DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment BLM and Blm

DIOPT Version :9

Sequence 1:	NP_000048.1	Gene:	BLM / 641	HGNCID:	1058	Length:	1417	Species:	Homo sapiens
Sequence 2:	NP_524319.2	Gene:	Blm / 41366	FlyBaseID:	FBgn0002906	Length:	1487	Species:	Drosophila melanogaster

Alignment Length:	1536	Identity:	466/1536 - (30%)
Similarity:	715/1536 - (46%)	Gaps:	299/1536 - (19%)

- Green bases have known domain annotations that are detailed below.

Human    12 QLERHSARTLNNK--LSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAK-----TPVLRNKDVNVTE 69
            :|::|:...|:..  ..||..|::..:.||.||.|.:||.....:||     :|   .|.|....
  Fly    82 KLKKHTYLDLSVSPLAKLSAKKYARDSPKKPTSLDLSVSPLAELLAKKSDRDSP---KKPVQNEN 143

Human    70 DFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDT 134
            .:::.....:....:.:.|..:..|   :.:|..|...|.|   :|::.|  |||.   :|..|:
  Fly   144 SYTYRGLSESPVENKSIGDTLRKPP---QKERKTSIVWLSD---SPEKKV--TQNE---RKILDS 197

Human   135 ALKKLEFSSSPDS-------LSTIND---------------WDDMDDFDTSETSKSFVTP-PQSH 176
            .|::..|...|:.       |.|:.|               |  .::..|.:...|...| ..::
  Fly   198 PLQRFSFEDFPNKENGNRHHLLTLPDSPPPPQPVKKPEKTMW--QNETKTIQDKDSPANPLVSNN 260

Human   177 FVRVST------AQKSKKG-KRNFFKAQLYTTNTVKTDLPPPSSESEQ---IDLTEEQ----KDD 227
            ...:||      |..:.|| .||.|:           |.|..|...||   :...:|.    |..
  Fly   261 LASISTLLDSSRAPNTYKGSSRNLFE-----------DSPEKSGSGEQGYKLGSAKENEIPTKPA 314

Human   228 SEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLED--ERDNSEKKKNLEEAE-LHST------ 283
            :..|..:.:.....|.|.:.........:||..:|:|  :.||.....|.:..| |.||
  Fly   315 TASLERNSVTSSPSPAAPLKPRYSVAFDNSLADYLKDLAQNDNFSIDPNKQNTETLKSTLGFFRN 379

Human   284 -------------EKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTS-DRKEDV 334
                         :::|.:.|:      :.....|...:.......|..:..:.:..| |:||..
  Fly   380 TYVELMEKYCSLIDQIPAMHFN------EIAGFQPNTFLKLKVMRQKFKARTQLVQNSLDKKESQ 438

Human   335 LSTSKDLLSKPEKMSMQ----------ELNPETS---------TDCDARQISLQQQLIHVMEHIC 380
            |...::.|.| |::.||          ..:||.|         .:....:|..:.||||   .:|
  Fly   439 LKAEQEALEK-EEIEMQAEQAQQTVLSSSSPEKSRPIMPLPKVQEIKDEKIPNRNQLIH---DLC 499

Human   381 KLIDTI-----PDDKLKLLDCGNELLQQRNIRRKLLTEV-----DFN----KSDASLLGSLWRY- 430
            ...|..     |.|        .:|:.:   |::|:.::     ||:    ::|..||......
  Fly   500 GEPDNFSPPSSPRD--------TQLIPK---RQQLINDLCGEPDDFSPPSKQNDPHLLRKCEELV 553

Human   431 -----RPDS-------LDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATR 483
                 .||.       |||.:|.:         :||           |....|||.|........
  Fly   554 HDLCEEPDDYLAQSMMLDGDLEEE---------QLN-----------GPTQGTTTSGMDDDEDDL 598

Human   484 KNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQ 548
            :.|... :.:.|.:..            |:::|.:.:....|.:..||:::|.|.....|:.:..
  Fly   599 EGLLAE-IEDEHQKMQ------------GRRSEFNGYSYKELEAVKVKEKHKETPINISLDDDGF 650

Human   549 PSYDIDNFDIDDFDDDDDWEDIMHNLAA---SKSSTAAYQPIKEGRPIKSV----SERLSSAKTD 606
            |.||...|            :.||:.||   |:.|:|.....|...|.|..    |::||.
  Fly   651 PEYDEAMF------------EQMHSQAAANKSRVSSAGPSTSKSVVPTKQTSALHSQKLSG---- 699

Human   607 CLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTN 671
                       ||..::.|      ..:..      .|....|.|:..:|......|||.:||.|
  Fly   700 -----------NFHANVHN------DGITG------EFDGQKFEHSTRLMHGLSYSFGLKSFRPN 741

Human   672 QLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATY 736
            ||:.|||.|||.|||:||||||||||||||||.::.|||:|||||:|||.||:.||.||||.|..
  Fly   742 QLQVINATLLGNDCFVLMPTGGGKSLCYQLPAILTEGVTIVISPLKSLIFDQINKLASLDICAKS 806

Human   737 LTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVS 801
            |:|::..::...||..|..:.|::||||||||||.:|.|...||:.|.....::|||||||||||
  Fly   807 LSGEQKMADVMAIYRDLESQPPMVKLLYVTPEKISSSARFQDTLDTLNSNNYISRFVIDEAHCVS 871

Human   802 QWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYV 866
            |||||||.|||::.:|:::||:||.:||||||.|||:.|||.||.:...:.|..||||.||:|.|
  Fly   872 QWGHDFRPDYKKLGVLKKRFPNVPTIALTATATPRVRLDILAQLNLKNCKWFLSSFNRSNLRYRV 936

Human   867 LPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQ 931
            ||||......|...:||....:.|||||||||:|||..:..:.:||:.|::|||||:|:.|:..|
  Fly   937 LPKKGVSTLDDISRYIRSKPQHFSGIIYCLSRKECDETSKKMCKDGVRAVSYHAGLTDTDRESRQ 1001

Human   932 QKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYH 996
            :.|:. ...:|||||:||||||||||||||:|.|||||:||||||:|||||||:::.|:|:|.|.
  Fly  1002 KDWLT-GKMRVICATVAFGMGIDKPDVRFVLHYSLPKSIEGYYQEAGRAGRDGDVADCILYYNYS 1065

Human   997 DVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDV 1061
            |:.|:|:::..:|...::.::.|.:|||.:|.||||:|:|||.|.|.||||: |..:.|.::.:.
  Fly  1066 DMLRIKKMLDSDKALQYNVKKIHVDNLYRIVGYCENLTDCRRAQQLDYFGEH-FTSEQCLENRET 1129

Human  1062 SCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKI 1126
            :||||...:.||..|..:..:...|.|::..|.:           .|||:..:.|:..|||..||
  Fly  1130 ACDNCINKRAYKAVDALEHARKAARAVKDLCSGR-----------SRFTLLHIADVLKGSKIKKI 1183

Human  1127 ------QSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNL 1185
                  ::...|....:.:::..||.:|:::|..|.||| |..||...||:.|||....::.|..
  Fly  1184 IDFNHHKTPHHGVLKDWDKNDVHRLLRKMVIDGFLREDL-IFTNDFPQAYLYLGNNISKLMEGTP 1247

Human  1186 KVDFMETENSSSVKKQKALVAKVSQ------------REEMVKKCLGELTEVCKSLGKVFGVHYF 1238
            ..:|..|:|:   |:.||.|..||.            ..|:.::|..:|.::|:::.....|...
  Fly  1248 NFEFAVTKNA---KEAKAAVGSVSDGATSSTADGQSGMREIHERCYTDLLDLCRTIASQRNVTMA 1309

Human  1239 NIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSE-------WTSPAEDSS 1296
            :|.|...||.:||:|....:.:..|..||:...:||||:::.:...|:.       .....|:.:
  Fly  1310 SIMNIQALKSMAETLPITEKDMCSIPHVTKANFDKYGAKLLEITSNYASEKLLMQAVLDEEEEQA 1374

Human  1297 PGISLSSSRGPGRSAAEELDEEIPVSSHYFAS-------KTRNERKRKKMPASQRSKRRKTASSG 1354
            ......|:.|...   |.:|.::.|:|...|:       .:....||||:..|..|||.||:::
  Fly  1375 AAKQRPSTSGWNN---ESVDWDMAVASQGNANTSGASGFNSFRAGKRKKIYKSGASKRYKTSTT- 1435

Human  1355 SKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSK 1395
                  |...||.:|........|:..|..::.:.||..||
  Fly  1436 ------SPAARKTTSARGRGGRAGAKRAESSASSASGWKSK 1470

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
BLM	NP_000048.1	Necessary for dimerization and homooligomerization. /evidence=ECO:0000269\|PubMed:28228481	362..414		12/56 (21%)
		BDHCT	372..409	CDD:285334	9/41 (22%)
		BDHCT_assoc	425..647	CDD:292821	44/241 (18%)
		recQ_fam	657..1143	CDD:129701	241/491 (49%)
		DEAD	669..841	CDD:278688	105/171 (61%)
		DEAH box	795..798		2/2 (100%)
		HELICc	861..992	CDD:238034	74/130 (57%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:25901030	870..873		1/2 (50%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	897..899		1/1 (100%)
		RecQ_Zn_bind	997..1066	CDD:292742	27/68 (40%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	1000..1003		1/2 (50%)
		RQC	1072..1189	CDD:286470	33/122 (27%)
		DNA Holliday junction binding. /evidence=ECO:0000269\|PubMed:24257077	1094..1139		10/50 (20%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	1110..1112		1/1 (100%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	1121..1125		2/3 (67%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	1160..1166		2/5 (40%)
		HRDC	1213..1292	CDD:128635	20/85 (24%)
		Necessary for ssDNA and DNA Holliday junction binding. /evidence=ECO:0000269\|PubMed:20639533	1227..1244		3/16 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1289..1417		28/114 (25%)
		Nuclear localization signal. /evidence=ECO:0000255	1334..1349		8/14 (57%)
		BLM_N	1..366	CDD:292819	87/439 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21		2/8 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	203..227		7/30 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	250..291		12/62 (19%)
		Necessary for interaction with SPIDR. /evidence=ECO:0000269\|PubMed:23509288	301..600		70/352 (20%)
Blm	NP_524319.2	RQC	1140..1253	CDD:286470	34/124 (27%)
		HRDC	1286..1352	CDD:278967	18/65 (28%)
		RecQ	729..1359	CDD:223588	287/646 (44%)
		DEAD	739..908	CDD:278688	104/168 (62%)
		HELICc	931..1061	CDD:238034	74/130 (57%)
		RecQ_Zn_bind	1066..1134	CDD:292742	27/68 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165159433
Domainoid	1	1.000	216	1.000	Domainoid score	I2701
eggNOG	1	0.900	-	-		E1_COG0514
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	571	1.000	Inparanoid score	I1058
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D360651at33208
OrthoFinder	1	1.000	-	-		FOG0001239
OrthoInspector	1	1.000	-	-		oto90869
orthoMCL	1	0.900	-	-		OOG6_100352
Panther	1	1.100	-	-	LDO	PTHR13710
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2611
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.760