Protein Alignment SPAST and Vps4

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: NP_573258.1
• Gene: Vps4 / 32777
• FlyBaseID: FBgn0283469
• Length: 442
• Species: Drosophila melanogaster
• Alignment Length: 364
• Identity: 141/364 - (38%)
• Similarity: 211/364 - (57%)
• Gaps: 42/364 - (11%)

Human   290 KGTPKTNRTNKPSTPTTATRKKKDLKNFRNVD---SNLANLIMNEIVDNGTAVKFDDIAGQDLAK 351
            ||..|..:....|:......||.|..:....|   ..|.:.:.:.||.....|::.|:||.|.||
  Fly    77 KGKKKPIKEGGESSAKDDKDKKSDSDDEDGDDPEKKKLQSKLEDAIVIEKPKVQWSDVAGLDAAK 141

Human   352 QALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESN-ATFFNISAASLTSKY 415
            :||:|.||||...|:||||.|.|.:|:|||||||.||:.||||||.|:| :|||::|::.|.||:
  Fly   142 EALKEAVILPIKFPQLFTGKRIPWKGILLFGPPGTGKSYLAKAVATEANRSTFFSVSSSDLMSKW 206

Human   416 VGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRV 480
            :||.||||:.||.:||:.:||||||||:||:...|.:.|:|:.||:|||||::..||.: ..|.:
  Fly   207 LGESEKLVKNLFELARQHKPSIIFIDEIDSMCSARSDNENDSVRRIKTEFLVQMQGVGN-DTDGI 270

Human   481 LVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYS 545
            ||:||||.|..||.|:.|||.||:|:.||....||::.|..|......||:::|.:||..|:|||
  Fly   271 LVLGATNIPWVLDSAIRRRFEKRIYIPLPEAHARLVMFKIHLGNTTHVLTEQDLKELAGKTEGYS 335

Human   546 GSDLTALAKDAALGPIRELKP---------------EQVKN----------MSASEMR------- 578
            |:|::.:.:||.:.|:|:::.               |::.|          ..|.||.
  Fly   336 GADISIVVRDALMEPVRKVQTATHFKRVSGPSPTNHEEIVNDLLVPCSPGDQGAVEMNWMDVPSD 400

Human   579 -----NIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFG 612
                 .:.:.|..:||.:.|.:|:...|:...::.:|||
  Fly   401 KLFEPPVTMRDMLKSLSRTKPTVNEDDLKKLRKFTEDFG 439

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		3/9 (33%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200
		MIT_spastin	116..195	CDD:239142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		141/364 (39%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		9/40 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		5/21 (24%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		1/2 (50%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		1/1 (100%)
		RecA-like_spastin	343..506	CDD:410932	93/163 (57%)
		AAA_lid_3	533..587	CDD:465537	19/90 (21%)
		Vps4_C	<580..612	CDD:462762	7/31 (23%)
Vps4	NP_573258.1	MIT_VPS4	6..69	CDD:239141
		RecA-like_VPS4	126..296	CDD:410929	94/170 (55%)
		AAA_lid_3	323..361	CDD:465537	13/37 (35%)
		Vps4_C	379..439	CDD:462762	10/59 (17%)