Protein Alignment Nox and Cybb

DIOPT Version :10

• Sequence 1: NP_611116.2
• Gene: Nox / 5740310
• FlyBaseID: FBgn0085428
• Length: 1340
• Species: Drosophila melanogaster
• Sequence 2: NP_076455.2
• Gene: Cybb / 66021
• RGDID: 620574
• Length: 570
• Species: Rattus norvegicus
• Alignment Length: 905
• Identity: 189/905 - (20%)
• Similarity: 286/905 - (31%)
• Gaps: 423/905 - (46%)

  Fly   518 NNQVFVTYLFFYITVNLCLFISRAIQY----------RASNGFVIIARACGQCLNFNCAWVLVLM 572
            |..:.:..:..::.:|:.||:.....|          :.....:.:|||...||||||..:|:.:
  Rat     7 NEGLSIFVILVWLGLNVFLFVKYYKVYDDEPKYNYTRKLLGSALALARAPAACLNFNCMLILLPV 71

  Fly   573 LRHSLTYLRGRG--LSSYL--PLDHHVYLHKLTGITISVLSLIHTIMHLFNFSIIV-----INDP 628
            .|:.|::|||..  .|:.:  .||.::..||:....|::.:.||||.||||....|     .:||
  Rat    72 CRNLLSFLRGSSACCSTRIRRQLDRNLTFHKMVAWMIALHTAIHTIAHLFNVEWCVNARVGTSDP 136

  Fly   629 N----INAGHYTIGEWLLTDRP-------GLFGLIPGCANPTGVALLAILVVMFVCSQPFVRRKG 682
            .    .|.|.....|:|...|.       ||:..:...|..||:.:...|:::...|...:|| .
  Rat   137 YSVALSNIGDKENEEYLNFAREKIKNPEGGLYVAVTRLAGITGIVITLCLILIITSSTKTIRR-S 200

  Fly   683 SFEVFYWTHLLYVPFWILCLFHG------------------------------------PNF--- 708
            .||||::||.|:|.|:|....||                                    |.|
  Rat   201 YFEVFWYTHHLFVIFFIGLAIHGAERIVRGQTSDSLKEHNLDVCADKIKEWGKIKECPVPKFAGN 265

  Fly   709 ----WKWFLLPGLVYIVERALRFIWMRGEHGKTYISSGLLLPSKVVHLVIKRPHHFNFRPGDYVF 769
                |||.:.|..:|:.||.:|| |...:  |..|:..:..|.|.:.|.:|: ..|....|.|:|
  Rat   266 PPMTWKWIVGPMFLYLCERLVRF-WRSQQ--KVVITKVVTHPFKTIELQMKK-KGFKMEVGQYIF 326

  Fly   770 VNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYRYFEREQQKLQQSGSSQEIPQHMH 834
            |..|.::..||||||::|||| ||:..:|||.||:||..|:.....::|:.|.
  Rat   327 VKCPQVSKLEWHPFTLTSAPE-EDFFSIHIRIVGDWTEGLFNACGCDKQEFQD------------ 378

  Fly   835 AIPTPSFMLLNEARNPAIAGERSATPQTDFLAKNLGVQAVPPVRPPRQNRKPAPGAPIDPPATGV 899
                                                                             
  Rat   379 ----------------------------------------------------------------- 378

  Fly   900 NRIRSIKKTLQRTFSRKEAVDPKKGIPNGAFIADGEREDSNLKQRPLEKSISLPDISVKSKKRSR 964
                                                                             
  Rat   379 ----------------------------------------------------------------- 378

  Fly   965 LKALRALGRSESESAFDEKRVRRARNNSVGLAYLSPQNKSLAQSFRYMRTKPTIIAFKTPSMEER 1029
                                                                   |:|.|
  Rat   379 -------------------------------------------------------AWKLP----- 383

  Fly  1030 EHQVAAGEANGASPASRAEQGQLSSRMDSADKLQLARLSLSAEGASKPLEDQTQTGSPSRKSILR 1094
                                                                             
  Rat   384 ----------------------------------------------------------------- 383

  Fly  1095 RPTFLRSLSASINNRTGGGGGGSTGSSTTNSGGKVTLDAGVMEIFIDGPYGAPSSHIFGAQHAVL 1159
                                                      :|.:|||:|..|..:|..:..:|
  Rat   384 ------------------------------------------KIAVDGPFGTASEDVFSYEVVML 406

  Fly  1160 IGTGIGVTPFASILQSIMHRYWKARHSCPRCQFEWASEIPKSVMNLRKVDFFWINRDQRSFEWFV 1224
            :|.|||||||||||:|:.::|      |...          :.:.|:|:.|:|:.||..:||||.
  Rat   407 VGAGIGVTPFASILKSVWYKY------CDNA----------TSLRLKKIYFYWLCRDTHAFEWFA 455

  Fly  1225 NLLSQLEIEQAELGGAMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTN 1289
            :||..||.:..|...|  .||..::|:|...:.        |.....:|...::|:|||||.:|.
  Rat   456 DLLQLLETQMQERNNA--NFLSYNIYLTGWDES--------QANHFAVHHDEEKDVITGLKQKTL 510

  Fly  1290 AGRPNWDKVFKQLQAQQKG-KVTVFYCGPPQLAKTLRYKC---DQYG-----FAFRKECF 1340
            .||||||..||.:.:|... ::.||.|||..|||||..:.   .:.|     |.|.||.|
  Rat   511 YGRPNWDNEFKTIASQHPNTRIGVFLCGPEALAKTLSKQSISNSESGPRGVHFIFNKENF 570

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nox	NP_611116.2	EFh_HEF	222..425	CDD:355006
		EF-hand motif	290..316	CDD:320075
		FRQ1	331..462	CDD:444056
		EF-hand motif	351..388	CDD:320075
		EFh	365..425	CDD:238008
		EF-hand motif	402..425	CDD:320075
		PLN02844	<544..>816	CDD:215453	103/344 (30%)
		FNR_like	<1136..>1178	CDD:447143	21/41 (51%)
		NAD_binding_6	1158..1325	CDD:429792	63/167 (38%)
Cybb	NP_076455.2	Ferric_reduct	65..220	CDD:426438	47/155 (30%)
		PLN02844	<165..>423	CDD:215453	90/572 (16%)
		NOX_Duox_like_FAD_NADP	297..570	CDD:99783	112/609 (18%)
		NAD_binding_6	401..550	CDD:429792	64/174 (37%)