Protein Alignment Nox and Duox1

DIOPT Version :10

• Sequence 1: NP_611116.2
• Gene: Nox / 5740310
• FlyBaseID: FBgn0085428
• Length: 1340
• Species: Drosophila melanogaster
• Sequence 2: NP_001092767.1
• Gene: Duox1 / 99439
• MGIID: 2139422
• Length: 1551
• Species: Mus musculus
• Alignment Length: 1329
• Identity: 260/1329 - (19%)
• Similarity: 441/1329 - (33%)
• Gaps: 549/1329 - (41%)

  Fly   156 QRQSNLYMERYNLNLDK---NCSSMELEARREKQRWLLI-------------------------S 192
            |||     :|.::..:|   ...::|.:.|.|..|.:|:                         .
Mouse   628 QRQ-----DRQSIMSEKLVGGVEALEWQGRNEPCRPVLVHLQPGQIRVVDGRLTVLRTIQLRPPQ 687

  Fly   193 ECSALFDEGEGKHTREAFRKLFLDEEFQQKLFQLFDLERNGYLLQDRWIEHLKGRLTDD----RQ 253
            :.:.:.....|:.|      |.|....:..|..||::|.....|    :|:::|.|.::    ::
Mouse   688 QVNLILSSNRGRRT------LLLKIPKEYDLVLLFNMEEERQAL----VENVRGALKENGLSFQE 742

  Fly   254 MDFAEQIESVAYVICGENKRVSFKNFRDIWHTRG-ILDKLYRLIELDGSNLVSTNQVMEFISHLT 317
            .:..||          |..|.:...     ..|| :|:..:|       :|.|  ||::......
Mouse   743 WELREQ----------ELMRAAVTR-----QQRGHLLETFFR-------HLFS--QVLDINQADA 783

  Fly   318 NSRPRTGFDKSSLARLEQLFRTTVGNEQEIRREEFQKIVTSK-NPFFTERVFQIFDKDNSGSISL 381
            .:.|   .|.|:..| |.|       ..|:.|.||...:..| ...|.|.:|.:.|||.:|.:|.
Mouse   784 GTLP---LDSSTKVR-EAL-------TCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYLSF 837

  Fly   382 QEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRHCIKENGMEFSEDQIEDLTSA 446
            :||:|.:..|...|.::|.|.:|::||.||:|||...|...::|..|:.:....|:.|:.::..:
Mouse   838 REFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVES 902

  Fly   447 MFEDADPHNSGEITYEALKNQLHKHGG----------------LLENL----------------- 478
            ||.::...:..|:|:|.....|..|..                :::||
Mouse   903 MFRESGFQDKEELTWEDFHFMLRDHDSDLRFTQLCVKGVEVPEVIKNLCRRASYISQEKICPSPR 967

  Fly   479 ------------SITIDRWLVPIAED------RQAG------------GAAKSGFWNSLPH---- 509
                        :.:..|...|:..|      |:.|            .|.:..|..|..|
Mouse   968 MSAHCARNNMKTASSPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSRRHQTVQ 1032

  Fly   510 QFSLAYMKNNQVFVTYLFFYITVNLCLFISRAIQYRAS---NGF-------VIIARACGQCLNFN 564
            ||. .:::|.:..:..:..:.|:...||:.||..|..:   :|.       :|::|.....::|.
Mouse  1033 QFK-RFIENYRRHIGCVAVFYTITGALFLERAYYYAFAAHHSGITDTTRVGIILSRGTAASISFM 1096

  Fly   565 CAWVLVLMLRHSLTYLRGRGLSSYLPLDHHVYLHKLTGITISVLSLIHTIMHLFNFSIIVINDPN 629
            .:::|:.|.|:.:|:||...|:.|:|.|..|..|:|...|..:|:::|:..|:.|..:..|:..:
Mouse  1097 FSYILLTMCRNLITFLRETFLNRYIPFDAAVDFHRLIASTAIILTVLHSAGHVVNVYLFSISPLS 1161

  Fly   630 INAGHYTIGEWLLTDRPGL----------------FGLIPGCANPTGVALLAILVVMFVCSQPFV 678
            :.:..:          |||                |..:||.   |||.||..|.:|:|.:....
Mouse  1162 VLSCLF----------PGLFHDDGSEFPQKYYWWFFQTVPGL---TGVLLLLALAIMYVFASHHF 1213

  Fly   679 RRKGSFEVFYWTHLLYVPFWILCLFHG-------PNFWKWFLLPGLVYIVERALRFIWMRGEHGK 736
            ||: ||..|:.||.||:..:||.:.||       |.|..:||:|.::|:.::.:..     ...|
Mouse  1214 RRR-SFRGFWLTHHLYIFLYILLIIHGSFALIQMPRFHIFFLVPAIIYVGDKLVSL-----SRKK 1272

  Fly   737 TYIS--SGLLLPSKVVHLVIKRPHHFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHI 799
            ..||  ...||||.|.||..:||..|.::.|.:|.:...|:...|:||||::||| .||.:.|||
Mouse  1273 VEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAP-HEDTLSLHI 1336

  Fly   800 RTVGEWTNRLYRYFEREQQKLQQSGSSQEIPQHMHAIPTPSFMLLNEARNPAIAGERSATPQTDF 864
            |..|.||.||
Mouse  1337 RAAGPWTTRL------------------------------------------------------- 1346

  Fly   865 LAKNLGVQAVPPVRPPRQNRKPAPGAPIDPPATGVNRIRSIKKTLQRTFSRKEAVDPKKGIPNGA 929
                                                               :|...|
Mouse  1347 ---------------------------------------------------REIYSP-------- 1352

  Fly   930 FIADGEREDSNLKQRPLEKSISLPDISVKSKKRSRLKALRALGRSESESAFDEKRVRRARNNSVG 994
                                                                             
Mouse  1353 ----------------------------------------------------------------- 1352

  Fly   995 LAYLSPQNKSLAQSFRYMRTKPTIIAFKTPSMEEREHQVAAGEANGASPASRAEQGQLSSRMDSA 1059
                                                                             
Mouse  1353 ----------------------------------------------------------------- 1352

  Fly  1060 DKLQLARLSLSAEGASKPLEDQTQTGSPSRKSILRRPTFLRSLSASINNRTGGGGGGSTGSSTTN 1124
                                       |:..:..|.|
Mouse  1353 ---------------------------PTGDTCARYP---------------------------- 1362

  Fly  1125 SGGKVTLDAGVMEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHSCPR 1189
                        ::::|||:|.........:.:||:|.|||||||||||:.::.:      |...
Mouse  1363 ------------KLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFK------SSVS 1409

  Fly  1190 CQFEWASEIPKSVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGAMERFLDMHMYITSA 1254
            ||           :..:|:.|.|:.|.||.|||..:::.  |:|:.:    .:..:.:|:|||..
Mouse  1410 CQ-----------VFCKKIYFIWVTRTQRQFEWLADIIR--EVEEND----RQDLVSVHIYITQL 1457

  Fly  1255 LQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQA--QQKGKVTVFYCGP 1317
            .::.|::...|.:......:...|.|.|||::.|:.|||.::..|..||.  .|..|:.||.|||
Mouse  1458 AEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGP 1522

  Fly  1318 PQLAKTLRYKC------DQYGFAFRKECF 1340
            |.:.|.:...|      |:..|:...|.|
Mouse  1523 PGMTKNVEKACQLINRQDRTHFSHHYENF 1551

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nox	NP_611116.2	EFh_HEF	222..425	CDD:355006	55/208 (26%)
		EF-hand motif	290..316	CDD:320075	5/25 (20%)
		FRQ1	331..462	CDD:444056	40/131 (31%)
		EF-hand motif	351..388	CDD:320075	14/37 (38%)
		EFh	365..425	CDD:238008	23/59 (39%)
		EF-hand motif	402..425	CDD:320075	9/22 (41%)
		PLN02844	<544..>816	CDD:215453	92/306 (30%)
		FNR_like	<1136..>1178	CDD:447143	18/41 (44%)
		NAD_binding_6	1158..1325	CDD:429792	55/168 (33%)
Duox1	NP_001092767.1	dual_peroxidase_like	32..591	CDD:188652
		EFh	820..881	CDD:238008	23/60 (38%)
		EFh	857..915	CDD:238008	16/57 (28%)
		PLN02292	1089..>1412	CDD:215165	114/670 (17%)
		NOX_Duox_like_FAD_NADP	1277..1551	CDD:99783	100/608 (16%)