Protein Alignment SPAST and Nsf2

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: NP_788676.1
• Gene: Nsf2 / 41694
• FlyBaseID: FBgn0266464
• Length: 752
• Species: Drosophila melanogaster
• Alignment Length: 352
• Identity: 101/352 - (28%)
• Similarity: 164/352 - (46%)
• Gaps: 75/352 - (21%)

Human   294 KTNRTNKPSTPTTATRKKKDLK----------NFRNVDSNLANL------------IMNEIVDNG 336
            ||.....|.|...:..|.::::          .|...::::.||            |:|...|.|
  Fly   157 KTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKAENSVLNLQGRSKGKIVRQSIINPDWDFG 221

Human   337 TAVKFDDIAGQD-----LAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVA 396
            ..    .|.|.|     :.::|....|..|.|..:|  |:: ..:|:||:||||.|||::|:.:.
  Fly   222 KM----GIGGLDKEFNAIFRRAFASRVFPPELVEQL--GIK-HVKGILLYGPPGTGKTLMARQIG 279

Human   397 AESNATFFNI-SAASLTSKYVGEGEKLVRALFAVARE----LQPS----IIFIDEVDSLLCERR- 451
            ...||....| :...:..|||||.|..:|.|||.|.|    |.|:    ||..||:|: :|:.|
  Fly   280 TMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKRLGPNSGLHIIIFDEIDA-ICKARG 343

Human   452 -----EGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLR--RFIKRVYVSLP 509
                 .|.||.   :..:.|.:.|||:..  :.:||:|.|||...:|||:||  |...::.:|||
  Fly   344 SVAGNSGVHDT---VVNQLLAKIDGVEQL--NNILVIGMTNRRDMIDEALLRPGRLEVQMEISLP 403

Human   510 NEETRLLLLKNLLCKQ-------GSPLTQKELAQLARMTDGYSGSDLTAL---AKDAALGPIREL 564
            ||:.|:.:| |:..|:       .|.:...|   :|..|..:||::|..|   |:..|:.  |.:
  Fly   404 NEQGRVQIL-NIHTKRMRDFNKIASDVDNNE---IAAKTKNFSGAELEGLVRAAQSTAMN--RLI 462

Human   565 KPEQVKNMSASEMRNIRL--SDFTESL 589
            |.:...::....|..:|:  :||..:|
  Fly   463 KADSKVHVDPEAMEKLRVTRADFLHAL 489

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		2/5 (40%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200
		MIT_spastin	116..195	CDD:239142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		101/352 (29%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		8/55 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		5/17 (29%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		1/2 (50%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		1/1 (100%)
		RecA-like_spastin	343..506	CDD:410932	63/184 (34%)
		AAA_lid_3	533..587	CDD:465537	15/58 (26%)
		Vps4_C	<580..612	CDD:462762	4/12 (33%)
Nsf2	NP_788676.1	CDC48_N	9..89	CDD:215012
		CDC48_2	117..>170	CDD:215011	4/12 (33%)
		RecA-like_NSF-SEC18_r1-like	224..400	CDD:410912	63/184 (34%)
		CDC48	<225..>611	CDD:273521	89/280 (32%)