Protein Alignment SPAST and spas-1

DIOPT Version :10

• Sequence 1: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Sequence 2: NP_001256115.1
• Gene: spas-1 / 179300
• WormBaseID: WBGene00016045
• Length: 512
• Species: Caenorhabditis elegans
• Alignment Length: 539
• Identity: 203/539 - (37%)
• Similarity: 289/539 - (53%)
• Gaps: 70/539 - (12%)

Human   109 PGGEAERVRVFHK--QAFEYISIALRIDEDEK-AG---QKEQAVEWYKKGIEELEKG-----IAV 162
            |.|.:...||..|  ..:|.:..|:.:||..| ||   :|.:..|.||:....|::.     :.:
 Worm     8 PAGSSTYDRVAQKFQDGYEKMRAAIEMDELTKHAGSIQEKLRTAELYKEARSLLKEANEFNIMDI 72

Human   163 IVTGQGEQCERARRLQAKMMTNLVMAKDRL-QLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQ 226
            ..|.:.|    .|..:..||.....|:||| .:..::.|.:..|:|.|  ...|...:......:
 Worm    73 PETRRSE----IRDKRQNMMKLEKSAQDRLIAICNEVDPNVKQSRSAT--VGPSRPASAARVTPR 131

Human   227 SESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKG 291
            ......|::|:......|.  .::.|...|.. ...||:                    |.|
 Worm   132 PTRATAPEKKNAAKAKEND--ENRHVCSRGDR-CGAHHQ--------------------PVT--- 170

Human   292 TPKTNRTNKPSTPTTAT-RKKKDLKNFR--------------------NVDSNLANLIMNEIVDN 335
              |.:.|..|..|..|: ||.:.:|..:                    .||..:...:::|::||
 Worm   171 --KKSDTVHPEPPVQASNRKMETVKRVKVDKASLPMHQNPVNRAALLNGVDKVIGERLLDEVLDN 233

Human   336 GTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESN 400
             |.|:.||:||...||.||:|.||||:|.|.||.|||.|.:|:|||||||||||:||||||.||.
 Worm   234 -TGVRMDDVAGCHSAKAALEEAVILPALNPNLFKGLRQPVKGILLFGPPGNGKTLLAKAVAGESK 297

Human   401 ATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEF 465
            ..||||||:|||||:||:.||.:|.||.:||..|||||||||:||:||||.|.:.:.|||:||||
 Worm   298 QMFFNISASSLTSKWVGDSEKTIRGLFQIARNAQPSIIFIDEIDSILCERSEKDAEVSRRMKTEF 362

Human   466 LIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQG--SP 528
            |::|||..|:.|||:||:||||||.|||:||||||.||:.::||:||.|..|:...|.|..  ..
 Worm   363 LVQFDGATSSADDRILVIGATNRPHELDDAVLRRFPKRIMLNLPDEEARKELITKTLKKHNMMDG 427

Human   529 LTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIK 593
            |...::..:|..|.|:|.|||.||.|:||:.||||:...::......::|.||.|||..:|:.|:
 Worm   428 LISSDIRYIASNTSGFSNSDLVALCKEAAMVPIREIDRSKLSMTDGEKIRKIRASDFDTALRTIR 492

Human   594 RSVSPQTLEAYIRWNKDFG 612
            .|.|.:.:.....:::.||
 Worm   493 PSTSQKIMSKLSDFSRSFG 511

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		42/202 (21%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194		26/96 (27%)
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196		24/95 (25%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200		24/97 (25%)
		MIT_spastin	116..195	CDD:239142	24/90 (27%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266		7/41 (17%)
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		172/408 (42%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		12/78 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		9/34 (26%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		2/2 (100%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		1/1 (100%)
		RecA-like_spastin	343..506	CDD:410932	110/162 (68%)
		AAA_lid_3	533..587	CDD:465537	22/53 (42%)
		Vps4_C	<580..612	CDD:462762	9/31 (29%)
spas-1	NP_001256115.1	cell_div_CdvC	175..510	CDD:468935	160/335 (48%)
		P-loop containing Nucleoside Triphosphate Hydrolases	240..401	CDD:476819	109/160 (68%)