Protein Alignment BLM and blm

DIOPT Version :10

• Sequence 1: NP_000048.1
• Gene: BLM / 641
• HGNCID: 1058
• Length: 1417
• Species: Homo sapiens
• Sequence 2: XP_017207538.1
• Gene: blm / 572540
• ZFINID: ZDB-GENE-070702-5
• Length: 1450
• Species: Danio rerio
• Alignment Length: 1550
• Identity: 731/1550 - (47%)
• Similarity: 933/1550 - (60%)
• Gaps: 248/1550 - (16%)

Human     1 MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNV--SVAKTPVLRNK 63
            |:::|||||:|.||||: ....|||||.|||..||.||||:||  .:|...|  .|..:.||.|:
Zfish    12 MSSLPQNNLKEHLERHN-NAAQNKLSLLKPKPGGFCFKKKSSS--GISKVEVPQKVTGSSVLANR 73

Human    64 DVNVTEDFSF------------------------SEPLPNTTNQQRVKDFFKNAPAGQETQRGGS 104
            .||:..:|..                        |:|..||.|...........|..|.|.:.
Zfish    74 SVNIPSNFEVTKNPVTFSQKPERVAPKVNFFTAPSKPKTNTVNPLANPFALNKTPLVQSTIKA-- 136

Human   105 KSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSF 169
               ||.....||:    :.|..||:...|  :.:|.|          |:|||:|||:|  ..||.
Zfish   137 ---LPKSAALPKD----STNDQTVELKHD--ISQLSF----------NEWDDLDDFET--PVKSR 180

Human   170 VTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEE-QKDDSEWLSS 233
            |..|    |..::.:|.....:|       |:::.       ||:.|:..:.|| |..::.....
Zfish   181 VASP----VAGTSTKKPSVSDQN-------TSSSC-------SSKCEETKVNEESQATETITAPK 227

Human   234 DVICI-------------DDGPIAEVHINEDAQESDSLKTHLEDER-------DNSEKK-KNLEE 277
            ||:..             :|.||.:....:.:.:..:|.:..|||.       |.::|. |::..
Zfish   228 DVLSAANGVSTETAEREPEDSPIKKSKRPKKSVQHTALLSDTEDEEIIHCVSPDTNQKNFKSVAV 292

Human   278 AELHSTEKVPCIEFDD----DDYD--TDFVPPS--PEEIISASSSSSKCLSTLKDLDTSDRKEDV 334
            ||.....:...|:.||    :.|:  .||:|||  ||||  :.|.|.|..|:.:.:..:::||.
Zfish   293 AEKDKWGEPNVIDSDDCEENNHYEGFEDFIPPSPIPEEI--SLSVSDKEKSSSEPVTPANKKES- 354

Human   335 LSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNE 399
             |.|...|..|...|.:.|.            .....|..|||.||.|:||||:.:|..|.||.|
Zfish   355 -SRSASGLPAPLDQSAKGLK------------GADDALFSVMESICCLVDTIPEHELIALTCGTE 406

Human   400 LLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVS 464
            ||.||..|:::|.:...:::..|                   ||..|...:....|...|||..|
Zfish   407 LLLQRAHRKRILAKGGSSRTSHS-------------------DSVSTPGFLNRPTFGVTPSNLTS 452

Human   465 PGDCLLTTTLG-----KTGFSATR----------KNLFE-------------------------- 488
                |...|.|     |||||..:          :::||
Zfish   453 ----LTPVTSGKREGVKTGFSFRKSIASVMSVGDESVFEDSDCIINGVETPGGTWNPNSSTKISA 513

Human   489 -RPLFNTHLQKSFVSSNWAETPRLGKK---NESS------YFPGNVLTSTAVKDQNK--HTASIN 541
             |..||..:|.  :|...::|.:...:   ||||      |.|..|.:.:...|.:.  :.|..:
Zfish   514 GRDTFNGSIQT--LSKPESKTDKCYSRLSFNESSNQTDLFYSPKRVDSGSRNADSSVEINIAGSS 576

Human   542 DLERETQPSYD--IDNFDIDDFDDDD--DWEDIMHNLAASKSSTAAYQP-IKEGRPIKSVSERLS 601
            .|....:|..|  :|:|||||||::|  |:.:...::..|::::....| ::||...||. ||
Zfish   577 SLRTGAEPVDDFLLDDFDIDDFDENDIPDYYEEPPSVLESRNNSGVKTPSVQEGGSSKSF-ER-- 638

Human   602 SAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLH 666
              ||...|...:.:..| .|.:.            ||..|:||:..:|||:.|||||||||||||
Zfish   639 --KTFTPPAPKSIKTPN-PEPLY------------RNPAHDRFRGFNFPHSPEMMKIFHKKFGLH 688

Human   667 NFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLD 731
            .||.||||||||:|||||.|:|||||||||||||||||||.|||||||||||||||||||||:||
Zfish   689 QFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLD 753

Human   732 IPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDE 796
            |.||.|:|||.||||..||:|||:|||.|||||.||||:|||.|:||.|:|||||.||||.||||
Zfish   754 ICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDE 818

Human   797 AHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHN 861
            ||||||||||||.||||::.||:.||:||:|||||||.||||||||.||.:.|||||:|||||:|
Zfish   819 AHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAMTRPQVFTMSFNRNN 883

Human   862 LKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSA 926
            |||.|||||||||..:|::||:|::|.||||:|||||.:|||:||:|||.|:||||||||||||.
Zfish   884 LKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSD 948

Human   927 RDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLL 991
            |:.||.||||||||||:||||||||||||||||:|||||||||||||||||||||||||||||:|
Zfish   949 REYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVL 1013

Human   992 FYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCK 1056
            ||:|.||.|:||||.|:||||..::.||.|||:||||:|||:.|||||||||||||:.||..|||
Zfish  1014 FYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCK 1078

Human  1057 KHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGS 1121
            :||:|.||||.:...||:|:||||||.|.||||::....|.|..|. ....|.|:||||:|||||
Zfish  1079 EHPEVICDNCARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKS-AQQNRLTLNMLVEIFLGS 1142

Human  1122 KSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLK 1186
            |||:||:|:||.|:|||:||||||||||:||.:|.|||||..:.||:||:..|.||.:||||.::
Zfish  1143 KSARIQTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQ 1207

Human  1187 VDFMETENSSSVKKQKALVA-KVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLA 1250
            |:|.|||::||::|.:|.|. |||:||||||||||||.::||.||||||:||:|||:|.||||:|
Zfish  1208 VEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGIHYYNIFSTATLKKIA 1272

Human  1251 ESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAE---DSSPGISLSSSRGPGRSAA 1312
            |:||:||||||||||||||||||||||.|.:|||||||..|||   :||..|  .::||......
Zfish  1273 ETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYSEWQLPAEAQAESSGWI--DTTRGHQNDED 1335

Human  1313 EELDEEI---PVSSHYFASKTRNERKRKKMPASQRSKRRKTASSG--SKAKGGSATCRKISSKTK 1372
            :..|::.   .|:|.||.|.:....|||:...|::.|||| .|||  |.||||      .||...
Zfish  1336 DNDDDDEGGGDVTSTYFRSNSGRGAKRKQGSYSRKPKRRK-GSSGQNSSAKGG------YSSNWS 1393

Human  1373 SSS--------IIGSSSASHTSQ-ATSGANSKL-GIMAPPKP--INRPFLKPSYA 1415
            ||.        ..||..|...|: |.||:.:|. |.|:.|.|  ..|||||||::
Zfish  1394 SSRGGGRGGGYRGGSRGAGRGSRSAPSGSAAKRPGFMSLPTPQAAARPFLKPSFS 1448

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
BLM	NP_000048.1	BLM_N	1..368	CDD:465064	115/422 (27%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..21		11/19 (58%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	203..227		6/24 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	250..291		10/48 (21%)
		Necessary for interaction with SPIDR. /evidence=ECO:0000269|PubMed:23509288	301..600		95/358 (27%)
		Necessary for dimerization and homooligomerization. /evidence=ECO:0000269|PubMed:28228481	362..414		22/51 (43%)
		BDHCT	372..412	CDD:462356	21/39 (54%)
		BDHCT_assoc	425..647	CDD:465065	64/279 (23%)
		recQ_fam	657..1143	CDD:129701	366/485 (75%)
		DEAH box	795..798		2/2 (100%)
		3' overhang DNA-binding. /evidence=ECO:0000269|PubMed:25901030	870..873		2/2 (100%)
		3' overhang DNA-binding. /evidence=ECO:0000269|PubMed:24816114, ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CGZ, ECO:0007744|PDB:4O3M	897..899		1/1 (100%)
		3' overhang DNA-binding. /evidence=ECO:0000269|PubMed:24816114, ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CGZ, ECO:0007744|PDB:4O3M	1000..1003		1/2 (50%)
		DNA Holliday junction binding. /evidence=ECO:0000269|PubMed:24257077	1094..1139		25/44 (57%)
		3' overhang DNA-binding. /evidence=ECO:0000269|PubMed:24816114, ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CGZ, ECO:0007744|PDB:4O3M	1110..1112		1/1 (100%)
		3' overhang DNA-binding. /evidence=ECO:0000269|PubMed:24816114, ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CGZ, ECO:0007744|PDB:4O3M	1121..1125		3/3 (100%)
		3' overhang DNA-binding. /evidence=ECO:0000269|PubMed:24816114, ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CGZ, ECO:0007744|PDB:4O3M	1160..1166		2/5 (40%)
		HRDC	1213..1292	CDD:128635	62/78 (79%)
		Necessary for ssDNA and DNA Holliday junction binding. /evidence=ECO:0000269|PubMed:20639533	1227..1244		12/16 (75%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1289..1417		53/146 (36%)
		Nuclear localization signal. /evidence=ECO:0000255	1334..1349		6/14 (43%)
blm	XP_017207538.1	BLM_N	12..346	CDD:465064	107/379 (28%)
		BDHCT	379..419	CDD:462356	21/39 (54%)
		recQ_fam	679..1164	CDD:129701	366/485 (75%)
		HRDC	1235..1314	CDD:128635	62/78 (79%)