DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ROBO1 and robo1

DIOPT Version :9

Sequence 1:	XP_011532278.1	Gene:	ROBO1 / 6091	HGNCID:	10249	Length:	1654	Species:	Homo sapiens
Sequence 2:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster

Alignment Length:	1645	Identity:	462/1645 - (28%)
Similarity:	671/1645 - (40%)	Gaps:	434/1645 - (26%)

- Green bases have known domain annotations that are detailed below.

Human    68 PRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRI 132
            |||:|||:||:|.|.||||||||.||:|.|||||:|.||.|.|  ::.:|||:....|:|||.|.
  Fly    56 PRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVST--NEKKSHRVQFKDGALFFYRT 118

Human   133 VHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGH 197
            :.|:|.: |.|.|.|||:|.:|:|||.:|||::|:||||||..|.|..||.||.|::||.||:|.
  Fly   119 MQGKKEQ-DGGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGI 182

Human   198 PEPTISWKKDGSPLDD-------KDERITI-RGGKLMITYTRKSDAGKYVCVGTNMVGERESEVA 254
            ||||:.|.|||.||||       ...|:.| .||.|:|:.....|.|.|.|:..|:||.|||..|
  Fly   183 PEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYA 247

Human   255 ELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEI-RDDHTLKIR 318
            :|.|..:|.|:|.|.:..:....:|.|.|...|||.|.|.|:|::|.:|.||..| .|:.:|:|.
  Fly   248 KLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKKEEGNIPVSRARILHDEKSLEIS 312

Human   319 KVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQP 383
            .:|..|.|:|.|.|.|.||:..|.|:|.|..   ||:|..:|.::.|.|...|...|.|:|||.|
  Fly   313 NITPTDEGTYVCEAHNNVGQISARASLIVHA---PPNFTKRPSNKKVGLNGVVQLPCMASGNPPP 374

Human   384 AIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEV 448
            ::||.:||...|:|   |..|..|..|:..|.|.||:|::.|.|||:|...:|..|...:.:|:|
  Fly   375 SVFWTKEGVSTLMF---PNSSHGRQHVAADGTLQITDVRQEDEGYYVCSAFSVVDSSTVRVFLQV 436

Human   449 TDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVL 513
            :. :.:||||:|:.||.|||:.......|.|.|||:|.|.|.|..||..|.. .:|...::...|
  Fly   437 SS-LDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDGHAVQA-GNRYSIIQGSSL 499

Human   514 QIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRN 578
            ::...:|.|:|.|||.||...||.:|:|.:.|::.| .....|..||:..|:.|..|:|.:|||.
  Fly   500 RVDDLQLSDSGTYTCTASGERGETSWAATLTVEKPG-STSLHRAADPSTYPAPPGTPKVLNVSRT 563

Human   579 TVTLSWQPNLNS-GATP--TSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAA 640
            :::|.|..:... ||..  ..|.:|.||....:.|...|:.|......|.||.|...|:|||||.
  Fly   564 SISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAE 628

Human   641 NAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQREL--GNAVLHLHNPTVLSSSSIEVHWT--VD 701
            |..|||.||.:|:.:||.:....:...:.....|.|  |.:| .|.:.:.:::|::.:.|.  |.
  Fly   629 NTQGISVPSGLSNTIKTIEADFDAASANDLSAARTLLTGKSV-ELIDASAINASAVRLEWMLHVS 692

Human   702 QQSQYIQGYKILYR----PSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQ 762
            ...:|::|.:|.|:    ||...|.      ..|...:..|.|:.:|:|...||....|||...:
  Fly   693 ADEKYVEGLRIHYKDASVPSAQYHS------ITVMDASAESFVVGNLKKYTKYEFFLTPFFETIE 751

Human   763 GADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKV-WCLGNETR 826
            |..|..|.|.|.|:.|||||..:.:...  |.||..|.|.|||....||.:..||: ...||..:
  Fly   752 GQPSNSKTALTYEDVPSAPPDNIQIGMY--NQTAGWVRWTPPPSQHHNGNLYGYKIEVSAGNTMK 814

Human   827 YHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLD---------AH----- 877
            ...|.|::.:|.||::..|..|..|||.:.:.|.||.|..|:|..:.:|         ||
  Fly   815 VLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHPPRAHPSGTH 879

Human   878 --------------GNPVSPED-QVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWL------ 921
                          || :.|.| ..:..::.:|.:..|          |::::|..:.|
  Fly   880 DGRHEGQDLTYHNNGN-IPPGDINPTTHKKTTDYLSGP----------WLMVLVCIVLLVLVISA 933

Human   922 -----YRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLAD 981
                 |..||.:                     :|.:.|..:|.|......|||:...:. |:
  Fly   934 AISMVYFKRKHQ---------------------MTKELGHLSVVSDNEITALNINSKESL-WI-- 974

Human   982 TWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDL 1046
                  ::|.....:.....:|.|:|.|.::...:.  :||||.          ...|.|.:||.
  Fly   975 ------DHHRGWRTADTDKDSGLSESKLLSHVNSSQ--SNYNNS----------DGGTDYAEVDT 1021

Human  1047 SNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQE 1111
            .|.........||:           .|||||||.:|.::.|......:..|.:|
  Fly  1022 RNLTTFYNCRKSPD-----------NPTPYATTMIIGTSSSETCTKTTSISADK----------- 1064

Human  1112 VAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPK 1176
                                              |..|...|:.:..|             :.|.
  Fly  1065 ----------------------------------DSGTHSPYSDAFAG-------------QVPA 1082

Human  1177 VPKQGG---------MNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDE 1232
            ||....         :||::.|||||.||||.|.         ..|.|..|
  Fly  1083 VPVVKSNYLQYPVEPINWSEFLPPPPEHPPPSST---------YGYAQGSP-------------- 1124

Human  1233 LEEEEDERGPTPPVRGAASSPAAVSYS-HQST----ATLTPSPQEELQPMLQDCPEETGHMQHQP 1292
               |...:.......|.:::.:.::.| |.|:    :....|||..:.     ||.|  ::...|
  Fly  1125 ---ESSRKSSKSAGSGISTNQSILNASIHSSSSGGFSAWGVSPQYAVA-----CPPE--NVYSNP 1179

Human  1293 --------DRRRQ--PVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTR 1347
                    ..|.|  |.:..||..|     .|...:||
  Fly  1180 LSAVAGGTQNRYQITPTNQHPPQLP-----AYFATTGP--------------------------- 1212

Human  1348 RLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAA 1412
                                          |.|...:::                  .||....|
  Fly  1213 ------------------------------GGAVPPNHL------------------PFATQRHA 1229

Human  1413 AAEY-AGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRR 1476
            |:|| |||..||                |.:..:..|.|:..:.:.||
  Fly  1230 ASEYQAGLNAAR----------------CAQSRACNSCDALATPSPMQ----------------- 1261

Human  1477 ETYTDDLPPPPVP-PPAIKSPTAQSKTQLEVRPVVVPKLPSMDAR-----TDRSSDRKGSSYKGR 1535
                   |||||| |.....|         |.|...|..|:....     :...||...||...:
  Fly  1262 -------PPPPVPVPEGWYQP---------VHPNSHPMHPTSSNHQIYQCSSECSDHSRSSQSHK 1310

Human  1536 EVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSN- 1599
            ..|                :.:|..:..|.||....:|              .|..:|...:.|
  Fly  1311 RQL----------------QLEEHGSSAKQRGGHHRRR--------------APVVQPCMESENE 1345

Human  1600 ------NPRDPSSSSSMSSR 1613
                  ..|..:|....|||
  Fly  1346 NMLAEYEQRQYTSDCCNSSR 1365

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ROBO1	XP_011532278.1	Ig1_Robo	67..166	CDD:143317	55/97 (57%)
		I-set	68..165	CDD:254352	55/96 (57%)
		I-set	172..258	CDD:254352	44/93 (47%)
		Ig2_Robo	174..258	CDD:143201	42/91 (46%)
		I-set	262..347	CDD:254352	34/85 (40%)
		Ig3_Robo	279..347	CDD:143202	30/68 (44%)
		I-set	354..448	CDD:254352	35/93 (38%)
		Ig4_Robo	368..457	CDD:143203	32/88 (36%)
		I-set	458..545	CDD:254352	33/86 (38%)
		Ig	462..536	CDD:299845	27/73 (37%)
		FN3	564..650	CDD:238020	33/88 (38%)
		FN3	687..773	CDD:238020	24/91 (26%)
		fn3	781..867	CDD:278470	30/86 (35%)
robo1	NP_476899.1	Ig	56..151	CDD:299845	55/97 (57%)
		I-set	56..150	CDD:254352	55/96 (57%)
		I-set	157..251	CDD:254352	44/93 (47%)
		Ig2_Robo	159..251	CDD:143201	42/91 (46%)
		I-set	255..341	CDD:254352	34/85 (40%)
		Ig3_Robo	272..341	CDD:143202	30/68 (44%)
		IG_like	351..436	CDD:214653	33/87 (38%)
		Ig	362..444	CDD:299845	32/85 (38%)
		I-set	445..531	CDD:254352	33/86 (38%)
		IGc2	459..521	CDD:197706	22/62 (35%)
		FN3	549..637	CDD:238020	32/87 (37%)
		FN3	673..762	CDD:238020	24/94 (26%)
		fn3	770..855	CDD:278470	30/86 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6690
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	609	1.000	Inparanoid score	I923
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8568
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3358
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	10	9.990