Protein Alignment HACE1 and HUWE1

DIOPT Version :9

• Sequence 1: NP_065822.2
• Gene: HACE1 / 57531
• HGNCID: 21033
• Length: 909
• Species: Homo sapiens
• Sequence 2: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Alignment Length: 978
• Identity: 265/978 - (27%)
• Similarity: 414/978 - (42%)
• Gaps: 205/978 - (20%)

Human    18 ARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLV 82
            |:..:||     .:...:|:..:...|.:        :..|  |.:::..:..:...||:.|.|.
  Fly  4293 AKRTQLP-----TLSVALPVTPSKPRRKI--------YSKN--FSQLQLVIEVLTHQCGTTEGLD 4342

Human    83 LLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELL 147
            .:.|...|     ::.|:....|.          .::|           |||    |:.|..|.:
  Fly  4343 NVAKLIVN-----LTQCSQASSAI----------FIQY-----------LTA----AILGLAEEV 4377

Human   148 HDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINR----PNVSGATPLYFACSH- 207
            ...:|.:        :.:..::...|.|..||.|....|||....    |.||||:.|.....|
  Fly  4378 RQAIQML--------LNEIRMYNIHQGGAGTTSQAASTSGAAAATASYVPTVSGASLLPNLAVHE 4434

Human   208 ---GQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLR 269
               ..|.||:.:::...|      ...|           |||:.:       .::.::..|...:
  Fly  4435 GIMQDRFTAEHVIISAPK------NAKP-----------TCELQL-------PSMKKLMSNSSAQ 4475

Human   270 ENMLR--QVLEHLSQQSESQY-LKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVF 331
            ...||  ::...:....|:|. |.....:|..|..|...::.:..:..|...|...:.......
  Fly  4476 PYFLRTLKIFMQVRDMYEAQNGLSTGGGVACGAGDNDDDIIDIEGDGGASGGSSSDMAPGSAST- 4539

Human   332 RIGPSSPSNGIDMGYNGNK-------------------TPRSQVFKP-------LELLWHSLDEW 370
             |.|..|.....:..:|:.                   ...|:..||       |::||::|.|.
  Fly  4540 -ISPPPPMETNQIAQDGDDDEEDDDEARSFPGVGNHAVASASKSTKPTLSQILCLDVLWNTLSEC 4603

Human   371 LVLIATELMKNKRDSTEITSILL------------------KQKGQD--QDAASIPPFEPPGPGS 415
            ||.:       :....|...::|                  |:.|:.  .:||........|.|:
  Fly  4604 LVAL-------EESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGA 4661

Human   416 YENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSP------ 474
            ...|..    :.|.|    ||.|....||.|......::......|..:....|.:|:|
  Fly  4662 IGGLGA----AAPSA----QEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAM 4718

Human   475 ------------RFIEFVCKHDEVLKCFVNRNP--------KIIFDHFHFLLECPELMSRFMHI- 518
                        :|::|..||..||...:.::|        .::.||           :|.:..
  Fly  4719 SAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDH-----------TRILDFD 4772

Human   519 IKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIA 583
            :|.:.|:...|...|.:.             .|...:.|.|.::|..|..|:.:....:.|....
  Fly  4773 VKRKYFQTELERLDEGIR-------------REEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFY 4824

Human   584 VRFHGEEGM-GQGVVREWFDILSNEIVNPDYALFTQS-ADGTTFQPNSNSYVNPDHLNYFRFAGQ 646
            :.|..|||. ..|::|||:.|:|.||.||.||||..| .|..|:..|.:|:.||:||:||:|.|:
  Fly  4825 IVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGR 4889

Human   647 ILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVE 711
            ::..|::..:|:..||||||||||||..|.:.|:.|.|.|:.|.|.:::.||||.||.|||||.|
  Fly  4890 VIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTE 4954

Human   712 TDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLF 776
            ...||..:...|||.|....||:.||.||||||.:|:|:.:|:.|::|||:||:..||..||.:|
  Fly  4955 VQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIF 5019

Human   777 DEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRV 841
            :|.|||||:||:|:||:.|...||||.. |..:...|||||..:....|.:|...||||||:|:|
  Fly  5020 NEQELELLISGLPDIDIEDLKANTEYHK-YTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKV 5083

Human   842 PHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGY 906
            |..||.::.|.:|:|.|.|.....:.:.||.:.||.|.|.||.|.|.:.|:..||.|:|..|.|:
  Fly  5084 PLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGF 5148

Human   907 TMA 909
            ..|
  Fly  5149 GFA 5151

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HACE1	NP_065822.2	Ank_2	<27..>283	CDD:330894	46/265 (17%)
		ANK 1	64..93		6/28 (21%)
		ANK repeat	66..95	CDD:293786	6/28 (21%)
		ANK	92..217	CDD:238125	28/132 (21%)
		ANK repeat	97..128	CDD:293786	3/30 (10%)
		ANK 2	97..126		3/28 (11%)
		ANK repeat	130..161	CDD:293786	7/30 (23%)
		ANK 3	130..159		7/28 (25%)
		ANK repeat	163..194	CDD:293786	8/34 (24%)
		ANK 4	163..192		8/28 (29%)
		ANK 5	196..226		9/33 (27%)
		ANK repeat	196..221	CDD:293786	8/28 (29%)
		ANK 6	228..257		4/28 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	398..433		7/36 (19%)
		HECTc	554..903	CDD:238033	155/350 (44%)
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740
		DUF4414	3675..3779	CDD:291075
		HECTc	4796..5149	CDD:238033	157/353 (44%)
		HECTc	4820..5148	CDD:214523	151/328 (46%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D140374at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR11254
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	4	3.920