Protein Alignment RAF1 and raf1b

DIOPT Version :9

• Sequence 1: NP_001341618.1
• Gene: RAF1 / 5894
• HGNCID: 9829
• Length: 668
• Species: Homo sapiens
• Sequence 2: NP_001186684.1
• Gene: raf1b / 557109
• ZFINID: ZDB-GENE-090826-2
• Length: 629
• Species: Danio rerio
• Alignment Length: 660
• Identity: 501/660 - (75%)
• Similarity: 553/660 - (83%)
• Gaps: 39/660 - (5%)

Human     1 MEHIQGAWKTI-SNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPN 64
            |||.|||||:: |:|||.||:||: .||:||:|::.|.| ||.||||::.| ||||:||||||||
Zfish     1 MEHFQGAWKSVSSHGFGCKDSVFE-DSCMSPSILKTFPY-RRLSDDGRIPD-SKTSSTIRVFLPN 62

Human    65 KQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVD 129
            |||||||||.||:|.:||:||||||||||||||||:|..||..||.|:||:||:.||:||||.|:
Zfish    63 KQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLVGEELLVE 127

Human   130 FLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLL 194
            .|||||||||||.||||||||||||||||||:|||||||||||||||||||||||||||||||||
Zfish   128 VLDHVPLTTHNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLL 192

Human   195 LFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTS 259
            |||  |.|::|...||.|:.|||:.  ||...|..||||||:|..:.|..||...||..:..|||
Zfish   193 LFP--TPGENGALTLPPLSSRRMQN--SRFSFSPYHRYSTPNALNYITPLPSPGVSLGHKVLSTS 253

Human   260 TPNVHMVSTTLPVDSRMIENNNLSASPRAWSRRFCLRGRDAIRSHSESASPSALSSSPNNLSPTG 324
            ||||||||||.|||....:                    :|:| |.|       |.||.|||||.
Zfish   254 TPNVHMVSTTQPVDEAAYQ--------------------EALR-HDE-------SGSPQNLSPTE 290

Human   325 WSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWH 389
            ||  |||.||.||:||.|.|||| :||||.:|||||||||||:||:|.:||||||||||||||||
Zfish   291 WS--KTPAPAHREKAPSSNTQEK-RIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYKGKWH 352

Human   390 GDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLH 454
            ||||||||||::|||||.||||||||||||||||||:||||||||..||||||||||||||||||
Zfish   353 GDVAVKILKVINPTPEQLQAFRNEVAVLRKTRHVNIVLFMGYMTKVKLAIVTQWCEGSSLYKHLH 417

Human   455 VQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGS 519
            ||||.||||||:|||||||||||||||||||||||||||||||||||||||||||||||:|||||
Zfish   418 VQETNFQMFQLMDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKARWSGS 482

Human   520 QQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRG 584
            .:||||:||:|||||||||||||||:||||||||||||||||||||||||.|.||||||||||||
Zfish   483 HRVEQPSGSILWMAPEVIRMQDNNPYSFQSDVYSYGIVLYELMTGELPYSMIGNRDQIIFMVGRG 547

Human   585 YASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAA 649
            |.|||||||||:|||||||||:||::|.||||||||||||||||||||||||||||||||||||.
Zfish   548 YLSPDLSKLYKSCPKAMKRLVSDCIQKTKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAT 612

Human   650 HTEDINACTL 659
            ||||:|.|||
Zfish   613 HTEDMNVCTL 622

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RAF1	NP_001341618.1	Raf_RBD	57..131	CDD:176411	55/73 (75%)
		C1_1	139..187	CDD:278556	45/47 (96%)
		STKc_C-Raf	356..638	CDD:271051	255/281 (91%)
raf1b	NP_001186684.1	Raf_RBD	55..129	CDD:176411	55/73 (75%)
		C1_1	137..185	CDD:278556	45/47 (96%)
		PKc_like	319..601	CDD:328722	255/281 (91%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C194336114
Domainoid	1	1.000	165	1.000	Domainoid score	I16092
eggNOG	1	0.900	-	-		E2759_KOG0193
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H48145
Inparanoid	1	1.050	995	1.000	Inparanoid score	I2347
NCBI	1	1.000	-	-
OMA	1	1.010	-	-		QHG49873
OrthoDB	1	1.010	-	-		D106150at7742
OrthoFinder	1	1.000	-	-		FOG0001587
OrthoInspector	1	1.000	-	-		oto50283
orthoMCL	1	0.900	-	-		OOG6_100006
Panther	1	1.100	-	-	O	PTHR44329
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X994
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	1	1.500	-	-
	16	16.270