DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ROBO3 and Robo3

DIOPT Version :9

Sequence 1:	NP_071765.2	Gene:	ROBO3 / 64221	HGNCID:	13433	Length:	1386	Species:	Homo sapiens
Sequence 2:	XP_006510170.1	Gene:	Robo3 / 19649	MGIID:	1343102	Length:	1403	Species:	Mus musculus

Alignment Length:	1403	Identity:	1193/1403 - (85%)
Similarity:	1265/1403 - (90%)	Gaps:	17/1403 - (1%)

- Green bases have known domain annotations that are detailed below.

Human     1 MLRYLLKTLLQMNLFADSLAGDISNSSELLLGFNSSLAALNHTLLPPGDPSLNGSRVGPEDAMPR 65
            ||||||||||||||||||||.|||||||||.||||||||||.:||||||||||||||||||||||
Mouse     1 MLRYLLKTLLQMNLFADSLARDISNSSELLFGFNSSLAALNPSLLPPGDPSLNGSRVGPEDAMPR 65

Human    66 IVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGALFFPRIVH 130
            ||||||||:||||||||||||||||||||||||||||||||.|||||||||||||||||||||||
Mouse    66 IVEQPPDLVVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVH 130

Human   131 GRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGHPE 195
            |||:||||||||||||||||||||||||||||||||||||||||||||||||||:|||||:||||
Mouse   131 GRRSRPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHPE 195

Human   196 PSVSWRKDGARLKEEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMVLERPSF 260
            |.|:|:|...:||||||||||||||||||||.|||||||:||||||||||||.|||::|||||||
Mouse   196 PLVTWKKGKIKLKEEEGRITIRGGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLERPSF 260

Human   261 LRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGHVSAEDEGTYT 325
            ||||:||||||||||.|||||:|||.|.|.|||:|||||.|||||||||||||..||:|||||||
Mouse   261 LRRPINQVVLADAPVNFLCEVQGDPQPNLHWRKDDGELPAGRYEIRSDHSLWIDQVSSEDEGTYT 325

Human   326 CVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLF 390
            |||||||||||||||||||||||.||:||:|..|||.:|:|||||||||||||||||||||||||
Mouse   326 CVAENSVGRAEASGSLSVHVPPQFVTKPQNQTVAPGANVSFQCETKGNPPPAIFWQKEGSQVLLF 390

Human   391 PSQSLQPTGRFSVSPRGQLNITAVQRGDAGYYVCQAVSVAGSILAKALLEIKGASLDGLPPVILQ 455
            |||||||.||..||||||||||.|:.||.||||||||||||||||||||||||||:|||||:|||
Mouse   391 PSQSLQPMGRLLVSPRGQLNITEVKIGDGGYYVCQAVSVAGSILAKALLEIKGASIDGLPPIILQ 455

Human   456 GPANQTLVLGSSVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQFKTMANGTLYIANVQEMDMGFYS 520
            |||||||||||||||||||.|||||:::||||.:||||||.||..|.||||:||::|||||||||
Mouse   456 GPANQTLVLGSSVWLPCRVIGNPQPNIQWKKDERWLQGDDSQFNLMDNGTLHIASIQEMDMGFYS 520

Human   521 CVAKSSTGEATWSGWLKMREDWGVSPDPPTEPSSPPGAPSQPVVTEITKNSITLTWKPNPQTGAA 585
            ||||||.|||||:.||:.:||||.||.|.|.||:|||.||||:|||:|.||||||||||||:||.
Mouse   521 CVAKSSIGEATWNSWLRKQEDWGASPGPATGPSNPPGPPSQPIVTEVTANSITLTWKPNPQSGAT 585

Human   586 VTSYVIEAFSPAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVRTQ 650
            .|||||||||.|||||||||||||||||:|:|||||||||||||||||||||||||||||||:||
Mouse   586 ATSYVIEAFSQAAGNTWRTVADGVQLETYTISGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVQTQ 650

Human   651 -DSSPSRPVEDPWRGQQGLAEVAVRLQEPIVLGPRTLQVSWTVDGPVQLVQGFRVSWRVAGPEGG 714
             |||.|||.||||:||:||||||||:|||.||||||||||||||||||||||||||||:||.:.|
Mouse   651 ADSSLSRPAEDPWKGQRGLAEVAVRMQEPTVLGPRTLQVSWTVDGPVQLVQGFRVSWRIAGLDQG 715

Human   715 SWTMLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGAESLSVTRSIPEEAPSGPPQGVAVAL 779
            ||||||||||.:|||||||||||.|||||||.||||||||||..|||||||||||||||||||||
Mouse   716 SWTMLDLQSPHKQSTVLRGLPPGAQIQIKVQVQGQEGLGAESPFVTRSIPEEAPSGPPQGVAVAL 780

Human   780 GGDGNSSITVSWEPPLPSQQNGVITEYQIWCLGNESRFHLNRSAAGWARSAMLRGLVPGLLYRTL 844
            |||.|||:|||||||||||:||||||||||||||||||||||||||||||....||:||.:||.|
Mouse   781 GGDRNSSVTVSWEPPLPSQRNGVITEYQIWCLGNESRFHLNRSAAGWARSVTFSGLLPGQIYRAL 845

Human   845 VAAATSAGVGVPSAPVLVQLPSPPDLEPGLEVGAGLAVRLARVLREPAFLAGSGAACGALLLGLC 909
            |||||||||||.|||||||||.||..|||.||..|||.|||:|||:|||||||.|||||||||.|
Mouse   846 VAAATSAGVGVASAPVLVQLPFPPAAEPGPEVSEGLAERLAKVLRKPAFLAGSSAACGALLLGFC 910

Human   910 AALYWRRKQRKELSHYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYSWLADSWPHPSRSPS 974
            ||||.|:|||||||||||||||||||||||||||||:|||||||||||.|.|||||||||.||||
Mouse   911 AALYRRQKQRKELSHYTASFAYTPAVSFPHSEGLSGSSSRPPMGLGPAAYPWLADSWPHPPRSPS 975

Human   975 AQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPGEGPVYSTIDPAGEELQTFHGGFPQH 1039
            |||||||||||||||||||||||||||||||||||..|.|||||||||||.||||||||||||||
Mouse   976 AQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGANASGEGPVYSTIDPVGEELQTFHGGFPQH 1040

Human  1040 PSGDLGPWSQYAPPEWSQGDSGAKGGKVKLLGKPVQMPSLNWPEALPPPPPSCELSCLEGPEEEL 1104
            .|||...||||||||||:|||||:||:.||||||||||||:||||||||||||||||.|||||||
Mouse  1041 SSGDPSTWSQYAPPEWSEGDSGARGGQGKLLGKPVQMPSLSWPEALPPPPPSCELSCPEGPEEEL 1105

Human  1105 EGSSEPEEWCPPMPERSHLTEPSSSGGCLVTPSRRETPSPTPSYGQQSTATLTPSPPDPPQPPTD 1169
            :|||:.||||||:||:|||...||||.|:|.|:.|:|||||.|||||||||||||||||||||||
Mouse  1106 KGSSDLEEWCPPVPEKSHLVGSSSSGACMVAPAPRDTPSPTSSYGQQSTATLTPSPPDPPQPPTD 1170

Human  1170 MPHLHQMPRRVPLGPSSPLSVSQPMLGIREARPAGLGAGPAASPHLSPSPAPSTASSAPGRTWQG 1234
            :|||||||||||||||||||||||.|...:.||.||||||..|.|.||||.|||||||||||.|.
Mouse  1171 IPHLHQMPRRVPLGPSSPLSVSQPALSSHDGRPVGLGAGPVLSYHASPSPVPSTASSAPGRTRQV 1235

Human  1235 NGEMTPPLQGPRARFRKKPKALPYRRENSPGDLPPPPLPPPEEEASWALELRAAGSMSSLERERS 1299
            .|||||||.|.|||.||||||||||||:||||||||||||||||.||.|.|||||||||||||||
Mouse  1236 TGEMTPPLHGHRARIRKKPKALPYRREHSPGDLPPPPLPPPEEETSWPLGLRAAGSMSSLERERS 1300

Human  1300 GERKAVQAV-----PLAAQRVLHPD--------EEAWLPYSRPSFLSRGQGTSTCSTAGSNSSRG 1351
            |||:.||||     ||.|||..|||        |||||||.||||||.|||||||||||||||||
Mouse  1301 GERRVVQAVPLGAQPLGAQRGPHPDAALLGCAAEEAWLPYGRPSFLSHGQGTSTCSTAGSNSSRG 1365

Human  1352 SSS---SRGSRGPGRSRSRSQSRSQSQRPGQKRREEPR 1386
            |:|   ||||||||||||||:|:|||||||:.||||||
Mouse  1366 SNSSRGSRGSRGPGRSRSRSRSQSQSQRPGRNRREEPR 1403

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ROBO3	NP_071765.2	Ig	64..162	CDD:299845	94/97 (97%)
		I-set	64..161	CDD:254352	93/96 (97%)
		I-set	168..249	CDD:254352	69/80 (86%)
		Ig	170..254	CDD:299845	70/83 (84%)
		I-set	258..343	CDD:254352	73/84 (87%)
		Ig	275..343	CDD:299845	57/67 (85%)
		I-set	347..441	CDD:254352	78/93 (84%)
		Ig	361..450	CDD:299845	77/88 (88%)
		I-set	451..531	CDD:254352	64/79 (81%)
		IGc2	465..528	CDD:197706	49/62 (79%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	541..563		15/21 (71%)
		FN3	556..649	CDD:238020	82/92 (89%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	639..662		19/23 (83%)
		FN3	680..760	CDD:238020	69/79 (87%)
		FN3	770..863	CDD:238020	80/92 (87%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	965..989		22/23 (96%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1028..1310		231/286 (81%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1327..1386		51/61 (84%)
Robo3	XP_006510170.1	Ig	64..162	CDD:386229	94/97 (97%)
		Ig	170..254	CDD:386229	70/83 (84%)
		Ig	275..343	CDD:386229	57/67 (85%)
		Ig4_Robo	361..450	CDD:143203	77/88 (88%)
		Ig	451..531	CDD:386229	64/79 (81%)
		FN3	564..649	CDD:238020	76/84 (90%)
		FN3	678..761	CDD:238020	71/82 (87%)
		FN3	771..864	CDD:238020	80/92 (87%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C83963205
Domainoid	1	1.000	754	1.000	Domainoid score	I3258
eggNOG	1	0.900	-	-		E1_KOG4222
HGNC	1	1.500	-	-
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H32119
Inparanoid	1	1.050	2281	1.000	Inparanoid score	I1164
Isobase	0	0.000	Not matched by this tool.
NCBI	1	1.000	-	-
OMA	1	1.010	-	-		QHG34979
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		oto117918
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R14406
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	16	16.190