DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment SPAST and Spast

DIOPT Version :9

Sequence 1:	NP_055761.2	Gene:	SPAST / 6683	HGNCID:	11233	Length:	616	Species:	Homo sapiens
Sequence 2:	NP_001156342.1	Gene:	Spast / 50850	MGIID:	1858896	Length:	614	Species:	Mus musculus

Alignment Length:	616	Identity:	570/616 - (92%)
Similarity:	579/616 - (93%)	Gaps:	2/616 - (0%)

- Green bases have known domain annotations that are detailed below.

Human     1 MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLR 65
            |:||.||.||||||||| |.|.|||||...|| |||||||...||.|||...||.||.|||||||
Mouse     1 MSSPAGRRKKKGSGGAS-PAPARPPPPAAVPA-PAAGPAPAAGSPPKRNPSSFSSPLVVGFALLR 63

Human    66 LVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIA 130
            |:|.||||||.||||||||||||||||||.||||||.|.|.|.||||||.|||||||||||||||
Mouse    64 LLACHLGLLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIA 128

Human   131 LRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLL 195
            |||||:||||||||||||||||||||||||||||||||||.||||||||||||||||||||||||
Mouse   129 LRIDEEEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLL 193

Human   196 EKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGL 260
            ||:||||.||||||||||:||||.||||||||||||||||||||||.||||||||||:|:|||||
Mouse   194 EKLQPVLQFSKSQTDVYNESTNLTCRNGHLQSESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGL 258

Human   261 SGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLA 325
            ||||||||.||||||||.:.|.|||.||||||||.||||||||||||.|||||||||||||||||
Mouse   259 SGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLA 323

Human   326 NLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTM 390
            ||||||||||||||||||||||:||||||||||||||||||||||||||||||||||||||||||
Mouse   324 NLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTM 388

Human   391 LAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEH 455
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Mouse   389 LAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEH 453

Human   456 DASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKN 520
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Mouse   454 DASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKN 518

Human   521 LLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDF 585
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Mouse   519 LLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDF 583

Human   586 TESLKKIKRSVSPQTLEAYIRWNKDFGDTTV 616
            |||||||||||||||||||||||||||||||
Mouse   584 TESLKKIKRSVSPQTLEAYIRWNKDFGDTTV 614

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269\|PubMed:16602018	1..300		254/298 (85%)
		Required for midbody localization. /evidence=ECO:0000269\|PubMed:18997780	1..194		162/192 (84%)
		Required for interaction with ATL1. /evidence=ECO:0000269\|PubMed:16339213, ECO:0000269\|PubMed:16815977	1..80		56/78 (72%)
		Required for nuclear localization. /evidence=ECO:0000269\|PubMed:15147984	1..50		34/48 (71%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..43		30/41 (73%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	4..11		4/6 (67%)
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269\|PubMed:15269182	50..87		28/36 (78%)
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:16026783	59..67		7/7 (100%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269\|PubMed:18997780	112..196		80/83 (96%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:15269182	114..200		81/85 (95%)
		MIT_spastin	116..195	CDD:239142	76/78 (97%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	224..266		38/41 (93%)
		Sufficient for microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	228..616		375/387 (97%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	270..328		50/57 (88%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	278..312		26/33 (79%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	309..312		2/2 (100%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:23272056	310..312		1/1 (100%)
		RecA-like_spastin	343..506	CDD:410932	161/162 (99%)
		AAA_lid_3	533..587	CDD:407720	53/53 (100%)
		Vps4_C	<580..612	CDD:401324	31/31 (100%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..298		254/298 (85%)
		Required for midbody localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..192		162/192 (84%)
		Required for interaction with ATL1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..78		56/78 (72%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..48		34/48 (71%)
		Required for nuclear localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..48		34/48 (71%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	4..11		4/6 (67%)
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	48..85		28/36 (78%)
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	57..65		7/7 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	91..112		15/20 (75%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	110..194		80/83 (96%)
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	112..198		81/85 (95%)
		MIT_spastin	114..193	CDD:239142	76/78 (97%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	220..306		75/85 (88%)
		Sufficient for microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	226..614		375/387 (97%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	268..326		50/57 (88%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	307..310		2/2 (100%)
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	308..310		1/1 (100%)
		P-loop_NTPase	339..>395	CDD:304359	54/55 (98%)
		AAA	372..508	CDD:214640	135/135 (100%)
		AAA	376..506	CDD:278434	129/129 (100%)
		Vps4_C	<578..610	CDD:286426	31/31 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C83955738
Domainoid	1	1.000	643	1.000	Domainoid score	I4512
eggNOG	0	0.000	Not matched by this tool.
HGNC	1	1.500	-	-
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H8970
Inparanoid	1	1.050	1099	1.000	Inparanoid score	I5037
Isobase	1	0.950	-	0	Normalized mean entropy	S1340
NCBI	1	1.000	-	-
OMA	1	1.010	-	-		QHG48730
OrthoDB	1	1.010	-	-		D1176820at2759
OrthoFinder	1	1.000	-	-		FOG0001088
OrthoInspector	1	1.000	-	-		oto125260
orthoMCL	1	0.900	-	-		OOG6_100722
Panther	1	1.100	-	-	LDO	PTHR23074
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2807
SonicParanoid	1	1.000	-	-		X3342
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	18	18.350