Protein Alignment eIF2D and TMA64

DIOPT Version :9

• Sequence 1: NP_477481.1
• Gene: eIF2D / 64874
• FlyBaseID: FBgn0041588
• Length: 563
• Species: Drosophila melanogaster
• Sequence 2: NP_010402.3
• Gene: TMA64 / 851695
• SGDID: S000002524
• Length: 565
• Species: Saccharomyces cerevisiae
• Alignment Length: 639
• Identity: 147/639 - (23%)
• Similarity: 252/639 - (39%)
• Gaps: 150/639 - (23%)

  Fly     1 MFLKPYRPKSSAALKGSDSKKFRQ-------RVEAAFPHVSVDQLVPAKAAVTQVKILTHGGVQS 58
            ||.|....|:.:.||.|:.||..|       ..|.:|             ..:.:|.....|.:|
Yeast     1 MFKKEPHIKALSNLKNSERKKLLQTFQKQTNNEEYSF-------------RTSTIKQTNFNGQKS 52

  Fly    59 M--VYC-VDKLPLFF-ELDGGQLVPTLYTLWIVPDILPYFTTHEGVLPK-LTNGADLMLPGVVPL 118
            :  ||. .:..|:.| |....||.||:|:.|..|.:||...||..|:.: |.|||:||:.|.:|
Yeast    53 VGTVYTDENNTPILFKEKHKEQLFPTVYSCWEYPALLPIVLTHGFVIEEHLFNGANLMISGSIP- 116

  Fly   119 GVGLSMYGHFKKGQLMAV---NLTNNKSAVGVGQLARSSDELYMCGGHGVAIKMLHLFGDKLWSH 180
                ......|.|.|..:   .......|:|:.:|...|.: .:.|..|||:|::|.|.|.|
Yeast   117 ----PFDPRCKIGTLCGIASKQAPETVLAIGIVELDLPSFD-KVIGETGVAVKIIHHFNDGL--- 173

  Fly   181 EPSLVQQIPLVKTKALSGEDFPALGSETERRKTASPATPAPVTFATVAQTEELETETAALTLESQ 245
                                     |:..:.|...|         .|..|:..:...::..:||.
Yeast   174 -------------------------SKVFKMKLEPP---------FVLSTQSKDNNISSKQIESS 204

  Fly   246 E---APESDEEAVEEASPEL-----ILKNAFLSALK-----------NHGKKLPLPLLTSNFYRL 291
            |   |.|.::|.|:|||.::     :|.:..:|.:.           ...|.|.||:..|||...
Yeast   205 EQIKAVEKEQEDVKEASVDVEEIAEVLDHFTVSDVDYFITRALYYTLTQDKGLELPISASNFISN 269

  Fly   292 YVVTEAT----EQIDLKKTRYKKLSNFLAEMVDQGFIVVREESKGVD-KITSVDLEHPEVVNFI- 350
            :::....    .::::|||.:||.:.||.....:||:.::  .||.| .|...:.:..|:.||:
Yeast   270 HIMRNLPPIDHNEVNVKKTSWKKSAKFLKHFEKEGFLKLK--GKGDDLTIVGKNTDKDELKNFVP 332

  Fly   351 ----TDVKASEANGAASETPLFHSELKE-----MYIVT-----DVTAAFFTKLNYKRGEGIPAGQ 401
                ....|:|:..:.:.        ||     ||.:|     ::......::|........:..
Yeast   333 YKLGCSKSATESRESTTS--------KEKTSGMMYSLTLYKPFNLAKDLLKEVNLASHTYYTSQD 389

  Fly   402 IKKIVREYVSKHGLLHPLTK-LVRPDDTLIELYGNREVSLSE---------MCSLITSKMEHSYQ 456
            |:..|.:|:|...|.....| .|..||.|.::...::..|:.         :..|:|:.....||
Yeast   390 IRSAVSQYISVKNLADTKDKGKVIMDDLLFDMVNKKKKVLNASRIIARGEILHPLLTNNFTEFYQ 454

  Fly   457 MCSGKDT--------SGKPLIQMSLATRSGNKKVTLVSNIEAYGI----ILAEFIKLCKQGAAAS 509
            :....||        ...|.|::....:.|.|.:|.|||.|.:.:    :.|:..|:|    :.|
Yeast   455 IFKSDDTLLFKAPMKGSLPHIKIITEMKIGRKVITRVSNFEVFQVDPESLAADLRKIC----SGS 515

  Fly   510 TSVVKLPHQKHEQLQIQGNQVRFVHTLLTETYKVPPKCILGLELAKDGKKNKKK 563
            |::.:....|..::|:||...:.:...|.: ..:|.|.|   :.....||.|:|
Yeast   516 TTISESQTFKCAEVQVQGPHGQSIIDHLNK-LGIPSKWI---DFENKLKKKKRK 565

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eIF2D	NP_477481.1	eIF2D_N	6..79	CDD:211423	19/83 (23%)
		Tma20	9..178	CDD:224927	52/183 (28%)
		PUA	61..175	CDD:294405	38/119 (32%)
		eIF2D_C	469..551	CDD:211321	21/85 (25%)
TMA64	NP_010402.3	Pre-PUA	2..86	CDD:407697	25/96 (26%)
		PUA_eIF2d-like	88..170	CDD:409298	26/87 (30%)
		eIF2D_C	474..556	CDD:211321	21/89 (24%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157344842
Domainoid	1	1.000	49	1.000	Domainoid score	I2947
eggNOG	1	0.900	-	-		E1_COG2016
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	88	1.000	Inparanoid score	I1568
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG55541
OrthoFinder	1	1.000	-	-		FOG0005549
OrthoInspector	1	1.000	-	-		oto99111
orthoMCL	1	0.900	-	-		OOG6_102936
Panther	1	1.100	-	-	LDO	PTHR12217
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3687
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.790