DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment BLM and RecQ5

DIOPT Version :9

Sequence 1:	NP_000048.1	Gene:	BLM / 641	HGNCID:	1058	Length:	1417	Species:	Homo sapiens
Sequence 2:	NP_729983.1	Gene:	RecQ5 / 39594	FlyBaseID:	FBgn0027375	Length:	1058	Species:	Drosophila melanogaster

Alignment Length:	898	Identity:	263/898 - (29%)
Similarity:	394/898 - (43%)	Gaps:	194/898 - (21%)

- Green bases have known domain annotations that are detailed below.

Human   651 HTKEMMKIFHKKFGLHNFRTN-QLEAINAAL-LGEDCFILMPTGGGKSLCYQLPACVSPG-VTVV 712
            |...:.:...|.||...|::: |.:|:..|: ..:|.::.||||.|||||:|||..:|.. :|:|
  Fly     3 HESAVHEALKKHFGHSKFKSDLQEKAVKCAVKKKQDVYVSMPTGSGKSLCFQLPGLMSENQITIV 67

Human   713 ISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLI 777
            .|||.:||.||:..||.|.:||..|....:..|...:.:.|......:|.||:|||: .|:....
  Fly    68 FSPLLALIKDQIDHLTKLKVPADSLNSKMSTKERDRVIMDLKAVRTNLKFLYITPEQ-AATKFFQ 131

Human   778 STLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDIL 842
            ..|:.|::...||.|.:||||||||||||||.||.::..||.|:..|..:||||||:..|::||.
  Fly   132 DLLQTLHKHNKLAYFAVDEAHCVSQWGHDFRPDYLKLGELRSKYSDVIWLALTATASREVKEDIY 196

Human   843 TQLKILRPQV-FSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKH---HPYD----------SGII 893
            .||::.:|.. ||....|.||.|.::.|...:..|..|....:|   :|.:          .||:
  Fly   197 KQLRLHQPVAQFSTPSFRKNLFYDIVYKNSIEDDFQHLADFARHCLGNPKEFKDTPKPQRGCGIV 261

Human   894 YCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDV 958
            ||.:|.:.:.||..:.:.|:.|:||||||....|.|||:.|:..|. .:||||.:||||:|||.|
  Fly   262 YCRTRDQVERMAIGVTKQGIGAVAYHAGLKTGERTEVQEAWMRGDQ-PIICATNSFGMGVDKPSV 325

Human   959 RFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTR------- 1016
            |||||..:|::|..||||||||||||..|:|.|:|...||..::  .:::.|. |..|
  Fly   326 RFVIHWDVPQNVAAYYQESGRAGRDGLQSYCRLYYGREDVRSIR--FLLQNDA-HRARGRGDKEL 387

Human  1017 --ETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVS--CDNC----------- 1066
              |........:..:||. |.||......:||:         ..||.|  ||.|
  Fly   388 LTERAIKQFEKITEFCER-TTCRHKLFSDFFGD---------PTPDCSGQCDVCKRPKKAEKALE 442

Human  1067 -----CKTKDYKT----RDVTDDVK----SIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIF 1118
                 |....:|:    :|..|..:    .|.|..||::..:...:    ..||:...:|
  Fly   443 IFHRLCMDDAFKSHISLQDCADLYEGGRPGIKRAAQEYAGGESGSD----DDSGQSHSSM----- 498

Human  1119 LGSKSAKIQSGIFGKGSAYSRHN--------------------AERLFKKLILDKILDEDLYINA 1163
              :|.||.:|..|.|.....|..                    ||...||:...:....:.|:.|
  Fly   499 --AKRAKKESQDFIKQQFNLRKQISAARQLEQETIAQISRVRMAEATEKKIAGLQATHREKYLTA 561

Human  1164 NDQAIAYVM------LGNKAQTVL--------NGNLKVDFMETENSSSVKKQKALVAKVSQREEM 1214
            ...|:...:      .|.:.::||        :.|::.|... :|..:...:.|||.::|    .
  Fly   562 LIDALKANVDKCKDEPGQQPKSVLKYNDYEAMSVNMEYDVFR-QNKVANMYRHALVKEIS----T 621

Human  1215 VKKCLGELTEVCK---------------SLGKVFG--VHYFNIFNTVTLKKLAESLSSDPEVLLQ 1262
            :||    |||..|               |.|...|  |.||.       :||.|.....|:.|.:
  Fly   622 IKK----LTEQTKLLPLLVDYIPKPETSSKGAWTGGSVAYFE-------RKLKELEEQRPQSLKE 675

Human  1263 IDGVTEDK--LEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIP---VS 1322
            :....:|:  .::..::..|:...:.:........||   :...|...:|...:|:|..|   .|
  Fly   676 VPKALKDRKGFKQENSKQTSISSFFKKEIKEEPIDSP---IQEERVNIKSEQMDLEESTPKDIAS 737

Human  1323 SHY--------FASKTR----------NERKRKKM---PASQRSKRRKTASSGSKAKGGSATCRK 1366
            :|.        .:|..|          .|||.|.|   ..|..:.|||..|..::.|      .|
  Fly   738 NHVKEESVTPESSSSDRELELQPDSIIGERKFKLMSNGDGSYDTHRRKRVSHETQPK------EK 796

Human  1367 ISSKTKSS--SIIGS--SSASHTSQATSG----------ANSKLGIMAPPKPI 1405
            .|.|||||  .:.||  |.:..::|:.:|          .||||..:....||
  Fly   797 ESIKTKSSMQHLFGSFKSESEESNQSLNGFKTARQMLEENNSKLEGVPEKLPI 849

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
BLM	NP_000048.1	Necessary for dimerization and homooligomerization. /evidence=ECO:0000269\|PubMed:28228481	362..414
		BDHCT	372..409	CDD:285334
		BDHCT_assoc	425..647	CDD:292821
		recQ_fam	657..1143	CDD:129701	186/557 (33%)
		DEAD	669..841	CDD:278688	73/174 (42%)
		DEAH box	795..798		2/2 (100%)
		HELICc	861..992	CDD:238034	62/143 (43%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:25901030	870..873		0/2 (0%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	897..899		0/1 (0%)
		RecQ_Zn_bind	997..1066	CDD:292742	18/79 (23%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	1000..1003		0/2 (0%)
		RQC	1072..1189	CDD:286470	28/158 (18%)
		DNA Holliday junction binding. /evidence=ECO:0000269\|PubMed:24257077	1094..1139		9/44 (20%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	1110..1112		0/1 (0%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	1121..1125		1/3 (33%)
		3' overhang DNA-binding. /evidence=ECO:0000269\|PubMed:24816114, ECO:0000269\|PubMed:25901030, ECO:0007744\|PDB:4CGZ, ECO:0007744\|PDB:4O3M	1160..1166		2/5 (40%)
		HRDC	1213..1292	CDD:128635	19/97 (20%)
		Necessary for ssDNA and DNA Holliday junction binding. /evidence=ECO:0000269\|PubMed:20639533	1227..1244		7/33 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1289..1417		40/155 (26%)
		Nuclear localization signal. /evidence=ECO:0000255	1334..1349		6/17 (35%)
		BLM_N	1..366	CDD:292819
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	203..227
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	250..291
		Necessary for interaction with SPIDR. /evidence=ECO:0000269\|PubMed:23509288	301..600
RecQ5	NP_729983.1	RecQ	6..>442	CDD:223588	168/450 (37%)
		DEAD	37..193	CDD:278688	70/156 (45%)
		HELICc	<257..359	CDD:238034	54/102 (53%)
		RecQ_Zn_bind	364..431	CDD:292742	18/79 (23%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0514
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D445763at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.820