Protein Alignment SPAST and CG10793

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: NP_570054.2
• Gene: CG10793 / 31308
• FlyBaseID: FBgn0288967
• Length: 479
• Species: Drosophila melanogaster
• Alignment Length: 390
• Identity: 124/390 - (31%)
• Similarity: 195/390 - (50%)
• Gaps: 67/390 - (17%)

Human   278 VKQGSGPAPTTHKGTPKTNRTNKPSTPTT-------------ATR----------KKKDLKNFRN 319
            |:.|.|.|   |:.||......||  |||             ||:          ||.:.....|
  Fly   101 VEMGGGKA---HEKTPAKELPKKP--PTTDASLANWHIGVGAATQALSNEQPLQIKKMETATESN 160

Human   320 VDSN-----------------------LANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILP 361
            ...|                       ||.|:...|:.....:|:.|:.|...|.:.::|.|:.|
  Fly   161 GQDNACEIEHVHLGGDDVLFSSLEWQSLAELVKTSILQENIKIKWSDVCGNQRAIELIKEAVLTP 225

Human   362 SLRPELFT-GLRAPARGLLLFGPPGNGKTMLAKAVAAES--NATFFNISAASLTSKYVGEGEKLV 423
            ...|:||. ||: |.|.|||.||||:|||:||||:.:|:  ..|||||:|:.:.||:.||.||::
  Fly   226 IEFPQLFAHGLK-PWRSLLLHGPPGSGKTLLAKALYSETQGQVTFFNITASIMVSKWRGESEKIL 289

Human   424 RALFAVARELQPSIIFIDEVDSLLCER-REGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATN 487
            |.||.:|.:..||:||.||::||..:| |..:|::|:|.|.|.|...||::.:.:. |.|:.:||
  Fly   290 RVLFHMAAKRAPSVIFFDEIESLTSKRDRATDHESSKRFKNELLQLLDGMEHSLNG-VFVLASTN 353

Human   488 RPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGS--PLTQKELAQLARMTDGYSGSDLT 550
            .|.::|||.||||.|::.|.|||...|..|:..||   ||  .|..:.|.||..::|.::|.::.
  Fly   354 LPWDIDEAFLRRFEKKLLVQLPNAAERSCLINRLL---GSSISLNPRLLEQLVEISDHFTGDEIR 415

Human   551 ALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSP---QTLEAYIRWNKDFG 612
            ...|:.::..:|  ...::.:.|...:.....:....:::|..|.|.|   :.|..:.:|.::.|
  Fly   416 LACKEISMHRVR--CATKIGDRSIGLLAKESPAAIEANVEKAFRQVRPLGQKLLAKHEQWQQENG 478

Human   613  612
              Fly   479  478

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		7/21 (33%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200
		MIT_spastin	116..195	CDD:239142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		124/390 (32%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		20/95 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		15/56 (27%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		1/12 (8%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		1/1 (100%)
		RecA-like_spastin	343..506	CDD:410932	75/166 (45%)
		AAA_lid_3	533..587	CDD:465537	8/53 (15%)
		Vps4_C	<580..612	CDD:462762	6/34 (18%)
CG10793	NP_570054.2	LisH	31..55	CDD:462501
		RecA-like_VPS4-like	208..372	CDD:410917	74/165 (45%)