DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment SPAST and CG5776

DIOPT Version :9

Sequence 1:	NP_055761.2	Gene:	SPAST / 6683	HGNCID:	11233	Length:	616	Species:	Homo sapiens
Sequence 2:	NP_001260420.1	Gene:	CG5776 / 34680	FlyBaseID:	FBgn0032450	Length:	799	Species:	Drosophila melanogaster

Alignment Length:	628	Identity:	155/628 - (24%)
Similarity:	266/628 - (42%)	Gaps:	171/628 - (27%)

- Green bases have known domain annotations that are detailed below.

Human   117 RVFHKQAFEYI-----SIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAV-------------- 162
            |||:|.:..::     |:..|:             |.::..:||...|:::
  Fly   196 RVFYKDSLIHMNFFNKSLTFRL-------------ERWQSTVEEALAGLSLDSKPLQFIQVTNVT 247

Human   163 ---IVTGQGEQCERARRLQAKMMTNLVMAKDR-LQLLEK-MQPVLPF------------------ 204
               ::|....|.|..:::..::....:...|| |||:|: |:..|.|
  Fly   248 KLHLITDDANQQEEEQKISHRITKCQIGGLDRQLQLVEESMEYALGFRTLPAGLRVSRGLLLYGA 312

Human   205 -----------------SKSQTDV----YNDSTNLACRNGHLQSESGAVPKR-----KDP----L 239
                             .:||..|    .|.....:...|..:.:.||:.:|     ..|    :
  Fly   313 TGCGKSMVLEAMCAVAEERSQGHVQLIRINSGEVYSKFLGETEQKLGAIFERAYNHYPHPTLLLI 377

Human   240 THTSNSLPR---SKTVMKTGSAGLSGHHRAPSYSGLSMVSGVK----QGSGPAPTTHKGTPKTNR 297
            ....|..|:   |..|.:...|.||   .....|..|.:.|.|    ..|....|.|....:..|
  Fly   378 EDVHNLCPKQENSDLVKRVSLAFLS---LLDQLSSPSQLKGSKTFVLATSSQIDTLHPSIRRAGR 439

Human   298 TNKP---STPTTATR---------------KKKDLKNFRN-----VDSNLANLI----------- 328
            .:..   ..|::..|               ..:::::..:     |.::||||:
  Fly   440 LDNEVELGAPSSQARLEIVRCLIKSVEHQLSDEEVEHVASITHGYVGADLANLVYAAMLQAQPNP 504

Human   329 -----------------MNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELF--TGLRAP 374
                             |.|::.....|::.||.||...:.|:|:.:..|.|..:.|  .|:: |
  Fly   505 LQMPHLQAALTRIKPSAMREVLIECPNVQWSDIGGQSELRLAMQQAIEWPLLHADKFQRLGIK-P 568

Human   375 ARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIF 439
            .||:|:|||||..|||:|||:|.||...|.:|....|.|.:|||.|:.||.:|..||::.|:|:|
  Fly   569 PRGILMFGPPGCSKTMIAKALATESKLNFLSIKGPELFSMWVGESERAVREVFRKARQVAPAIVF 633

Human   440 IDEVDSLLCERREGEHDAS-----RRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLR- 498
            .||:|::..||.||:..:|     .|:.|:.|.|.|||::.  ..|.::.|||||..:|:|:||
  Fly   634 FDEIDAIGGERSEGDGSSSGSSVKERVLTQLLTELDGVEAL--QNVTIVAATNRPDMIDKALLRP 696

Human   499 -RFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQK-ELAQLARMTDGYSGSDLTALAKDAALGPI 561
             |..:.:||.||..|.|..:||..|  :..|::.. ::.:|.::|:||||:::.|:..:|||..:
  Fly   697 GRIDRILYVGLPQCEARREILKIKL--RAMPISNDVDMEKLVQLTEGYSGAEIQAVCHEAALRAL 759

Human   562 RELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAY 604
            .:          :.|..:::.:||..:||.:....||:.|:.|
  Fly   760 EQ----------SFEAEDVKWTDFEHALKAVPPRTSPELLKLY 792

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269\|PubMed:16602018	1..300		46/261 (18%)
		Required for midbody localization. /evidence=ECO:0000269\|PubMed:18997780	1..194		16/99 (16%)
		Required for interaction with ATL1. /evidence=ECO:0000269\|PubMed:16339213, ECO:0000269\|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269\|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269\|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269\|PubMed:18997780	112..196		18/101 (18%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:15269182	114..200		20/106 (19%)
		MIT_spastin	116..195	CDD:239142	17/100 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	224..266		11/53 (21%)
		Sufficient for microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	228..616		128/454 (28%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269\|PubMed:15269182	270..328		14/84 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	278..312		7/55 (13%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021, ECO:0000269\|PubMed:15147984	309..312		1/17 (6%)
		Required for interaction with microtubules. /evidence=ECO:0000269\|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	70/171 (41%)
		AAA_lid_3	533..587	CDD:407720	13/53 (25%)
		Vps4_C	<580..612	CDD:401324	8/25 (32%)
CG5776	NP_001260420.1	SpoVK	288..785	CDD:223540	132/514 (26%)
		AAA	307..447	CDD:278434	24/142 (17%)
		AAA	572..707	CDD:278434	59/136 (43%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910