Protein Alignment RAF1 and mph1

DIOPT Version :10

• Sequence 1: NP_001341618.1
• Gene: RAF1 / 5894
• HGNCID: 9829
• Length: 668
• Species: Homo sapiens
• Sequence 2: NP_595150.2
• Gene: mph1 / 2540060
• PomBaseID: SPBC106.01
• Length: 678
• Species: Schizosaccharomyces pombe
• Alignment Length: 562
• Identity: 120/562 - (21%)
• Similarity: 219/562 - (38%)
• Gaps: 109/562 - (19%)

Human   174 EHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALP--------SLTMRRMRESVSRM----PV 226
            ||..|.:|:.....|.: .|....|....:|....||        ||::.| |..:.|:    |.
pombe   108 EHLITPLPSTNASHSEV-SLSAGVNDLNSNSEHDLLPKSVNKTPGSLSISR-RRRIGRIGLGPPK 170

Human   227 SSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTL-PVDSRMIENNNLSAS----- 285
            .:::..:.|...:...:|..::..:..:.|..|..:..:.:||| |:   .:.|..|..|
pombe   171 RAEYTLTDPSKTSDTKNSTEADEDIEMKSREVSPASNSVAATTLKPL---QLHNTPLQTSQEHPK 232

Human   286 ----PRAWSRRFCLR---GRDAIRSHSESAS-PSALSSSPNNLSPTGWSQPKTPVPAQRERAPVS 342
                |..:...|..|   ..|..|..||..| |..:...|...:    |||........:.||:.
pombe   233 PSFHPSQFESSFSPRVQFDHDVERRASELHSRPVTVFQEPQRSA----SQPYESHALSPKVAPLF 293

Human   343 GTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGD----VAVKILKVVDPT 403
            ...:...| |:.|:|......::..::.:   :|.|....||: .:..|    .|:|.:..::..
pombe   294 DNSQATPI-PKRQQDVVTVANLQFIKLGV---VGKGGSSMVYR-IFSPDNSRLYALKEVNFINAD 353

Human   404 PEQFQAFRNEVAVLRK-TRHVNILLFMGYMTKDNLAIVTQWCE-GSSLYKHLHVQETK------- 459
            ....|.::||:|:||| :.:..|:........|.|..:....| |.:...:|.::..|
pombe   354 QTTIQGYKNEIALLRKLSGNDRIIKLYAAEVNDTLGQLNMVMECGETDLANLLMKNMKKPINLNF 418

Human   460 FQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQ 524
            .:|:.     .|..:.:..:|.:||:|.|:|..|..|.|| .:|:.|||:|......:.:...:.
pombe   419 IRMYW-----EQMLEAVQVVHDQNIVHSDLKPANFLLVEG-NLKLIDFGIAKAIGNDTTNIHRDS 477

Human   525 PTGSVLWMAPEVIRMQDNNPFSFQ----------SDVYSYGIVLYELMTGELPYSHINNRDQIIF 579
            ..|::.:||||.  :.|.|..:..          |||:|.|.:||:::.|..|::|:.....|..
pombe   478 HIGTINYMAPEA--LTDMNAHTNSGVKLVKLGRPSDVWSLGCILYQMVYGRAPFAHLKMIQAIAA 540

Human   580 MVGRGY-------------------------ASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLF 619
            :....|                         ..|||..:       |||    |:::.:.:|...
pombe   541 IPNEQYHIHFPEVALPANAVQEKEGSLPGVTVGPDLMDV-------MKR----CLERDQRKRLTI 594

Human   620 PQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTT 661
            |::|  :....:.||......::..|..:.||.:.:...|:|
pombe   595 PELL--VHPFLNPLPSYLTPLAKKPLPVSGHTNNAHPLRLST 634

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RAF1	NP_001341618.1	RBD_CRAF	55..131	CDD:340655
		C1_A_C-Raf	136..187	CDD:410420	4/12 (33%)
		STKc_C-Raf	356..638	CDD:271051	70/329 (21%)
mph1	NP_595150.2	PKc_Mps1	315..603	CDD:271033	67/312 (21%)