Protein Alignment ALS2 and HERC2

DIOPT Version :10

• Sequence 1: NP_065970.2
• Gene: ALS2 / 57679
• HGNCID: 443
• Length: 1657
• Species: Homo sapiens
• Sequence 2: NP_608388.2
• Gene: HERC2 / 33035
• FlyBaseID: FBgn0031107
• Length: 4912
• Species: Drosophila melanogaster
• Alignment Length: 1682
• Identity: 324/1682 - (19%)
• Similarity: 491/1682 - (29%)
• Gaps: 730/1682 - (43%)

Human     7 SSTEAEGSKERGL---VHIWQAGSFPITPERLPGWGGKTV--------------LQAALGVKHGV 54
            |..||..:.|:.|   |.:|....    .|:|.|..|..|              :..|.|.|...
  Fly  2972 SHEEAAAAPEQDLPCTVMVWGLND----KEQLGGLKGSKVKVPTFSQTISRLRPIHIAGGSKSLF 3032

Human    55 LLTEDGEVYSFGT-LPWRSG-----PVEICPSSPILENALVGQYV---ITVATGSFHSGAVTDNG 110
            ::::||:||:.|. ...|.|     .|.:....|:|.     |||   :.|.:|..|:.|:|.:|
  Fly  3033 IVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLR-----QYVVKKVAVHSGGKHALALTLDG 3092

Human   111 VAYMWGENSAGQCAVANQQYVPEPNPVSIADSEASPLLAVRILQLACGEEHTLALSISREIWAWG 175
            ..:.|||...|:....|:..:.:|..|     ||  |.|.:|..:|||..|:.|:|...|::.||
  Fly  3093 KVFSWGEGEDGKLGHGNRTTLDKPRLV-----EA--LRAKKIRDVACGSSHSAAISSQGELYTWG 3150

Human   176 TGCQLGLI-----TTAFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQC 235
            .| :.|.:     ||..   ||:.|..||||.|:|||||            |:|.:
  Fly  3151 LG-EYGRLGHGDNTTQL---KPKLVTALAGRRVVQVACG------------SRDAQ--------- 3190

Human   236 SQLLITMTDKEDHVIIS--DSHCCPLGVTLTESQAENHASTALSPSTETLDRQEEVFENTLVAND 298
                 |:...||..:.|  |.....||
  Fly  3191 -----TLALTEDGAVFSWGDGDFGKLG-------------------------------------- 3212

Human   299 QSVATELNAVSAQITSSDAMSSQQNVMGTTEISSARNIPSYPDTQAVNEYLRKLSDHSVREDSEH 363
                                                                       |..||.
  Fly  3213 -----------------------------------------------------------RGGSEG 3218

Human   364 GEKPVPSQPLLEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTT 428
            .:.|...:.|                           |.:||                      .
  Fly  3219 SDTPHEIERL---------------------------SGIGV----------------------V 3234

Human   429 PCETGAQAGSSAIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEETEGGSRRLSLPGLLSQVSP 493
            ..|.|||..
  Fly  3235 QIECGAQFS-------------------------------------------------------- 3243

Human   494 RLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDG 558
                           |..|.:|           ||||||||...:||||......:|..:..|.|
  Fly  3244 ---------------LALTRAG-----------EVWTWGKGDYYRLGHGGDQHVRKPQPIGGLRG 3282

Human   559 KEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSD-FPTTVP------------RLAKISSEN 610
            :.|||:..|..|.||:|...|||:||.|..||.|..: |....|            |:|..||.:
  Fly  3283 RRVIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGSGNTFVNKKPALVIGLDAVFVNRVACGSSHS 3347

Human   611 GVWSIA-AGRDYSLFLVDTEDFQPGLYYSGRQDPTEGDNL----------------PENHS---- 654
            ..|.:. |..|        |:.:..:.:|..:||..|.:|                |.|.|
  Fly  3348 IAWGLPNASSD--------EEKRGPVPFSSTRDPLGGSSLGIYEAETMQTLKQEAKPLNQSSLSE 3404

Human   655 --GSKTP-------------------------VLLSCSKLGYISR-VTAGKDSYLALVDKNIMGY 691
              ..:||                         .|.|.||:.:..| ...|::.:  |:...|||.
  Fly  3405 SLALETPAARQAALGHVLRAMSILQARQLIVAALTSHSKVNFKERGAVGGEEDH--LIGGPIMGA 3467

Human   692 IASLHE-LATTERRFYSKLSDIKSQILRPLLSLENL------GTTTTVQLLQEVASRFSKLCYLI 749
            ...|.| :|.......:..:|...|...|..:::.|      |..|...|.....|.|..|...:
  Fly  3468 PLQLAETIAQGGGEAPADATDAGLQEHSPEAAVDALTGGMSGGANTLPPLSAGPLSAFQSLTGSL 3532

Human   750 GQHGASLSSFL----HGVKEARSLVILKHSSLFLD---SYTEYCTSITNFLVMGGFQLLAKPAID 807
            ...|:..||.|    |....|.::.::..:....:   |:..:|..:.:|   ||  ||.:|
  Fly  3533 SMSGSLSSSALPQHKHSRMSASAMSVMAATMTQQEEMLSHISHCHGLDDF---GG--LLGEP--- 3589

Human   808 FLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLATCFEVASPE-YQKLQDS 871
                             |...|:|                   |||||.|.....|. .|.:.|:
  Fly  3590 -----------------EAKSLVE-------------------LLKLAVCGRCGPPSTSQTIADT 3618

Human   872 ----------------SSCYE-----CLALH-LGRKRK----EAEY-------TLGFWKTFPGKM 903
                            .:|..     |.:.| ||:..|    |:.:       ..|..: .||..
  Fly  3619 LISLGAGTPAVAAMLLETCITELEDLCTSRHCLGKLPKPVMQESSHPYVDNVNVTGVVR-IPGAE 3682

Human   904 T-----DSLRKPERR----LLCESSNRALSLQHAGRFSVNW------------FILFNDALVHA- 946
            .     ||....|:|    ::.:.:.|.|::: :||...:|            :...:|:.|:.
  Fly  3683 MLRLEFDSQCSTEKRNDPLVIMDGTGRVLAMR-SGREFAHWAPEIRVLGDELRWKFSSDSSVNGW 3746

Human   947 --QFSTHHVFPLATLWAEPLSEEAGGVNGLKITTPEEQFTLISS------TPQEKTKWLRAISQA 1003
              :|..|.:.|.||| .|..|:.|        ...:....|:.|      .|::.:..||..|
  Fly  3747 GWRFWVHAIMPAATL-GESGSDRA--------VLSQPSMALVMSLLDSRLAPRQPSVLLRLAS-- 3800

Human  1004 VDQALRGMSDLPPYGSGSSVQR-------------------QEPPIS--------RSAKYTFYKD 1041
               ||...|.|   |:.::.||                   |:|.:|        ...:...|::
  Fly  3801 ---ALAACSQL---GALTTAQRIWSLRKLHAVLLLEQAPRPQDPSLSTLLQPLIPELLRQYEYEE 3859

Human  1042 PRLK---------------------------DATYDGRWLSGKPHG----RGVLKWPDGKMY--- 1072
            |:::                           .||......|..|..    ..|.||...|.|
  Fly  3860 PQVRGGIHLMHSDYFKTLAALACDMQLDAALPATSSSSSSSAAPGAISSTGDVHKWAWFKRYCIA 3924

Human  1073 -----SGMFRNGLEDGYG-EYR------IPNKAMNKEDHYVGHWKEGKMCGQGVYSYASGEVFEG 1125
                 |.:.|..|...:. |.|      :|:.:.|...:                        .|
  Fly  3925 VRVAQSLIRRTELPRAFCLEVRKKFAEMLPSSSSNSNAN------------------------PG 3965

Human  1126 CFQDNMRHGHGLLRS-GKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGV 1189
            |    ...|..:|.| ..|:||:.|                                :|....|:
  Fly  3966 C----QSPGASMLNSTTSLSSSTVS--------------------------------NVSPPPGI 3994

Human  1190 VVTQFGLYYEGNFHLNKMMGNGVLLSEDDTIYEGEFS-----------DDWTLSGKGTLTMPN-G 1242
            ...|..|:    .|.:::.....||.||.|:::....           |||.||..|..|:.. |
  Fly  3995 TGEQPDLH----CHAHQLESTSTLLHEDHTLFQAPHDAQLLQWLNRRPDDWALSWGGASTIYGWG 4055

Human  1243 DYIEGYFSGEWGSGIKITGT------YFKP--------SLYESDKDRPKVFR----KLGNLAVPA 1289
            ....|...|..||.|| |.|      ..:|        ||:....| .|:|.    ..|.|.|..
  Fly  4056 HNHRGQLGGLEGSRIK-TPTPCEALSLLRPVQLAGGEQSLFAVTPD-GKLFATGYGSGGRLGVGG 4118

Human  1290 DEKW---------KAVFDECWRQLGCEGPG--------QGEVWKAW-DNIAVALTTSRRQHRDSP 1336
            .:.|         :.||   .:::.....|        :|||: || :.....|....|...|.|
  Fly  4119 SDSWAIPTLLGSLQHVF---VKKVAVNSGGKHCLALTTEGEVY-AWGEGEDGKLGHGNRMSYDRP 4179

Human  1337 EILSRSQTQTLESLEFIPQHVGAFSV------------------EKYDDIRKYLIKA 1375
            :::......::..:.....|..|.:.                  :..|.:|..|::|
  Fly  4180 KLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEA 4236

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ALS2	NP_065970.2	ATS1	<39..219	CDD:444065	62/207 (30%)
		RCC1 1. /evidence=ECO:0000305	59..108		17/57 (30%)
		RCC1 2. /evidence=ECO:0000305	109..167		18/57 (32%)
		RCC1 3. /evidence=ECO:0000305	169..218		20/53 (38%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	432..480		3/47 (6%)
		RCC1 4. /evidence=ECO:0000305	525..576		22/50 (44%)
		ATS1	<527..>683	CDD:444065	58/217 (27%)
		RCC1 5. /evidence=ECO:0000305	578..627		18/62 (29%)
		RhoGEF	695..879	CDD:470622	41/219 (19%)
		PH_alsin	905..1010	CDD:241423	28/129 (22%)
		COG4642	<1049..1249	CDD:443680	42/231 (18%)
		MORN 1	1049..1071		5/25 (20%)
		MORN 2	1072..1094		7/36 (19%)
		MORN 3	1100..1122		0/21 (0%)
		MORN 4	1123..1145		6/22 (27%)
		MORN 5	1151..1173		0/21 (0%)
		MORN 6	1175..1197		3/21 (14%)
		MORN 7	1198..1220		5/21 (24%)
		MORN 8	1221..1244		8/34 (24%)
		VPS9	1552..1656	CDD:460489
HERC2	NP_608388.2	ATS1	625..>894	CDD:444065
		Cyt-b5	1292..1364	CDD:459698
		MIB_HERC2	1928..1988	CDD:461991
		UBA_HERC2	2515..2559	CDD:270585
		Cul7	2624..2699	CDD:463286
		APC10-HERC2	2762..2932	CDD:176485
		ATS1	2987..3320	CDD:444065	123/606 (20%)
		RCC1	3302..3350	CDD:395335	15/47 (32%)
		ATS1	4050..4389	CDD:444065	38/193 (20%)
		HECTc	4509..4880	CDD:238033