DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Not1 and cnot1

DIOPT Version :9

Sequence 1:	NP_001286252.1	Gene:	Not1 / 5740252	FlyBaseID:	FBgn0085436	Length:	2511	Species:	Drosophila melanogaster
Sequence 2:	NP_001073420.1	Gene:	cnot1 / 448949	ZFINID:	ZDB-GENE-040915-1	Length:	2374	Species:	Danio rerio

Alignment Length:	2586	Identity:	1191/2586 - (46%)
Similarity:	1583/2586 - (61%)	Gaps:	313/2586 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 ALILASKPYAMQLFDSKPYITDSIYDILQFVKEHGLEADRSSLRHLFSVINFS-DLVPSVTVQLQ 75
            :|.|:...|.:.....|.| ..|..:|...|..||.||||..||.|||.::|| |...|.....|
Zfish     7 SLALSQISYLVDNLTKKNY-RASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQ 70

  Fly    76 AKLLGIQLERQLHSSSFVSNICYAFDQFFASNQKQSLKPVAVADLVGQVARLTGINKVCECVFAL 140
            .:.|..:....:...:|:|.:|||.|.  ..:.::||||  ...|..|::::..::||.|.:..|
Zfish    71 TQFLIQECVSLITKPNFISTLCYAIDN--PLHYQKSLKP--SPHLFTQLSKVLKLSKVQEVILGL 131

  Fly   141 ALTHSSYTELKHSARNNLKGSLSELIDSYLGNKGTGPADSGLREISFDLLQYLLCCL-------S 198
            ||::||..:|:..|...:|..|.:|:.||:.....|..:.|.::|:.::|..||..|       |
Zfish   132 ALSNSSNADLRGFAAQFVKQKLPDLLRSYVDADLGGNQEGGFQDIAIEVLHLLLSHLLFGQKGSS 196

  Fly   199 EYVQPQVEAQFLIKLREEFPRQAVPLVLAPFLYGSTTATIAGAGASETDAEAEATTNSNSSSFEA 263
            ...|.|::| ||..|..:||::..|:||||.||
Zfish   197 GVGQEQIDA-FLKTLCRDFPQERCPVVLAPLLY-------------------------------- 228

  Fly   264 DALNEVGIEDIYDHLSEIIFTNQGKNN--IMDTSWINLILEIGYEFTSSVEECKNHLCSRERERA 326
                    .|..|.|.:.|..:.|..|  :|::|..:.:.|:||.|.:|:|||:|.:.  :....
Zfish   229 --------PDKRDILMDRILPDSGDLNKTMMESSLADFMQEVGYGFCASLEECRNIIL--QYGVR 283

  Fly   327 ELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPGSSN 391
            |:.:..||:::|:|.|.||.|                        |.|.|...||
Zfish   284 EVTASQVARVLGMMARTHSGL------------------------SDGISLQTIT---------- 314

  Fly   392 NNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRIGLE 456
             |....|...||.|||   ::::|.|..:|.:..:|||.|.||:|:|..|||||.|:::|..||:
Zfish   315 -NPVGGGGIWSDGKDK---SDSSQAWNVEVLIDVVKEVNPNLNFKEVTYELDHPGFLIRDSKGLQ 375

  Fly   457 LLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKTAPD 521
            :::..::...|..:|| .:.|||.|.:.|||||.|...|.:|::|.|||......|:|.||..|:
Zfish   376 IVVYGIQRGLGMEVFP-VDLIYRPWKHAEGQLSFIQHSLLSPEVFCFADNPCHTVAIDTLKAPPE 439

  Fly   522 ADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQDLFN 586
            .||:||::||||.|||.||.:::.|:|.||.:||.||.::|||:|.||||..|.....||.:|.:
Zfish   440 DDNREIATWKSLDLVESLLRLSEVGHYEQVKQLFSFPIKHCPDMLVLALLQISTSWHTLRHELIS 504

  Fly   587 QLIPTFLGNHPNSNVILASAW--SSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQDLKA 649
            .|:|.||||||||.:||..||  ...:..:|..||::|:|||:||.::||.|||||||:|||||:
Zfish   505 TLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHSMAEWYMRGEQYDQAKLSRILDVAQDLKS 569

  Fly   650 LSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAKVPE 714
            ||.|||...|.|:||||.||||||||||:||||||||||||||:||.:..|.||||.::....||
Zfish   570 LSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACVTFLKRRCPSIMGGLAPE 634

  Fly   715 -DQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPGLVP 778
             ||  ||.|||.|||:.||:.|||:|..:..| |:.|.|:.|.||.:.:.||||
Zfish   635 KDQ--PKSAQLPPETLATMLACLQSCAGSVSQ-ELSETILTMVANCSNVMNKAR----------- 685

  Fly   779 PPPPTILRGHRGMDLPGGIVPPPPQQPFSGNLNAQMFGP-GMDPLTNMSN--------------- 827
            .|||.:|              |..:.|.:.:|:|  ..| .||||:.|.:
Zfish   686 QPPPGVL--------------PKGRAPSTSSLDA--ISPVQMDPLSAMGSLSLGVSSTSHTPSMQ 734

  Fly   828 ---NLAGLNLSGPNG-AFNFGNMLTSPSRLMTPGANPYPLNLMQMPQAPPPPNVGNLGRMLPGGP 888
               :|.|...|.|.. |..|.|:.........||.||    |....|.|...::..:|..| |.|
Zfish   735 GFPSLQGSAFSNPQSPAKAFSNLPNPNPSTAFPGINP----LSSQLQGPLSTSLSGIGSGL-GMP 794

  Fly   889 ----------QQQTP--TPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSID 941
                      :..||  .|........:.|..:.....|||::||.||||||||||.|:||:|:|
Zfish   795 TVSSDVFSARKMSTPGLNPPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVD 859

  Fly   942 EVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVAL 1006
            |||::|||||:|:.:||:|||.|||||||||||||.|||:|||.|||.||||||::.|| |::||
Zfish   860 EVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLV-TYMAL 923

  Fly  1007 GLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYVEYGM 1071
            ||:||.||:|||||.|||:||||:.|||||:.||..|.:||:|:.||.||..||.||.:|:|||.
Zfish   924 GLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASIAHFLQFPHHLQEYIEYGQ 988

  Fly  1072 HGQEPPPQKLIGLSNTIPSAIS-------SGPGTEPIYRNSSMLGNMPAATPGS----------- 1118
            ..:: ||.|:.| |.|.|.:::       |.|...|....:|.|......|..:
Zfish   989 QSRD-PPVKMQG-SITTPGSLALAQAQAQSQPPKAPQPGQASTLVTTATTTTTAAKTTTITRPTA 1051

  Fly  1119 -GPKSNAAVSHATRMKSIANATNIDTLLVA-NQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCD 1181
             |||.:...|        .|.||||||||| :|.|::..|||.||:|.|||||||||.|:.||.:
Zfish  1052 VGPKKDVPPS--------INTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMSQKVE 1108

  Fly  1182 EIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDK 1246
            |:||.:.:|:.||::||||:||.|:|.|||:||.||||.|||.|..:.|..||.|||||||.|||
Zfish  1109 ELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFVKMVLNETYRNIKVLLTSDK 1173

  Fly  1247 GVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESS 1311
            ...|||||||||||||||||:||.:|:|||..||:|||||.|||.|||||||:|||||||:||||
Zfish  1174 AAANFSDRSLLKNLGHWLGMITLAKNKPILYTDLELKSLLLEAYVKGQQELLYVVPFVAKVLESS 1238

  Fly  1312 AKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPNRTHR 1376
            .:|.|||..||||||||.||||||||.|||||||||||||||.|::::..|:....|:|.::...
Zfish  1239 LRSVIFRPQNPWTMGIMNVLAELHQEHDLKLNLKFEIEVLCKNLSMDITDLKPGNLLRDKDKLKT 1303

  Fly  1377 IEEQMSQPK-----PKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNA 1436
            :|||:|.||     |::|.|:.:..::|
Zfish  1304 LEEQLSAPKKETKPPEELLPIVTTDSVP------------------------------------- 1331

  Fly  1437 AAMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLH 1501
                  ...|.|||.        |.::..|..||.|::||.|:||.....:...:.:..|.|.|.
Zfish  1332 ------FTAAPSTPA--------TTTACTATGPPTPQFSYHDINVYALAGLAPHININVNIPLLQ 1382

  Fly  1502 ANPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLA 1566
            |:|.:|..|..|:||.:.:.:.|:|||||:||..|.|||:|||||||::|:.||.|||.|:|||.
Zfish  1383 AHPQLKQCVRPAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESHMRVAAHHMMRNLT 1447

  Fly  1567 AGMAMITGKDEIARAISQNLHKALLSGLNG-MPSMAE-IQAAAMQLASENVELVCAFIQKTSAEK 1629
            |||||||.::.:..:|:.||..:..:.|.. .|...| ::.||.::|.:|.||.|.|||||:.||
Zfish  1448 AGMAMITCREPLLMSIATNLKNSFAAALRAPTPQQREMMEEAAARIAQDNCELACCFIQKTAVEK 1512

  Fly  1630 AAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSI 1694
            |..|:|:||:|:||.||.||:||.|:.|..:||||.||:||.:|:|||.......|||.||||::
Zfish  1513 AGPEMDKRLATEFELRKHARQEGRRYCDPMVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNV 1577

  Fly  1695 PGFQQMSDRDIALFVPKPTDLSQPNVF-----------ANDDSMVYGELASKMEAFMNTAIGVPT 1748
            ||           |:|. .|||||..|           .:|.:.:|.:..|.:|..::.......
Zfish  1578 PG-----------FLPS-NDLSQPTGFLAQPMKQQAWPTDDVAHIYEKCISDLEQHLHAIPPALA 1630

  Fly  1749 LQIQASKMHMLLNALIATRRLRDQESAFNLLTRAVEGL---TEGLVNMHENMEQMKMYQNIHLRI 1810
            :..|...:..||.|::..|..||..:|..||.:|||||   |.|     .:.|.:..|:..||.:
Zfish  1631 MNPQTQAIRSLLEAVVMARNSRDGIAALGLLQKAVEGLLDATSG-----ADPELLLSYRECHLLV 1690

  Fly  1811 LGLLNN--SFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNY 1873
            |..|.:  ::|.....:.:|:|..:.|:|.:|||||...||.:|.||:.|:|..|...|:||.||
Zfish  1691 LKALQDGRAYGPQWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNY 1755

  Fly  1874 VAISFGIALLERLIMDDRVINIVSDNEFMATVELLGRLTQH-RHRYPECIVNAIDTLWSGNFNSS 1937
            :|::|.:.|::.|::|:|.::.:::.:...|:|.|.|.:.| |...||.:...:|.:.| |:.:.
Zfish  1756 MAVAFAMQLVKLLLVDERSVSHITEADLFHTIETLMRTSAHSRANAPEGLPQLMDVVRS-NYEAM 1819

  Fly  1938 SDYSPFNGNDRYLSGASHYIHSGMHHVRSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDA- 2001
            .        ||:..|.:..:|||:.  ::.:.||||||:||.|:||::||.||  .:..:.||:
Zfish  1820 I--------DRHHGGPNFMMHSGIS--QASEYDDPPGLREKAEYLLREWVNLY--HSAAAGRDST 1872

  Fly  2002 RNFGAFVQKMNTYGILKTDDLITRFFRQATHICTDVVYRMFAEPSLPINQ---AKNKIFQWIDAF 2063
            :.|.|||.:|:..||||||||||||||..|.:|.::.||..||...|...   .:.|.:..:|||
Zfish  1873 KAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHPTTSPAIIRAKCYHNLDAF 1937

  Fly  2064 VHLIAMLVRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTA 2128
            |.|||:||:|||||.|..|||||||||||||:|.||:||::|...|||:.|||.|:||.:||...
Zfish  1938 VRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLIQDHDVRQTEFQQLPYHRIFIMLLLELNAP 2002

  Fly  2129 DVILESLMHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQ 2193
            :.:||::....::||..|:|:|.|:.||||.:|||||||||:|:.|:|...|.|||||:|||||.
Zfish  2003 EHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLI 2067

  Fly  2194 DLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILS 2258
            ||||||||||||.||.||:|:||||||||||||||||||||||||:||||.|||||:|:||:|||
Zfish  2068 DLFKYLAPFLRNVELNKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILS 2132

  Fly  2259 AFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRG 2323
            ||||||||||||||||||||||:.:.||::|::: ..:.|:.||||||||||.|:|||||||||.
Zfish  2133 AFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNF-TGVMPSQFKKDLDSYLKTRSPVTFLSELRS 2196

  Fly  2324 HLQVTSEPGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGR 2388
            :|||::|||.|||:.|:||||.||||||||.|.||...|:.|.|.||||||||||||||||||||
Zfish  2197 NLQVSNEPGNRYNIQLINALVPYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGR 2261

  Fly  2389 YLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIE 2453
            |||||||||||||||||||||||.:|:||||||:|||||||||||||||||||||||||||||||
Zfish  2262 YLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANAEAIQEQITRVLLERLIVNRPHPWGLLITFIE 2326

  Fly  2454 LIKNPIYKFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMPVVDGEG 2504
            |||||.:|||.||||||||||.|||:|||:.|:.:....|     |::|.|
Zfish  2327 LIKNPAFKFWSHDFVHCAPEIEKLFQSVAQCCMGQKQAQQ-----VMEGTG 2372

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Not1	NP_001286252.1	CNOT1_HEAT	582..737	CDD:293027	95/157 (61%)
		CNOT1_TTP_bind	906..1079	CDD:293026	110/172 (64%)
		CNOT1_CAF1_bind	1155..1373	CDD:293024	148/217 (68%)
		DUF3819	1506..1649	CDD:289602	71/144 (49%)
		Not1	2129..2478	CDD:281978	265/348 (76%)
cnot1	NP_001073420.1	LXXLL	153..157		1/3 (33%)
		LXXLL	181..185		1/3 (33%)
		LXXLL	223..227		3/3 (100%)
		CNOT1_HEAT	500..656	CDD:293027	95/157 (61%)
		LXXLL	570..574		2/3 (67%)
		Forkhead_N	698..805	CDD:254796	27/113 (24%)
		CNOT1_TTP_bind	812..996	CDD:293026	112/185 (61%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1009..1060		9/50 (18%)
		CNOT1_CAF1_bind	1080..1305	CDD:293024	148/224 (66%)
		Interaction with CCR4-NOT complex catalytic subunits. /evidence=ECO:0000250	1082..1604		283/584 (48%)
		DUF3819	1387..1532	CDD:289602	71/144 (49%)
		LXXLL	1638..1642		0/3 (0%)
		LXXLL	1940..1944		3/3 (100%)
		Not1	1996..2351	CDD:281978	267/355 (75%)
		LXXLL	2094..2098		3/3 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170590891
Domainoid	1	1.000	572	1.000	Domainoid score	I213
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H9453
Inparanoid	1	1.050	2034	1.000	Inparanoid score	I46
OMA	1	1.010	-	-		QHG54022
OrthoDB	1	1.010	-	-		D42530at2759
OrthoFinder	1	1.000	-	-		FOG0003737
OrthoInspector	1	1.000	-	-		oto40413
orthoMCL	1	0.900	-	-		OOG6_101511
Panther	1	1.100	-	-	LDO	PTHR13162
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R833
SonicParanoid	1	1.000	-	-		X2594
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	14	13.940