Protein Alignment SPAST and spast

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: NP_998080.2
• Gene: spast / 405851
• ZFINID: ZDB-GENE-040426-2331
• Length: 570
• Species: Danio rerio
• Alignment Length: 589
• Identity: 374/589 - (63%)
• Similarity: 441/589 - (74%)
• Gaps: 42/589 - (7%)

Human    35 AAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAP 99
            |.||.....:...|.|:|......|...|||:    |.||..||.|...||:.|..:..|
Zfish    14 ACGPVSDGSARGNRLLFYTRSLSRVPEWLLRV----LLLLLRWLFQPIRRAMAARAKECG----- 69

Human   100 ASASAPAPVPGGE---AERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIA 161
                     |.|.   .||:|.:||||||:||:||:|||||| |.|::||:||:|||.||||||.
Zfish    70 ---------PDGSEETGERIRNYHKQAFEFISVALQIDEDEK-GDKQKAVQWYRKGIAELEKGIQ 124

Human   162 VIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQ 226
            :.|||.||:.:|||:||.||:|||.||:|||:||..:     .|:|..:..:|.:..:..||:|:
Zfish   125 IQVTGAGEKADRARKLQDKMITNLSMAEDRLKLLGNL-----LSQSPAESSSDDSFYSFSNGNLR 184

Human   227 --SESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTH 289
              ..||||.|:||.||.|:.:..|.|...|:          .|:.|||:......|.|...|..:
Zfish   185 PAPASGAVSKKKDTLTITNQTSLRPKNPPKS----------TPNASGLNCTPSAAQSSRTGPQNN 239

Human   290 KGTPKTNRTNKPSTPTTAT---RKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAK 351
            :..|.....|.....||||   ::|:|:|||:||||.||:||:|||||:|:.|:|||||||||||
Zfish   240 QKGPTVKGKNNVKASTTATASPQRKRDMKNFKNVDSKLASLILNEIVDSGSVVRFDDIAGQDLAK 304

Human   352 QALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYV 416
            ||||||||||:||||||||||||||||||||||||||||||||||.||||||||||||:||||||
Zfish   305 QALQEIVILPALRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAMESNATFFNISAATLTSKYV 369

Human   417 GEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVL 481
            ||||||||||||||||||||||||||:|||||||||||||||||||||||||||||||.||:|||
Zfish   370 GEGEKLVRALFAVARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIEFDGVQSGGDERVL 434

Human   482 VMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSG 546
            |||||||||||||||||||.||:||:||.|||||.||||||.|..:||:||||:||||:||||||
Zfish   435 VMGATNRPQELDEAVLRRFAKRIYVALPTEETRLKLLKNLLSKHRNPLSQKELSQLARLTDGYSG 499

Human   547 SDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDF 611
            ||||:||||||||||||||||||:||||.|||:||:|||.||||:||||||||||:.|:|||:::
Zfish   500 SDLTSLAKDAALGPIRELKPEQVRNMSAHEMRDIRISDFLESLKRIKRSVSPQTLDQYVRWNREY 564

Human   612 GDTT 615
            ||||
Zfish   565 GDTT 568

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		105/269 (39%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194		76/161 (47%)
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80		15/44 (34%)
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50		4/14 (29%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43		3/7 (43%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87		14/36 (39%)
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67		4/7 (57%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196		55/86 (64%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200		56/85 (66%)
		MIT_spastin	116..195	CDD:239142	52/78 (67%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266		14/43 (33%)
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		290/391 (74%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		23/60 (38%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		9/36 (25%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	154/162 (95%)
		AAA_lid_3	533..587	CDD:465537	46/53 (87%)
		Vps4_C	<580..612	CDD:462762	23/31 (74%)
spast	NP_998080.2	MIT_spastin	80..158	CDD:239142	52/78 (67%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	186..269		28/92 (30%)
		RecA-like_spastin	296..459	CDD:410932	154/162 (95%)
		AAA_lid_3	486..534	CDD:465537	41/47 (87%)
		Vps4_C	<532..565	CDD:462762	23/32 (72%)