Protein Alignment SLC12A2 and NKCC

DIOPT Version :10

• Sequence 1: NP_001037.1
• Gene: SLC12A2 / 6558
• HGNCID: 10911
• Length: 1212
• Species: Homo sapiens
• Sequence 2: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Alignment Length: 1151
• Identity: 457/1151 - (39%)
• Similarity: 664/1151 - (57%)
• Gaps: 162/1151 - (14%)

Human   138 RFRVNFVDPAASS-------SAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGH 195
            ||:::.|:..|::       |||...:|||.   ..|..|.:|          .||....|.:|.
  Fly     4 RFQISKVNGTANAGYDGEARSAESDQTDAAH---PLPPTSNEN----------HLHPKASGETGE 55

Human   196 HQHYYYDTHTNTYYLRTFGH---------------NTMDAVPRIDHYRHTAAQLGEKLLRPSLAE 245
            ::      .::....|.||:               .|.:::||:|:||.:.    ..|.|||:.|
  Fly    56 NR------RSSRLSFRGFGNFLRKSDAERKFSLAQLTKESLPRLDNYRISM----RNLKRPSIGE 110

Human   246 LHDEL--------EKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGV 302
            |..|.        |.||...|                      |.:|.|||.|||:.|:||||||
  Fly   111 LQGEAVDQSITIPEPEPEATG----------------------GHIKLGWIVGVLIPCLLNIWGV 153

Human   303 MLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGA 367
            |||:||||:|.::||..|:::|.::.||..||.||.|||:|||.|:|||.|::||||||||||.:
  Fly   154 MLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGAS 218

Human   368 IGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDE-INDIRIIGAITVVILLGISVAGMEWE 431
            :|::|||||||:.:|..:||.|::..|||.:.:.::|. ||||||:|:|||::|:.|...|||||
  Fly   219 VGVVFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWE 283

Human   432 AKAQIVLLVILLLAIGDFVIGTFI-PL--ESKKPKGFFGYKSEIFNENFGPDFREEE----TFFS 489
            .|||..|:|.::|||.:|:||..| |.  |.:..:||.|:......||||.|:|..|    .|||
  Fly   284 TKAQNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFS 348

Human   490 VFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATG---- 550
            |||||||:.|||.|||||.|||.|..:||||||..::||:...|....:..|...||||:|
  Fly   349 VFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPAD 413

Human   551 NVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSA 615
            .||.|||:....|.:             :..|::||.|::::|..:|.:.|||.||.|:||||:|
  Fly   414 LVNGTIVSSELPCMA-------------TGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTA 465

Human   616 LASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISN 680
            |.:|:|.|::.|||..|.|||....|:|.|||:.||.|||:|||.|..||:||.|||:|||:||.
  Fly   466 LTNLLSVPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLIST 530

Human   681 FFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGL 745
            |:|||||||||..|||:..|..||||.|||||.|:||.|..:|..:||:||:.||::|:.|:..|
  Fly   531 FYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFAL 595

Human   746 YIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHD 810
            |:.|.|:||:.||||:|||..|..||....||..|.|||||:.||.||::|.|.:||.|:...:.
  Fly   596 YLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYL 660

Human   811 FTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAG 875
            .|||..||...::.  |.|...|.........|::|...|:||||..:....|.:|...|.::.|
  Fly   661 LTKNNSLMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTG 723

Human   876 LGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLS- 939
            .|:|.||.:::|:|.||.:....:|:.|.::.::||..:.||.::||..|||.|.|..:..|.:
  Fly   724 FGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPAN 788

Human   940 -------------SQEKSPGTKDV-----------VVSVEYSKKSDLDTSKP-----LSEKPITH 975
                         :.|..|.....           :.|:: |..|.....:|     :.....::
  Fly   789 GMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMD-SPNSSFTMPQPAPMPNMQRNSRSY 852

Human   976 KVEEEDGKTATQPLLKKESK-GPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLI 1039
            ||...|     :|.:...:| |..:|     |.||:|.|.|.:||.|.||||:||:||||||:|:
  Fly   853 KVTSSD-----EPAVTYHTKGGSDIP-----QNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILL 907

Human  1040 PYLLTTKKKWKDCKIRVF--IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENII 1102
            ||:::.:..|::.|:|||  ..||..  :.:.::||:||:||||.:|::::|..::.:|:.:.::
  Fly   908 PYIISMRSHWQNSKLRVFAMCHGKDE--EQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVL 970

Human  1103 AFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIV 1167
            ..:.:|||:|....::             :.|||:||:....||.||:|::||:.:|||.|:::|
  Fly   971 KHKRLIEPFRRGARNE-------------FGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVV 1022

Human  1168 MSLPVARKGAVSSALYMAWLEALSKDLP-PILLVRGNHQSVLTFYS 1212
            ||||:.||.|:|:.|||:|||.|:.|:. |:.|.|||...|||.||
  Fly  1023 MSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SLC12A2	NP_001037.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	36..81
		RFXV motif 1. /evidence=ECO:0000269|PubMed:16669787	80..83
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	112..138		457/1151 (40%)
		RFXV motif 2. /evidence=ECO:0000269|PubMed:16669787	138..141		2/2 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	150..193		12/49 (24%)
		2a30	216..1212	CDD:273347	435/1049 (41%)
		Scissor helix. /evidence=ECO:0000269|PubMed:35585053, ECO:0000269|PubMed:36306358, ECO:0007744|PDB:7MXO, ECO:0007744|PDB:7N3N, ECO:0007744|PDB:7S1X, ECO:0007744|PDB:7SFL, ECO:0007744|PDB:7SMP	761..778		7/16 (44%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	962..989		4/31 (13%)
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	435/1049 (41%)