Protein Alignment SLC12A3 and nkcc-1

DIOPT Version :10

• Sequence 1: NP_000330.3
• Gene: SLC12A3 / 6559
• HGNCID: 10912
• Length: 1030
• Species: Homo sapiens
• Sequence 2: NP_001255790.1
• Gene: nkcc-1 / 3565648
• WormBaseID: WBGene00012543
• Length: 1235
• Species: Caenorhabditis elegans
• Alignment Length: 1180
• Identity: 450/1180 - (38%)
• Similarity: 652/1180 - (55%)
• Gaps: 214/1180 - (18%)

Human     5 PTTETPGDATLCSG-RFTISTLLSSDEPSPPAAYDSSHPSHLTH--SSTFCMRTF-GYNTIDVVP 65
            |..::|  |:...| .|::     .|:.|...:.|.....::||  .:||.|::: ...||:..|
 Worm   113 PAEKSP--ASRAKGVHFSV-----GDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPP 170

Human    66 TYEHYANSTQPGEPRKVRPTLADL-----H-----SFLKQEGRHLHALAFDSRPSHEMTDGLVEG 120
            ..:.|.||........ ||::|.|     |     .|.:...:|.|   .:..|.||
 Worm   171 IIDFYRNSIDTDGVFN-RPSMAQLIHGKEHKEQDLGFEELNAQHHH---LEDSPVHE-------- 223

Human   121 EAGTSSEKNPEE--------PVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILL 177
               |.:|...|:        .|:|||::||.:||:|||:||:||||:.|::.|||:.|...|:||
 Worm   224 ---TKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLL 285

Human   178 SVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETV 242
            :..||:||.||..||.|||.||.||.|||||||||||.|||||:||:.|||||.||:.|||||:.
 Worm   286 ASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESF 350

Human   243 RDLLQEY--GAPIVDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTL 305
            ||:|.:|  |......:.|:|:|..|:..:|:.|...|.|:|||.|:...::::||.|||:.|:.
 Worm   351 RDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSF 415

Human   306 IPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIA 370
            .||:|...::|...|..|....|.:|.:. .:.|||.:||::||:||||:|||||||||.:|..|
 Worm   416 FPPNEIAMNRGATGYSLDTLQSNFMPHFT-ENNTFFSVFSVYFPAATGIMAGANISGDLANPQQA 479

Human   371 IPKGTLMAIFWTTISYLAISATIGSCVVRDASG----VLNDT--VTPGWGACEGLACSYGWNFTE 429
            ||.|||:||..|||.||......||.||..::|    |.|::  |.|                 :
 Worm   480 IPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPP-----------------D 527

Human   430 CTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGF 494
            ||.  .|.:||::|||.:.|.|.:.||||||||.||||||||.||||.||||.:|:|:|:|.|.:
 Worm   528 CTP--DCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDY 590

Human   495 FGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWR 559
            |.|.||||:||.|.|:|.:.:|:..:.|.:||.||||||||||.||||||::||..|..:|||:|
 Worm   591 FAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFR 655

Human   560 PSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLA 624
            |.|.|||.|.:|.||::.|.:||::.|::||:.......:.:|::::||:||||||.||.||..|
 Worm   656 PGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNA 720

Human   625 LSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVL-IGPHKQRMPE 688
            ||..:.|:..|:|:||||||.|||:|.|..|..||||....|:..||::||.|: ..|..:....
 Worm   721 LSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMV 785

Human   689 LQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPA- 752
            ::.:......||.||.:||||..|...:.|:|.|.|:|..|:.:|||||:::|||.||....|.
 Worm   786 MKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTE 850

Human   753 ----TVEDYIGILHDAFDFNYGVCVMRMRE-GLNVSKMM---------QAHINPVFD-------- 795
                .:.:|.|.:.|.||:|..:||:|..: ||:.|:.|         :.::.|:.:
 Worm   851 TNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKT 915

Human   796 ---------------------PAEDGKEA--SARGARPSV------------------------- 812
                                 ..:||..|  |.||:..||
 Worm   916 NENSPETVHLIEKDETARTDKSGDDGSSARLSYRGSCRSVEMFQRINAFYINASISINETYVSDE 980

Human   813 -----------------SGA----------------------------------LDPKALVKEEQ 826
                             |||                                  ::.|||:...|
 Worm   981 NQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQ 1045

Human   827 ATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSK-CKIRVFVGGQINR-MDQERKAII 889
            .   |..:..|.|||::||:||||||||||:||...:.:.: .::|:|.....:| |:||::.:.
 Worm  1046 R---FNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMA 1107

Human   890 SLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDE- 953
            :||||||:.:.:|:::.||.:.||.|....::.:|.||...||            .||..::.:
 Worm  1108 ALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDG------------SCPIGMTTKS 1160

Human   954 EITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIR 1018
            |::..|.|:.||:|..|::.::|.:|.|||:|||:.|||...|||||:|||.::::| |||:|:|
 Worm  1161 ELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNL-PPVLLVR 1224

Human  1019 GNQENVLTFY 1028
            |||::|||||
 Worm  1225 GNQQSVLTFY 1234

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SLC12A3	NP_000330.3	2a30	59..1029	CDD:273347	437/1122 (39%)
		Scissor helix. /evidence=ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT	615..630		8/14 (57%)
nkcc-1	NP_001255790.1	AA_permease_2	165..1235	CDD:459263	437/1121 (39%)