Protein Alignment SLC12A2 and nkcc-1

DIOPT Version :10

• Sequence 1: NP_001037.1
• Gene: SLC12A2 / 6558
• HGNCID: 10911
• Length: 1212
• Species: Homo sapiens
• Sequence 2: NP_001255790.1
• Gene: nkcc-1 / 3565648
• WormBaseID: WBGene00012543
• Length: 1235
• Species: Caenorhabditis elegans
• Alignment Length: 1273
• Identity: 520/1273 - (40%)
• Similarity: 723/1273 - (56%)
• Gaps: 198/1273 - (15%)

Human    58 DEGPAAAGDGLGRPL-----GPTPSQSRFQVDLVSEN---AGRAAAAAAAAAAAAAAAGAGAGAK 114
            ||....:.|.| |.|     .|.|:|:...|.||:..   .||....:........|..|.....
 Worm    43 DEDEKTSDDNL-RKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGI 106

Human   115 QTPADGEASGESEPAKGSEEAKG-RFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTV 178
            ..|.|..|  |..||   ..||| .|.|.  |..:.|.:||        |.:..|::.:|
 Worm   107 DVPIDPPA--EKSPA---SRAKGVHFSVG--DKDSGSMSED--------GDEKRNITHEN----- 151

Human   179 LSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGH-NTMDAVPRIDHYRHTAAQLGEKLLRPS 242
                                      ..|:.::::.: .|::..|.||.||::....| ...|||
 Worm   152 --------------------------QTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDG-VFNRPS 189

Human   243 LAEL-HDELEKEP---FEDGFANGE----ESTPTRDAVVTYTAE------SKGVVKFGWIKGVLV 293
            :|:| |.:..||.   ||:  .|.:    |.:|..:....|..|      |:..||||||:||.|
 Worm   190 MAQLIHGKEHKEQDLGFEE--LNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFV 252

Human   294 RCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISR 358
            ||:|||:||||::|:||:.||||:||...::::|:||||||.|||.||.|||.|:|||||:||||
 Worm   253 RCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISR 317

Human   359 SLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDE--INDIRIIGAITVVILL 421
            ||||||||:||:||:.||||..|||:|||||:..::|.:::|..|.:  :.|:|:||.:|.|||:
 Worm   318 SLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILM 382

Human   422 GISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFI-PLESKKPKGFFGYKSEIFNENFGPDFREEE 485
            ||...|.|:|:|.|:.||||||::|.::|.|:|. |.|....:|..||..:....||.|.|.|..
 Worm   383 GIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENN 447

Human   486 TFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATG 550
            ||||||:::|||||||:||||||||||:||.|||.|||||||:||:||:......||.||
 Worm   448 TFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVV----- 507

Human   551 NVNDTIVTELTNCTSAACKLNFDFSSCESSP-CSYGLMNNFQVMSMVSGFTPLISAGIFSATLSS 614
                    ..:|.|..|...|..|...:.:| |.:||::.:||:.|.|.:.|||:||||:|||||
 Worm   508 --------AFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSS 564

Human   615 ALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIIS 679
            ||||||||||:|||:|||.::|....|||.||||.||.|.|:|.|.:|:|.:.|.:|||||||||
 Worm   565 ALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIIS 629

Human   680 NFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLG 744
            ||||.||||||::.|..|.|.|||:||.|.||||||||:||:||..|||:|:|::||:|:.....
 Worm   630 NFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGA 694

Human   745 LYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVH 809
            :::|:.::||||||||||||.:|.|||...|:||..|:||||:|||.||::|.|.||..|:...:
 Worm   695 IFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFAN 759

Human   810 DFTKNVGLMICGHV--HMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQ 872
            :.||...|::||.|  :....|..|....:|: ....||.|..:||||..|...:.|:|||.|:|
 Worm   760 NITKGSSLLVCGQVVPYEPSDRVYMVMKRLDE-NINVWLRKRHLKAFYRAVANSNFRKGAQSLIQ 823

Human   873 AAGLGRMKPNTLVLGFKKDW-----LQADMRDVDMYINLFHDAFDIQYGVVVIRLKE-GLDISHL 931
            ..|:.:||||.:::|||.:|     .:.::.:::.|.....|.||....:.|:|..: |||.|..
 Worm   824 LTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEA 888

Human   932 ------------------QGQEELLSSQEKSPGTKDVVVSVEYS---KKSDLDTSKPLSEKPITH 975
                              :.::|...:.|.||.|..::...|.:   |..|..:|..||.:....
 Worm   889 MRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSARLSYRGSCR 953

Human   976 KVE-----------------------------------EEDGKTATQPLLKKESKGPIVPLNVAD 1005
            .||                                   :.||:.:...  .:..|...|.|.|.|
 Worm   954 SVEMFQRINAFYINASISINETYVSDENQEDADGDDDNDNDGEESGAD--DEHQKEDDVELGVID 1016

Human  1006 QK---------------------LLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKW 1049
            ::                     ||.:..:|.:|..|.|||||||:||||||||||:||...|.:
 Worm  1017 EQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSY 1081

Human  1050 -KDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYR 1112
             :..::|:| |......::.::|.||.||||||||:||:.|:.||..||::|.:..::.:|:|:
 Worm  1082 LEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPF- 1145

Human  1113 LHEDDKEQDIADKMKEDEPWRI---TDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVAR 1174
                         :.:|....|   |.:||...:.|||||:|..|||.|||..|::|||:|||.|
 Worm  1146 -------------IAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPR 1197

Human  1175 KGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS 1212
            ||.|||:|||:|||.::::|||:||||||.||||||||
 Worm  1198 KGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS 1235

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SLC12A2	NP_001037.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	36..81		9/27 (33%)
		RFXV motif 1. /evidence=ECO:0000269|PubMed:16669787	80..83		0/2 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	112..138		9/26 (35%)
		RFXV motif 2. /evidence=ECO:0000269|PubMed:16669787	138..141		1/2 (50%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	150..193		6/42 (14%)
		2a30	216..1212	CDD:273347	483/1103 (44%)
		Scissor helix. /evidence=ECO:0000269|PubMed:35585053, ECO:0000269|PubMed:36306358, ECO:0007744|PDB:7MXO, ECO:0007744|PDB:7N3N, ECO:0007744|PDB:7S1X, ECO:0007744|PDB:7SFL, ECO:0007744|PDB:7SMP	761..778		9/16 (56%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	962..989		7/61 (11%)
nkcc-1	NP_001255790.1	AA_permease_2	165..1235	CDD:459263	483/1102 (44%)