DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment SPAST and spas

DIOPT Version :9

Sequence 1:	NP_055761.2	Gene:	SPAST / 6683	HGNCID:	11233	Length:	616	Species:	Homo sapiens
Sequence 2:	NP_651206.3	Gene:	spas / 42846	FlyBaseID:	FBgn0039141	Length:	758	Species:	Drosophila melanogaster

Alignment Length:	729	Identity:	320/729 - (43%)
Similarity:	404/729 - (55%)	Gaps:	144/729 - (19%)

- Green bases have known domain annotations that are detailed below.

Human     6 GRGKKKGSGGASNPVPPRPPP---------PCLAP--APPAAGP----APPPESPHKRNLYYFSY 55
            |.....|.|.:||...|...|         ..|.|  ..|.:|.    .....|.||:|||..|:
  Fly    56 GGSSAAGGGSSSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSF 120

Human    56 PLFVGFALLRLVAFHLGLLFVWL---------------C-------------------------- 79
            |:...|.:||.:.:.|..:|.:|               |
  Fly   121 PIIFLFNVLRSLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVVQNSSKEQQQSLNHPSELNRE 185

Human    80 ------------QRFSRAL----MAAKRSSGAAPAPASASAPAPVPGGE-AERVRVFHKQAFEYI 127
                        ||| |.:    |||.|..|..         :|.||.. ..:.:..|::|||||
  Fly   186 GDGQEQQLSNQPQRF-RPIQPLEMAANRPGGGY---------SPGPGDPLLAKQKHHHRRAFEYI 240

Human   128 SIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAV-IVTGQGEQCERARRLQAKMMTNLVMAKDR 191
            |.||:||| |..|.||.|:|.|:|||:|||.|||| ..:|:|:..:||:||..||.|||.||:||
  Fly   241 SKALKIDE-ENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDR 304

Human   192 LQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTG 256
            |..|...:..|...:           |:.:....:.......|.::|:.....:.|....|..:|
  Fly   305 LHFLALREQDLQMQR-----------LSLKEKQKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSG 358

Human   257 SAGLSGHHRAPSYSGLSMVSGVKQ-----------------GS---------GPAPTT------- 288
            ....:.....|:.||..:..|.|:                 ||         .||.|.
  Fly   359 YGPKATTSAQPTASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVR 423

Human   289 ------HKGTPKTNRT----NKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDD 343
                  ....|:.:||    |.||....:|    .:.:.:.|:..|..||::|||:.|..|::.|
  Fly   424 RQFSSGRNTPPQRSRTPINNNGPSGSGAST----PVVSVKGVEQKLVQLILDEIVEGGAKVEWTD 484

Human   344 IAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISA 408
            |||||:|||||||:|||||:||||||||||||:|||||||||||||:||:|||.|.:|||.||||
  Fly   485 IAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISA 549

Human   409 ASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQ 473
            |||||||||:|||||||||||||.:||||||||||||||.||...||:||||||||||:||||:.
  Fly   550 ASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLP 614

Human   474 SAGD-DRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQL 537
            ...| ||::|:.|||||||||||.||||.||||||||:|:||.|||..||.||||||..:.|.:|
  Fly   615 GNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRL 679

Human   538 ARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLE 602
            |::||||||||||||||||||.|||||..||||.:..|.||.|...||..|||:|:|||:||:|.
  Fly   680 AKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLN 744

Human   603 AYIRWNKDFGDTTV 616
            :|.:|::|:||.|:
  Fly   745 SYEKWSQDYGDITI 758

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269\|PubMed:16602018	1..300		104/410 (25%)
		Required for midbody localization. /evidence=ECO:0000269\|PubMed:18997780	1..194		83/261 (32%)
		Required for interaction with ATL1. /evidence=ECO:0000269\|PubMed:16339213, ECO:0000269\|PubMed:16815977	1..80		26/141 (18%)
		Required for nuclear localization. /evidence=ECO:0000269\|PubMed:15147984	1..50		14/58 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..43		11/51 (22%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	4..11		1/4 (25%)
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269\|PubMed:15269182	50..87		15/93 (16%)
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:16026783	59..67		3/7 (43%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269\|PubMed:18997780	112..196		45/85 (53%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:15269182	114..200		46/86 (53%)
		MIT_spastin	116..195	CDD:239142	45/79 (57%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	224..266		5/41 (12%)
		Sufficient for microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	228..616		233/431 (54%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	270..328		18/100 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	278..312		13/76 (17%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	309..312		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	132/163 (81%)
		AAA_lid_3	533..587	CDD:407720	36/53 (68%)
		Vps4_C	<580..612	CDD:401324	16/31 (52%)
spas	NP_651206.3	MIT_spastin	230..308	CDD:239142	45/78 (58%)
		P-loop_NTPase	482..>538	CDD:304359	47/55 (85%)
		AAA	515..652	CDD:214640	108/136 (79%)
		AAA	519..650	CDD:278434	103/130 (79%)
		Vps4_C	<722..754	CDD:286426	16/31 (52%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165146778
Domainoid	1	1.000	201	1.000	Domainoid score	I3005
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H8970
Inparanoid	1	1.050	480	1.000	Inparanoid score	I1477
Isobase	1	0.950	-	0	Normalized mean entropy	S1340
OMA	1	1.010	-	-		QHG48730
OrthoDB	1	1.010	-	-		D1176820at2759
OrthoFinder	1	1.000	-	-		FOG0001088
OrthoInspector	1	1.000	-	-		oto90979
orthoMCL	1	0.900	-	-		OOG6_100722
Panther	1	1.100	-	-	LDO	PTHR23074
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2807
SonicParanoid	1	1.000	-	-		X3342
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.850