Protein Alignment SPAST and TER94

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: NP_001097249.1
• Gene: TER94 / 36040
• FlyBaseID: FBgn0286784
• Length: 826
• Species: Drosophila melanogaster
• Alignment Length: 549
• Identity: 148/549 - (26%)
• Similarity: 237/549 - (43%)
• Gaps: 132/549 - (24%)

Human   139 AGQKEQAVEWYKKGIEELEKGIAVIV---------------TGQGEQCERARRLQAKMMTNLVMA 188
            ||:.|..:   :|..||.||....|:               .|:.|     ||:.::::|
  Fly   301 AGESESNL---RKAFEEAEKNSPAIIFIDEIDAIAPKRDKTHGEVE-----RRIVSQLLT----- 352

Human   189 KDRLQLLEKMQPVLPFSKSQTDVYNDSTN-------LACRNGHLQSESG-AVPKRKDPL----TH 241
                 |::.|:      ||...:...:||       ...|.|....|.. .:|.....|    .|
  Fly   353 -----LMDGMK------KSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRIH 406

Human   242 TSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTT 306
            |.|........::..:|...||..| ..:.|...:.::|
  Fly   407 TKNMKLHDDVDLEQIAAESHGHVGA-DLASLCSEAALQQ-------------------------- 444

Human   307 ATRKKKDL--------------------KNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAK 351
             .|:|.||                    :|||...:..:...:.|.|.......:.||.|.:..|
  Fly   445 -IREKMDLIDLEDDKIDAEVLASLAVTMENFRYAMTKSSPSALRETVVEVPNTTWTDIGGLESVK 508

Human   352 QALQEIVILPSLRPELFT--GLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSK 414
            :.|||:|..|...|:.|.  |:: |:||:|.:||||.|||:||||:|.|..|.|.::....|.:.
  Fly   509 KELQELVQYPVEHPDKFLKFGMQ-PSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTM 572

Human   415 YVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHD---ASRRLKTEFLIEFDGVQSAG 476
            :.||.|..||.:|..||...|.::|.||:||:...|.....|   |:.|:..:.|.|.||:  ..
  Fly   573 WFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGM--GA 635

Human   477 DDRVLVMGATNRPQELDEAVLR--RFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQK-ELAQLA 538
            ...|.::||||||..:|.|:||  |..:.:|:.||::::|..:||..|.|  |||.:: :|..:|
  Fly   636 KKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRK--SPLAKEVDLTYIA 698

Human   539 RMTDGYSGSDLTALAKDAALGPIREL-------KPEQVKNM-SASEM------RNIRLSDFTESL 589
            ::|.|:||:|||.:.:.|....||:.       :.|:.:|. ||.:|      ..|..:.|.|::
  Fly   699 KVTQGFSGADLTEICQRACKLAIRQAIEAEIRREKERAENQNSAMDMDEDDPVPEITSAHFEEAM 763

Human   590 KKIKRSVSPQTLEAY------IRWNKDFG 612
            |..:||||...:..|      ::.::.||
  Fly   764 KFARRSVSDNDIRKYEMFAQTLQQSRGFG 792

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		34/187 (18%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194		14/69 (20%)
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196		15/71 (21%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200		16/75 (21%)
		MIT_spastin	116..195	CDD:239142	14/70 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266		9/46 (20%)
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		126/438 (29%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		9/77 (12%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		2/33 (6%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		1/2 (50%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	66/169 (39%)
		AAA_lid_3	533..587	CDD:465537	19/67 (28%)
		Vps4_C	<580..612	CDD:462762	9/37 (24%)
TER94	NP_001097249.1	CDC48	47..787	CDD:273521	146/542 (27%)