Protein Alignment SPAST and CG4701

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: NP_609721.1
• Gene: CG4701 / 34858
• FlyBaseID: FBgn0028868
• Length: 384
• Species: Drosophila melanogaster
• Alignment Length: 310
• Identity: 117/310 - (37%)
• Similarity: 183/310 - (59%)
• Gaps: 36/310 - (11%)

Human   315 KNFRNVDSN------LANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTG--- 370
            |.||..|.|      .::|:..|.:|    :.:.||||.|...|.|:|.|:||....:||:.
  Fly    66 KKFRARDFNEHEMMIASHLVTPEDID----ISWSDIAGLDGTIQELRETVVLPVRHRKLFSRSKL 126

Human   371 LRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQP 435
            .||| :|:||.||||.|||::|||:|.::...|.|:....||.|:.||.:||..|:|.:|::|||
  Fly   127 WRAP-KGVLLHGPPGCGKTLIAKAIAKDAGMRFINLDVGVLTDKWYGESQKLATAVFTLAKKLQP 190

Human   436 SIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRF 500
            .||||||::|.|..|...:|:|:..:||:|::::||:.|..:..|||:|||||||:||:|:|||.
  Fly   191 CIIFIDEIESFLRMRGSNDHEATAMIKTQFMLQWDGLMSNTNICVLVLGATNRPQDLDKAILRRM 255

Human   501 IKRVYVSLPNEETRLLLLKNLL-CKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIREL 564
            ..:.::.:|.:..|..:|:.:| .:|.||  ...|.:|||:|.|:|||||..|.:.|::..:|:.
  Fly   256 PAQFHIGVPRDCQRREILQLILQTEQLSP--SVNLKELARLTIGFSGSDLRELCRHASMYRMRQF 318

Human   565 KPE-----------------QVKNMSASEMRN--IRLSDFTESLKKIKRS 595
            ..|                 :||:.:..|..:  |::.|..:||..:|.|
  Fly   319 MREKLNTGEEIGKDKIEWDFEVKDQALQEWEHLEIQMEDLLKSLSVMKAS 368

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200
		MIT_spastin	116..195	CDD:239142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		117/310 (38%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		5/18 (28%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312
		RecA-like_spastin	343..506	CDD:410932	78/165 (47%)
		AAA_lid_3	533..587	CDD:465537	20/72 (28%)
		Vps4_C	<580..612	CDD:462762	6/16 (38%)
CG4701	NP_609721.1	CDC48	<21..368	CDD:273521	116/308 (38%)
		RecA-like_ATAD1	96..261	CDD:410928	78/165 (47%)