Protein Alignment ITSN1 and itsn1

DIOPT Version :10

• Sequence 1: NP_003015.2
• Gene: ITSN1 / 6453
• HGNCID: 6183
• Length: 1721
• Species: Homo sapiens
• Sequence 2: XP_005171028.1
• Gene: itsn1 / 368504
• ZFINID: ZDB-GENE-030616-226
• Length: 1751
• Species: Danio rerio
• Alignment Length: 1787
• Identity: 1364/1787 - (76%)
• Similarity: 1498/1787 - (83%)
• Gaps: 102/1787 - (5%)

Human     1 MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPI-SGFITGDQARNFFFQSGLPQPVLAQIWAL 64
            ||||||||||..|.|.|:|:||||||||||||.|. :||||||||||||.|||||.|:|||||||
Zfish     1 MAQFPTPFGGGSDTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWAL 65

Human    65 ADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAV 129
            |||||||:||..|||||||||||||||:.||.:|||.|||||::|.....||:..:.:||.|.||
Zfish    66 ADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGIPSMGAMPGLPAV 130

Human   130 APVPM----GSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGP 190
            .|:|:    ..:|.:|:||.|.:|| |..||.||||||.:||||..|:||.|.|.|:.||:||
Zfish   131 PPMPLPPLPPGVPGMGVSPPLGASV-TPPVPSLANGAPAMIQPLSGFSHPGAVLNKTPSFNRS-- 192

Human   191 GSQLNTKLQKAQSFDVAS---VPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQS 252
                :.||||.||.:..|   .||.|:|||.||||||||||||||||.|||:|||||||||||||
Zfish   193 ----SIKLQKGQSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQS 253

Human   253 SLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGI 317
            ||||||||:||||||||||||||||||||||||||:||||.||||:|||:.|||:|||:|||||:
Zfish   254 SLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGV 318

Human   318 SVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQER 382
            ||.|..|.|.|..|||  |:|:::.||||||||||||||||||||||||||||||||||||||||
Zfish   319 SVTSMHSTDLRSQEEP--EEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQER 381

Human   383 LAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQL 447
            ||.||:.|||||||||.||||:||.||:||||:||||||||||||||||||||||||||||||||
Zfish   382 LAALEKEEQERKERERLEQERRRQQELDKQLERQRELERQREEERRKEIERREAAKRELERQRQL 446

Human   448 EWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIES 512
            ||||.||||||.|||:|||:||:|||:|||||||||||||||.||||||||||.||:.||.|||:
Zfish   447 EWERTRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRSRLSIQRHEIET 511

Human   513 TNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKEL 577
            |||:|||||||||.||||||||||.|.||||:||.||||||||||||||||||.:|:||:|.||.
Zfish   512 TNKTRELRIAEITSLQQQLQESQQWLSRLIPDKQCLNDQLKQVQQNSLHRDSLNSLQRAIEMKES 576

Human   578 ARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIEL 642
            .:|.|::|||.|||||||||.|||.||.|||||||||||||.||||.:||: :.|...:||.|:.
Zfish   577 TKQQLKEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEAD-ITQPSHDRKSIDS 640

Human   643 EKQK----EEAQRRAQERD-----------KQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKD 692
            :..:    ::....|...|           :.|:..|.:|:.|.|
Zfish   641 QDSRLSGTDDGVSPAWRDDGLGKAPTPPVSQAWMSRVSEEENHSR-------------------- 685

Human   693 GEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHD 757
                  .|.||.|.:||.||.:..|...|:|||.|:|.|::...||.||||||||:||||:||||
Zfish   686 ------AEIQDNLSKLFTQHPQAGKIQAQSPWSMADKIPVSGFNQEKVKVVYYRAMYPFEARSHD 744

Human   758 EITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSA 822
            ||||.||||||||||||||||||||||||||:|||||||||||||||||:|||..::....|:||
Zfish   745 EITIHPGDIVMVKGEWVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLSLRASAASSSA 809

Human   823 P---------------APKLALRETPAPLAVTSSEP---------STTPNNWADFSSTWPT-STN 862
            |               .|.|.:...||.:|..||.|         |:..:||||||:|||: |..
Zfish   810 PKLGSHMSSASSSTTATPILPVSTEPASIAPPSSAPPPTGPASSSSSASSNWADFSTTWPSNSAV 874

Human   863 EKPETDNWDAW---AAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPW 924
            ||.::|.||||   ..||||:|||.||:||||||||||.:|||||||||||||||||||||||||
Zfish   875 EKQDSDGWDAWPTQPTQPSLSVPSGGQIRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPW 939

Human   925 RAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPAS 989
            |||||||||||||||||||||||||||||||||:||||||||||||||:|||.|::||||:||.|
Zfish   940 RAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIESGSSDSPPS 1004

Human   990 LKRVASPAAKPVVSGE-------------EFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTG 1041
            :||.:....||...||             |::|||||||:|||||||||||||.||||:||||||
Zfish  1005 VKRPSPSLNKPTELGEGQNSNSNSMYPSKEYVAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTG 1069

Human  1042 TVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKK 1106
            ||..|.||||||||:.|:|||.|:||||||||||||||||||.|||||.||||||||||||||||
Zfish  1070 TVSGKIGVFPSNYVKPKESEGLGSAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKK 1134

Human  1107 NPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTE--PPKSTALAAVCQVIGMYDYTAQ 1169
            ||||||||||||||||||||||||||||||||.|||.|||:  |||.....||||||||||||||
Zfish  1135 NPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQ 1199

Human  1170 NDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPT 1234
            |||||.|.||||||||::|||||||||:||.||||||||||||||.|||||||:||||||||||.
Zfish  1200 NDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPV 1264

Human  1235 ERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKAL 1299
            |||||||||||||||||||||||||||.|||||:||:|||||||||||||||||.||||||||||
Zfish  1265 ERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKAL 1329

Human  1300 RVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDP 1364
            |||||||||:||||||||||:||||||||||||||.|||||.||||||||.|:||:|||||||||
Zfish  1330 RVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDP 1394

Human  1365 RCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKEN 1429
            ||||||||||:|||||||||||||||||||||||:|||||||:.|||||||||||||||||||||
Zfish  1395 RCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVREKEN 1459

Human  1430 SDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITK 1494
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||.|
Zfish  1460 SDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIK 1524

Human  1495 PLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINER 1559
            ||||||||||||.||:|||:||||||||||||||||||||||||||||||||||||:||||||||
Zfish  1525 PLGSSGTDKVFSAKSHLQYRMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTIRAESINER 1589

Human  1560 TAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVT 1624
            |||||||||||||:|||||:||||||||||||||||||||||:|||||||||||:||||||||||
Zfish  1590 TAWVQKIKAASELFIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRSNGKSNPYCEVT 1654

Human  1625 MGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQ 1689
            ||||||:|||:||||||||||||||||:||||:|||:||||||||||||||||||||:|||||||
Zfish  1655 MGSQCHVTKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQ 1719

Human  1690 GSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP 1721
            |||||:||.|||||||||||||||||||||||
Zfish  1720 GSKGPITKRLLLHEVPTGEIVVRLDLQLFDEP 1751

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ITSN1	NP_003015.2	EH	14..108	CDD:197477	75/94 (80%)
		EH	214..309	CDD:197477	83/94 (88%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	322..348		12/25 (48%)
		KLERQ	326..702		260/390 (67%)
		Smc	<363..>684	CDD:440809	232/335 (69%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	650..701		7/61 (11%)
		SH3_Intersectin1_1	744..803	CDD:212920	54/58 (93%)
		INTAP	803..917	CDD:435467	72/141 (51%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	836..868		17/41 (41%)
		SH3_Intersectin1_2	917..968	CDD:212922	49/50 (98%)
		SH3_Intersectin1_3	1006..1057	CDD:212924	42/50 (84%)
		SH3_Intersectin1_4	1074..1138	CDD:212926	61/63 (97%)
		Required for interaction with FCHSD2. /evidence=ECO:0000269|PubMed:29887380	1074..1138		61/63 (97%)
		Bipartite nuclear localization signal, in isoform 2. /evidence=ECO:0000269|PubMed:29599122	1104..1127		22/22 (100%)
		SH3_Intersectin1_5	1158..1211	CDD:212928	46/52 (88%)
		RhoGEF	1238..1421	CDD:238091	167/182 (92%)
		PH_13	1440..1582	CDD:465218	134/141 (95%)
		C2_Intersectin	1582..1716	CDD:176021	123/133 (92%)
itsn1	XP_005171028.1	EH	15..112	CDD:197477	76/96 (79%)
		EH	215..293	CDD:197477	71/77 (92%)
		SMC_prok_B	438..>646	CDD:274008	157/208 (75%)
		SH3_Intersectin1_1	731..790	CDD:212920	54/58 (93%)
		INTAP	790..932	CDD:435467	72/141 (51%)
		SH3_Intersectin1_2	932..983	CDD:212922	49/50 (98%)
		SH3	1034..1085	CDD:473055	42/50 (84%)
		SH3_Intersectin1_4	1102..1166	CDD:212926	61/63 (97%)
		SH3_Intersectin1_5	1188..1241	CDD:212928	46/52 (88%)
		RhoGEF	1268..1451	CDD:238091	167/182 (92%)
		PH_13	1470..1612	CDD:465218	134/141 (95%)
		C2_Intersectin	1612..1746	CDD:176021	123/133 (92%)