficient for microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	228..616		154/414 (37%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	270..328		10/74 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	278..312		7/50 (14%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	309..312		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	94/167 (56%)
		AAA_lid_3	533..587	CDD:407720	18/59 (31%)
		Vps4_C	<580..612	CDD:401324	10/33 (30%)
Kat60	NP_001262276.1	HCV_NS5a_C	<163..243	CDD:289693	12/45 (27%)
		P-loop_NTPase	325..>381	CDD:304359	31/55 (56%)
		AAA	358..496	CDD:214640	80/139 (58%)
		AAA	362..495	CDD:278434	78/134 (58%)
		Vps4_C	<570..603	CDD:286426	10/32 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910