Protein Alignment Nox and Duox2

DIOPT Version :10

• Sequence 1: NP_611116.2
• Gene: Nox / 5740310
• FlyBaseID: FBgn0085428
• Length: 1340
• Species: Drosophila melanogaster
• Sequence 2: NP_001349684.1
• Gene: Duox2 / 214593
• MGIID: 3036280
• Length: 1545
• Species: Mus musculus
• Alignment Length: 1243
• Identity: 237/1243 - (19%)
• Similarity: 400/1243 - (32%)
• Gaps: 500/1243 - (40%)

  Fly   235 LLQDRWIEHLKGRLTDDR--QMDFAEQIESVAYVICGENKR-----------VSFKNFRDIWHTR 286
            ||.||.::.|..|.|..|  |:...:|:..:.....|....           :.|.:..|    |
Mouse   666 LLPDRSLQVLDKRFTVLRTIQLQSPQQVNLILSSNSGRRTLLLKIPKEYDLVLMFNSEED----R 726

  Fly   287 GILDKLYR---LIELDGSNLVSTNQVMEFISHLTNSRPRTG----FDKSSLARLEQLFRTTVG-- 342
            |...:|.:   :....|.::...:: .|.:......:.|.|    |.:...|::..:.:...|
Mouse   727 GAFVRLLQDLCICCTPGLHIAEVDE-KELLRKAVTKQQRAGILEIFFRQLFAQVLDINQADAGTL 790

  Fly   343 ---NEQEIR--------REEFQKIVTSK-NPFFTERVFQIFDKDNSGSISLQEFIDAIHQFSGQS 395
               :.|::|        |.||...:..| ...|.|.:|.:.|||.:|.||.:||:|.:..|...|
Mouse   791 PLDSSQQVREALTCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYISFREFLDILVVFMKGS 855

  Fly   396 ADDKIRFLFKVYDIDGDGLIQHKELHDVIRHCIKENGMEFSEDQIEDLTSAMFEDADPHNSGEIT 460
            ::||.|.:|.:||:||:|.:...|...::|..|:.:....|:.|:.::..:||.::...:..|:|
Mouse   856 SEDKSRLMFTMYDLDGNGFLSKDEFFTMMRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELT 920

  Fly   461 YEALKNQLHKHGGLLENLSITIDRWLVPIAEDRQAGGAAKSGFWN------SLPHQFSLA----- 514
            :|.....|..|...|....:.:........:..:...|.:..|.|      |.|.:.:|:
Mouse   921 WEDFHFMLRDHDSDLRFTQLCVKGGAGGTKDIFKQSSACRVSFINRTPGNSSCPPETALSDMETP 985

  Fly   515 ---------------------------------------------YMKNNQVFVTYLFFYITVNL 534
                                                         :::|.:..:..:..:..:.:
Mouse   986 ELGSTGLKKRFGKKVMGPSPRLYTEALQEKKQSGFLAQKFKQYKRFVENYRRHIVCVTIFSAICI 1050

  Fly   535 CLFISRAIQYRASNGF--------------VIIARACGQCLNFNCAWVLVLMLRHSLTYLRGRGL 585
            .||..||..|    ||              :|::|.....::|..:::|:.|.|:.:|:||...|
Mouse  1051 GLFADRAYYY----GFASPPTDIEETTYVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFL 1111

  Fly   586 SSYLPLDHHVYLHKLTGITISVLSLIHTIMHLFNFSIIVIND--------PN--INAG------H 634
            :.|:|.|..|..|:...:...||:::|:..|..|..|..::.        ||  :|.|      :
Mouse  1112 NRYIPFDAAVDFHRWIAMAAVVLAVLHSAGHAVNVYIFSVSPLSLMACVFPNVFVNDGSKFPPKY 1176

  Fly   635 YTIGEWLLTDRPGLFGLIPGCANPTGVALLAILVVMFVCSQPFVRRKGSFEVFYWTHLLYVPFWI 699
            |.   |.....||:          |||.||.:|.:|:|.:....||. ||..|:.||.|||..::
Mouse  1177 YW---WFFETVPGM----------TGVLLLLVLAIMYVFASHHFRRH-SFRGFWLTHHLYVVLYV 1227

  Fly   700 LCLFHG-------PNFWKWFLLPGLVYIVERALRFIWMRGEHGKTYIS--SGLLLPSKVVHLVIK 755
            |.:.||       |:|..:||:|.::|..::.:..     ...|..||  ...||||.|.:|..:
Mouse  1228 LIIIHGSYALIQLPSFHIYFLVPAIIYGGDKLVSL-----SRKKVEISVVKAELLPSGVTYLQFQ 1287

  Fly   756 RPHHFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYRYFEREQQKL 820
            ||..|.::.|.:|.:....:...|:||||::||| .||.:.||||.||.||.||...:.
Mouse  1288 RPKTFEYKSGQWVRIACLDLGTNEYHPFTLTSAP-HEDTLSLHIRAVGPWTTRLREIYS------ 1345

  Fly   821 QQSGSSQEIPQHMHAIPTPSFMLLNEARNPAIAGERSATPQTDFLAKNLGVQAVPPVRPPRQNRK 885
                                                                  |||
Mouse  1346 ------------------------------------------------------PPV-------- 1348

  Fly   886 PAPGAPIDPPATGVNRIRSIKKTLQRTFSRKEAVDPKKGIPNGAFIADGEREDSNLKQRPLEKSI 950
                                                                             
Mouse  1349 ----------------------------------------------------------------- 1348

  Fly   951 SLPDISVKSKKRSRLKALRALGRSESESAFDEKRVRRARNNSVGLAYLSPQNKSLAQSFRYMRTK 1015
                                                                             
Mouse  1349 ----------------------------------------------------------------- 1348

  Fly  1016 PTIIAFKTPSMEEREHQVAAGEANGASPASRAEQGQLSSRMDSADKLQLARLSLSAEGASKPLED 1080
                                                                             
Mouse  1349 ----------------------------------------------------------------- 1348

  Fly  1081 QTQTGSPSRKSILRRPTFLRSLSASINNRTGGGGGGSTGSSTTNSGGKVTLDAGVMEIFIDGPYG 1145
                                              ||:.              |...::::|||:|
Mouse  1349 ----------------------------------GGTC--------------ARYPKLYLDGPFG 1365

  Fly  1146 APSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHSCPRCQFEWASEIPKSVMNLRKVDF 1210
            .........:.:||:|.|||||||||||:.::.:                 ....|.|..:|:.|
Mouse  1366 EGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFK-----------------SSMGSQMLCKKIYF 1413

  Fly  1211 FWINRDQRSFEWFVNLLSQLEIEQAELGGAMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEK 1275
            .|:.|.||.|||..:::.::|....      :..:.:|:|||...::.|::...|.:......:.
Mouse  1414 IWVTRTQRQFEWLADIIREVEENDC------QDLVSVHIYITQLAEKFDLRTTMLYICERHFQKA 1472

  Fly  1276 GKRDLITGLKTRTNAGRPNWDKVFKQLQA--QQKGKVTVFYCGPPQLAKTLRYKC------DQYG 1332
            ..|.|.|||::.|:.|||.::..|..||.  .|..|:.||.||||.:.|.:...|      |:..
Mouse  1473 LNRSLFTGLRSITHFGRPPFELFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRAH 1537

  Fly  1333 FAFRKECF 1340
            |....|.|
Mouse  1538 FVHHYENF 1545

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nox	NP_611116.2	EFh_HEF	222..425	CDD:355006	53/223 (24%)
		EF-hand motif	290..316	CDD:320075	3/28 (11%)
		FRQ1	331..462	CDD:444056	40/144 (28%)
		EF-hand motif	351..388	CDD:320075	15/37 (41%)
		EFh	365..425	CDD:238008	23/59 (39%)
		EF-hand motif	402..425	CDD:320075	7/22 (32%)
		PLN02844	<544..>816	CDD:215453	93/310 (30%)
		FNR_like	<1136..>1178	CDD:447143	18/41 (44%)
		NAD_binding_6	1158..1325	CDD:429792	53/168 (32%)
Duox2	NP_001349684.1	dual_peroxidase_like	38..594	CDD:188652
		EFh	824..885	CDD:238008	23/60 (38%)
		EFh	861..919	CDD:238008	14/57 (25%)
		Ferric_reduct	1081..1230	CDD:426438	47/162 (29%)
		NOX_Duox_like_FAD_NADP	1271..1545	CDD:99783	98/608 (16%)