DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ROBO1 and robo2

DIOPT Version :9

Sequence 1:	XP_011532278.1	Gene:	ROBO1 / 6091	HGNCID:	10249	Length:	1654	Species:	Homo sapiens
Sequence 2:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster

Alignment Length:	1503	Identity:	426/1503 - (28%)
Similarity:	664/1503 - (44%)	Gaps:	280/1503 - (18%)

- Green bases have known domain annotations that are detailed below.

Human    52 NSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPR 116
            |.|...|: |:.|:  |||:|||.|..|.|.:|.|.||:|||.|||||:|:|.|..::||..
  Fly    78 NGLTQVGA-LKGEN--PRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELKTDTG--- 136

Human   117 SHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMV 181
            |||::||:|.||||:::|.|: ..|.|.|.|.|:|..|.|.|.||:|:||.|||:||..|::..|
  Fly   137 SHRIMLPAGGLFFLKVIHSRR-ESDAGTYWCEAKNEFGVARSRNATLQVAFLRDEFRLEPANTRV 200

Human   182 AVGEPAVMECQPPRGHPEPTISWKKDGSPLD---DKDERITIRGGKLMITYTRKSDAGKYVCVGT 243
            |.||.|:|||..|||.|||.|||:|:|..|:   :|..|| :.||.|.|...|:||.|:|.||..
  Fly   201 AQGEVALMECGAPRGSPEPQISWRKNGQTLNLVGNKRIRI-VDGGNLAIQEARQSDDGRYQCVVK 264

Human   244 NMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRK--DDGELPKSR 306
            |:||.|||..|.|.|..||..::.|.|....|..|..|:|...|||:|.|.||:  ..|.:|..|
  Fly   265 NVVGTRESATAFLKVHVRPFLIRGPQNQTAVVGSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRR 329

Human   307 YEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTV 371
            ..:.:|.:||:..||..|||.|||.|:|.||...|:..|||..   ||.||::|::|:|.:|..|
  Fly   330 VHVLEDRSLKLDDVTLEDMGEYTCEADNAVGGITATGILTVHA---PPKFVIRPKNQLVEIGDEV 391

Human   372 TFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGD----LTITNVQRSDVGYYI-C 431
            .|:|:|.|:|:|.::|..||:.:||.   |.....|..|:.|.:    |:|....|.|.|..: |
  Fly   392 LFECQANGHPRPTLYWSVEGNSSLLL---PGYRDGRMEVTLTPEGRSVLSIARFAREDSGKVVTC 453

Human   432 QTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGV 496
            ..||..||:.::..:.| |...:.|||:|.|||||||:.|....||.|...|:|||.:.|..||:
  Fly   454 NALNAVGSVSSRTVVSV-DTQFELPPPIIEQGPVNQTLPVKSIVVLPCRTLGTPVPQVSWYLDGI 517

Human   497 LVSTQDSRIKQLEN-GVLQIR-YAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTD 559
            .:..|:...:.|.: |.|.|. ..:..|.|.|||:||..:|:::||.|:.:.         .||:
  Fly   518 PIDVQEHERRNLSDAGALTISDLQRHEDEGLYTCVASNRNGKSSWSGYLRLD---------TPTN 573

Human   560 PNL----------IPSAPSKPEVTDVSRNTVTLSW-QPNLNSGATPTSYIIEAFSHASGSSWQTV 613
            ||:          .|..|.||::.:...|:||||| :.|...|::...|:||.|.......|..|
  Fly   574 PNIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAV 638

Human   614 AENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRE-LG 677
            ...|:..|....||.|...|.||:||.|::|:|.||.:|:|: |......:.|:|..:.:.. |.
  Fly   639 GTRVQNTTFTQTGLLPGVNYFFLIRAENSHGLSLPSPMSEPI-TVGTRYFNSGLDLSEARASLLS 702

Human   678 NAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYR--------------------------- 715
            ..|:.|.|.:|:.|:|:::.|.: ...:|::|:.:..|
  Fly   703 GDVVELSNASVVDSTSMKLTWQI-INGKYVEGFYVYARQLPNPIVNNPAPVTSNTNPLLGSTSTS 766

Human   716 ------------------PSGANHGESDWLVFEVRTP--------------AKNSVVIPDLRKGV 748
                              .:|...||::.......||              ..:|..|..|.:..
  Fly   767 ASASASASALISTKPNIAAAGKRDGETNQSGGGAPTPLNTKYRMLTILNGGGASSCTITGLVQYT 831

Human   749 NYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMV 813
            .||....||:...:|..|..:.|:|||:.||..|.|:.....  |.:|:.:.|:.|....::|::
  Fly   832 LYEFFIVPFYKSVEGKPSNSRIARTLEDVPSEAPYGMEALLL--NSSAVFLKWKAPELKDRHGVL 894

Human   814 QEYKVWCLGNETRYHI-----NKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQ 873
            ..|.|...|.:|.::.     |.|:|.::.::|:..|..|:.|:|.|||...||.|....|..::
  Fly   895 LNYHVIVRGIDTAHNFSRILTNVTIDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLR 959

Human   874 LDAHGNPVSP--EDQVSLAQ-QISDVVKQPAFIAGIGAACWIILMVFSIWLY---RHRKKRNGLT 932
            ||.....:.|  ..:..:.| .::||:.||.||..:||...::::.|...::   :|...:....
  Fly   960 LDPITKRLDPFINQRYPINQDHVNDVLTQPWFIILLGAILAVLMLSFGAMVFVKRKHMMMKQSAL 1024

Human   933 STYAG-----IRKVPSFTFTPTVTYQRGGEAV----SSGG---RPGLLNISEPAAQPWLADTWPN 985
            :|..|     :.|:||.:       .|.|...    |:||   ||.....|....:..:||..|.
  Fly  1025 NTMRGNHTSDVLKMPSLS-------ARNGNGYWLDSSTGGMVWRPSPGGDSLEMQKDHIADYAPV 1082

Human   986 TGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDV-DLSNK 1049
            .|...:.......:.|:|.:.|.    :...|.|...:....|:|..:        |:. ::...
  Fly  1083 CGAPGSPAGGGTSSGGSGGAGSG----ASGGDDIHGGHGSERNQQRYV--------GEYSNIPTD 1135

Human  1050 INEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNN---------GSGDSGEKHWKPL 1105
            ..|:.:|.....:.||..|.|  |.||||:.::..:.......         |.|.....|  |.
  Fly  1136 YAEVSSFGKAPSEYGRHGNAS--PAPYATSSILSPHQQQQQQQPRYQQRPVPGYGLQRPMH--PH 1196

Human  1106 GQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQS------YDQNTGGS--------YNSS 1156
            .||:|.   .|....:.::.::..:.:..:||:..|.|.      |..|||.|        |...
  Fly  1197 YQQQQH---QQQQAQQTHQQHQALQQHQQLPPSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYPK 1258

Human  1157 D------------------------RGSSTSGSQ---------------GHKKGARTPKVPKQGG 1182
            |                        :.||...||               | ::.||...:....|
  Fly  1259 DKQRHIHITENKLSNCHTYEAAPGAKQSSPISSQFASVRRQQLPPNCSIG-RESARFKVLNTDQG 1322

Human  1183 MNWADLL--------------------PPPPAHPPPHSNSEE-YNISVDESYDQEMPCPVPPARM 1226
            .|..:||                    |.|.|....|.:... |:.:..:..|.|        |:
  Fly  1323 KNQQNLLDLDGSSMCYNGLADSGCGGSPSPMAMLMSHEDEHALYHTADGDLDDME--------RL 1379

Human  1227 YLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQ 1291
            |::.||.:..:.::...|.|....:.....|:.:|||.:...:.||        |.:|...:.:.
  Fly  1380 YVKVDEQQPPQQQQQLIPLVPQHPAEGHLQSWRNQSTRSSRKNGQE--------CIKEPSELIYA 1436

Human  1292 P----------DRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKM-- 1344
            |          .......|..|....: |     .:.|..:....:.:..:.:.::|::..:.
  Fly  1437 PGSVASERSLLSNSGSGTSSQPAGHNVXP-----LLPGSSLGQSQSKSQSQSQSQSDLQSQEQLY 1496

Human  1345 --QTRRLL 1350
              :.|.||
  Fly  1497 AREVRELL 1504

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ROBO1	XP_011532278.1	Ig1_Robo	67..166	CDD:143317	50/98 (51%)
		I-set	68..165	CDD:254352	50/96 (52%)
		I-set	172..258	CDD:254352	45/88 (51%)
		Ig2_Robo	174..258	CDD:143201	43/86 (50%)
		I-set	262..347	CDD:254352	34/86 (40%)
		Ig3_Robo	279..347	CDD:143202	29/69 (42%)
		I-set	354..448	CDD:254352	33/98 (34%)
		Ig4_Robo	368..457	CDD:143203	29/93 (31%)
		I-set	458..545	CDD:254352	36/88 (41%)
		Ig	462..536	CDD:299845	30/75 (40%)
		FN3	564..650	CDD:238020	32/86 (37%)
		FN3	687..773	CDD:238020	22/144 (15%)
		fn3	781..867	CDD:278470	25/90 (28%)
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	50/100 (50%)
		I-set	91..184	CDD:254352	50/96 (52%)
		I-set	190..279	CDD:254352	45/89 (51%)
		Ig2_Robo	193..279	CDD:143201	43/86 (50%)
		I-set	283..370	CDD:254352	34/86 (40%)
		Ig	300..370	CDD:299845	29/69 (42%)
		Ig	388..>457	CDD:299845	23/71 (32%)
		I-set	479..561	CDD:254352	33/81 (41%)
		Ig	496..561	CDD:143165	23/64 (36%)
		FN3	588..681	CDD:238020	35/93 (38%)
		FN3	<817..856	CDD:238020	10/38 (26%)
		fn3	864..952	CDD:278470	25/89 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6690
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	9	8.770