Protein Alignment RAF1 and PKC1

DIOPT Version :10

• Sequence 1: NP_001341618.1
• Gene: RAF1 / 5894
• HGNCID: 9829
• Length: 668
• Species: Homo sapiens
• Sequence 2: NP_009445.2
• Gene: PKC1 / 852169
• SGDID: S000000201
• Length: 1151
• Species: Saccharomyces cerevisiae
• Alignment Length: 850
• Identity: 160/850 - (18%)
• Similarity: 280/850 - (32%)
• Gaps: 284/850 - (33%)

Human     5 QGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASD-DGKLTDPSKTS-NTIRVFLPNKQR 67
            |..|...||..|......:..|.::.|..........|.: .|:.|..|:.| |:..|..|:.|.
Yeast   311 QQGWVNASNINGGSSLASEEGSTLTSTYSNSAIQSTSAKNVQGENTSTSQISTNSWFVLEPSGQI 375

Human    68 TVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLD 132
            .:.     :..|         :..|.|...:...||.|             .::|..:.:: |..
Yeast   376 LLT-----LGFH---------KSSQIERKQLMGGLHRH-------------GAIINRKEEI-FEQ 412

Human   133 HVPLTTHNFARKTFLKLAFCDICQKFL-LNGFRCQTCGYKFHEHCSTKVPTMCV---------DW 187
            |    .|:|.:|:|..:..|..|..|| ..||:||.|.:..|:.|.|.|.|.|:         |.
Yeast   413 H----GHHFVQKSFYNIMCCAYCGDFLRYTGFQCQDCKFLCHKKCYTNVVTKCIAKTSTDTDPDE 473

Human   188 SNIRQLL---LFPNSTIG----------------------DSG----------VPALPSLTM--- 214
            :.:...:   ..|.|..|                      :.|          ||....::|
Yeast   474 AKLNHRIPHRFLPTSNRGTKWCCHCGYILPWGRHKVRKCSECGIMCHAQCAHLVPDFCGMSMEMA 538

Human   215 ----------RRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQR---------QRSTST 260
                      :|.:|...|...|:|...|...|    ..|..|...|::|         ::...|
Yeast   539 NKILKTIQDTKRNQEKKKRTVPSAQLGSSIGTA----NGSDLSPSKLAERANAPLPPQPRKHDKT 599

Human   261 PNVHMVSTTLP-------VDSRMIENNNLSASPRAWSRRFCLRGRDAIRSHSESASPSALSSSP- 317
            |:...|....|       :|.|:    :|....|....:| :...:|..:.:|.|..:|..|||
Yeast   600 PSPQKVGRDSPTKQHDPIIDKRI----SLQTHGREKLNKF-IDENEAYLNFTEGAQQTAEFSSPE 659

Human   318 NNLSPTG-----------------WSQPKTPV-------PAQRERAPVSGTQEKNKIRPRGQRD- 357
            ..|.||.                 |......:       .||||...:...|:..:|:...:.|
Yeast   660 KTLDPTSNRRSLGLTDLSIEHSQTWESKDDLMRDELELWKAQREEMELEIKQDSGEIQEDLEVDH 724

Human   358 -----------------------------------SSYYWEIE----ASEVMLST---------- 373
                                               :|..::||    :.||:..|
Yeast   725 IDLETKQKLDWENKNDFREADLTIDSTHTNPFRDMNSETFQIEQDHASKEVLQETVSLAPTSTHA 789

Human   374 ---------------------------------------RIGSGSFGTVYKGKWHGD---VAVKI 396
                                                   .:|.|:||.|...|....   .|:|:
Yeast   790 SRTTDQQSPQKSQTSTSAKHKKRAAKRRKVSLDNFVLLKVLGKGNFGKVILSKSKNTDRLCAIKV 854

Human   397 LK----VVDPTPEQFQAFRNEVAVLRKTRHVNIL-LFMGYMTKDNLAIVTQWCEGSSLYKH---- 452
            ||    :.:...|..:|.:....:..||:|..:. |:..:.|::.:....::..|..|..|
Yeast   855 LKKDNIIQNHDIESARAEKKVFLLATKTKHPFLTNLYCSFQTENRIYFAMEFIGGGDLMWHVQNQ 919

Human   453 -LHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRW 516
             |.|:..||       .|.:....:.|.|...:|:||:|..||.|.....:||.|:||.. ...|
Yeast   920 RLSVRRAKF-------YAAEVLLALKYFHDNGVIYRDLKLENILLTPEGHIKIADYGLCK-DEMW 976

Human   517 SGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQI---- 577
            .|: :.....|:..:||||:::.|:   ::...|.:::|::||:::..:.|:|. ::.|::
Yeast   977 YGN-RTSTFCGTPEFMAPEILKEQE---YTKAVDWWAFGVLLYQMLLCQSPFSG-DDEDEVFNAI 1036

Human   578 --------------IFMVGRGYASPDLSKLYKNCPK---------AMKRLVADCVKKVKEERPLF 619
                          |..:.:|..:.|..|.....|:         ..:.:..|.:..::.:.|..
Yeast  1037 LTDEPLYPIDMAGEIVQIFQGLLTKDPEKRLGAGPRDADEVMEEPFFRNINFDDILNLRVKPPYI 1101

Human   620 PQILS 624
            |:|.|
Yeast  1102 PEIKS 1106

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RAF1	NP_001341618.1	RBD_CRAF	55..131	CDD:340655	12/76 (16%)
		C1_A_C-Raf	136..187	CDD:410420	19/60 (32%)
		STKc_C-Raf	356..638	CDD:271051	70/397 (18%)
PKC1	NP_009445.2	HR1_PKC-like_1_fungi	1..72	CDD:212011
		HR1_PKC-like_2_fungi	110..181	CDD:212010
		C2_fungal_Pkc1p	211..319	CDD:176071	2/7 (29%)
		C1_ScPKC1-like_rpt1	413..464	CDD:410372	20/54 (37%)
		C1_ScPKC1-like_rpt2	478..536	CDD:410373	6/57 (11%)
		STKc_PKC	828..1145	CDD:270722	63/291 (22%)