Protein Alignment SLC12A3 and VHC1

DIOPT Version :10

• Sequence 1: NP_000330.3
• Gene: SLC12A3 / 6559
• HGNCID: 10912
• Length: 1030
• Species: Homo sapiens
• Sequence 2: NP_009794.3
• Gene: VHC1 / 852537
• SGDID: S000000439
• Length: 1120
• Species: Saccharomyces cerevisiae
• Alignment Length: 841
• Identity: 225/841 - (26%)
• Similarity: 367/841 - (43%)
• Gaps: 151/841 - (17%)

Human   125 SSEKNPEEP--VRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGL 187
            ||:.:|:.|  .:.|...||.:...||:..::::||..:|..|.||:.|..::|||.|:..:|.|
Yeast    50 SSKYDPDNPNKDKLGTYDGVFVPTALNVLSILMFLRFGFILGQLGIICTIGLLLLSYTINLLTTL 114

Human   188 SISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYG-- 250
            |||||||||.|:.||.|::||||||||.||||||:|.........|:.||..|   .||...|
Yeast   115 SISAISTNGTVRGGGAYYMISRSLGPEFGGSIGLVFFLGQVFNAGMNAVGIIE---PLLYNLGYS 176

Human   251 ------APIVD--PINDIRIIAVVSVTVLLAISLA--GMEWESKAQVLFFLVIMVSFANYLVGTL 305
                  |.:.:  |..........:|.:.|..|:|  |.:..|:|..:.|||:..|..:..:..|
Yeast   177 AQGEPPAALGELLPRGHWHEFTYATVILFLCFSVAFVGSQTVSRAGNILFLVLAASIFSIPLSAL 241

Human   306 IPPSEDKASKGFFSYRA---DIFVQNLVPD-WRGPDG-------TFFGMFSIFFPSATGILAGAN 359
            |   ....::|..||..   ..|..||:|. .:|..|       ||..:|.:|||:..||.|||.
Yeast   242 I---RSPFTEGGISYTGPSWQTFHDNLLPHLTKGAAGSLLKGKETFNDLFGVFFPATAGIFAGAG 303

Human   360 ISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYG 424
            :|.:|:.|:.:||||||..:.:|.|.|..:..::|
Yeast   304 MSSELRKPSKSIPKGTLWGLLFTFICYAVVVFSMG------------------------------ 338

Human   425 WNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLY 489
                 |:......|..:...||:|.|..   :|..|....:|.|.:..::.||.|.:.:.:|.:.
Yeast   339 -----CSIPRRSLYDEVQIIQTISSVQW---VIFMGEMATSLFSIIVGMLGAAYVLEAIAKDNII 395

Human   490 PLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITN 554
            |.:..|.      ..|:...:..: |.....:.:::|.||..|:..||.::.::|.:||...|::
Yeast   396 PGLEIFA------HSPLYSLIFTW-ILTQLCLFSDVNKIATFITMTFLMTFVVMNLACFLLGISS 453

Human   555 SPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAG 619
            :|.:||||:|:|::....||::|||.|.::...:|.:....::.|.|::.|..|..:||...|:.
Yeast   454 APNFRPSFKYFNRYTTAIGALLSVVAMLIVDGISASVLFLAMILLFLFIHYFSPPKSWGDVSQSL 518

Human   620 SYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLI-GPHK 683
            .|:....|.:.|.  :|:||.:|||.|:....|.....|:.|.....:. .|.|.|||.: ....
Yeast   519 IYHQVRKYLLRLR--QDNIKYWRPQILLFVDNPRTSWNLIRFCNHLKKG-GLYILGHVAVTADFP 580

Human   684 QRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQS 748
            :::.||:.......|..:...||||........|..|::.:...:|||.|||||.||||
Yeast   581 KQLNELKTQQKAWMKIRDMAAIKAFVQVGTGPSLIWGIRNVFIGSGLGGMKPNITVVGF------ 639

Human   749 AHPATVEDYIGILHDAFDF-NYGVCVMRMREGLNVSKMMQ------------AHIN-PVFDPAED 799
                            ||. :|...:.:.|...|:.|.::            ..|| |:  |.::
Yeast   640 ----------------FDLESYRKHIPQSRSQNNLQKQVEIKATVPRSTCSDVKINVPL--PTDE 686

Human   800 GKEASARGARPSVSGALDPKALVKEEQATTIFQSEQ---------GKKTIDIYWL---------- 845
            .|..:....:..|....|...:.........|::.:         .|||||:|.:
Yeast   687 CKNETKVNVQQWVQIVEDLSLMQSNIAIAHGFKNLEIPNKRDSCFPKKTIDLYPIQMCGKVEAKG 751

Human   846 ---------FDDGGLTLLIPYLLGRKRRWSK---CKIRVFVGGQINRMD--QERKAIISLL 892
                     ||...|.|.:..:|.....|.:   .::.:||..:.:|.:  |..|.::.:|
Yeast   752 DQPAAITTNFDTYTLILQLAAILVTVPEWKRTHSLRVILFVEQEYHRTNETQRMKKLLQVL 812

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SLC12A3	NP_000330.3	2a30	59..1029	CDD:273347	225/841 (27%)
		Scissor helix. /evidence=ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT	615..630		3/14 (21%)
VHC1	NP_009794.3	AA_permease_2	45..824	CDD:459263	225/841 (27%)