DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Not1 and CNOT1

DIOPT Version :9

Sequence 1:	NP_001286252.1	Gene:	Not1 / 5740252	FlyBaseID:	FBgn0085436	Length:	2511	Species:	Drosophila melanogaster
Sequence 2:	NP_057368.3	Gene:	CNOT1 / 23019	HGNCID:	7877	Length:	2376	Species:	Homo sapiens

Alignment Length:	2579	Identity:	1196/2579 - (46%)
Similarity:	1594/2579 - (61%)	Gaps:	297/2579 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 ALILASKPYAMQLFDSKPYITDSIYDILQFVKEHGLEADRSSLRHLFSVINFS-DLVPSVTVQLQ 75
            :|.|:...|.:.....|.| ..|..:|...|..||.||||..||.|||.::|| |...|.....|
Human     7 SLALSQISYLVDNLTKKNY-RASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQ 70

  Fly    76 AKLLGIQLERQLHSSSFVSNICYAFDQFFASNQKQSLKPVAVADLVGQVARLTGINKVCECVFAL 140
            .:.|..:....:...:|:|.:.||.|.  ..:.::||||  ...|..|::::..::||.|.:|.|
Human    71 TQFLIQECALLITKPNFISTLSYAIDN--PLHYQKSLKP--APHLFAQLSKVLKLSKVQEVIFGL 131

  Fly   141 ALTHSSYTELKHSARNNLKGSLSELIDSYLGNKGTGPADSGLREISFDLLQYLLCCL-------S 198
            ||.:||.::|:..|...:|..|.:|:.||:....:|..:.|.::|:.::|..||..|       .
Human   132 ALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAF 196

  Fly   199 EYVQPQVEAQFLIKLREEFPRQAVPLVLAPFLYGSTTATIAGAGASETDAEAEATTNSNSSSFEA 263
            ...|.|::| ||..||.:||::..|:||||.||
Human   197 GVGQEQIDA-FLKTLRRDFPQERCPVVLAPLLY-------------------------------- 228

  Fly   264 DALNEVGIEDIYDHLSEIIFTNQG--KNNIMDTSWINLILEIGYEFTSSVEECKNHLCS---RER 323
                    .:..|.|.:.|..:.|  ...:|::|..:.:.|:||.|.:|:|||:|.:..   ||.
Human   229 --------PEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREV 285

  Fly   324 ERAELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPG 388
            ..|:     ||:::|:|.|.||.|.|   .:|..:...||.|
Human   286 TAAQ-----VARVLGMMARTHSGLTD---GIPLQSISAPGSG----------------------- 319

  Fly   389 SSNNNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRI 453
                       ..||.|||.:..:| .||..:|.:..|||:.|.||:|:|..|||||.|.::|..
Human   320 -----------IWSDGKDKSDGAQA-HTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSK 372

  Fly   454 GLELLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKT 518
            ||..::..::...|..:|| .:.|||.|.:.|||||.|...|.||::|.|||:.....|.|:||.
Human   373 GLHNVVYGIQRGLGMEVFP-VDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKA 436

  Fly   519 APDADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQD 583
            .|:.||:||::||||.|:|.||.:|:.|.|.||.:||.||.::|||:|.||||..:.....||.:
Human   437 PPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHE 501

  Fly   584 LFNQLIPTFLGNHPNSNVILASAW--SSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQD 646
            |.:.|:|.||||||||.:||..||  ...:..:|..||:||:|||:||.::||.|||||||:|||
Human   502 LISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQD 566

  Fly   647 LKALSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAK 711
            |||||.|||...|.|:||||.||||||||||:||||||||||||||:||.:..|.||||.::...
Human   567 LKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGL 631

  Fly   712 VPE-DQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPG 775
            .|| ||  ||.|||.|||:.||:.|||.|..:..| |:.|.|:.|.||.:.:.||||   |..||
Human   632 APEKDQ--PKSAQLPPETLATMLACLQACAGSVSQ-ELSETILTMVANCSNVMNKAR---QPPPG 690

  Fly   776 LVP---PP---------PPTI--LRGHRGMDLPGGIVP--------PP--------PQQPFSGNL 810
            ::|   ||         |..|  |.|...:.:.|...|        ||        ||.|
Human   691 VMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSP----- 750

  Fly   811 NAQMFGPGMDP-LTNMSNNLAGLNLSGPNGAFNFGNML-TSPSRLMTPGANPYPLNLMQMPQAPP 873
             |:.|.|...| .|...:.:.||:...|.|....|::. .....|..|..|..|.
Human   751 -AKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPF---------- 804

  Fly   874 PPNVGNLGRML-PGGPQQQTPTPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPT 937
                  :.|.| ..|..|.|...:...|:..:.|..:.....|||::||.||||||||||.|:||
Human   805 ------VQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPT 863

  Fly   938 LSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPT 1002
            :|:||||::|||||:|:.:||:|||.|||||||||||||.|||:|||.|||.||||||::.|| |
Human   864 MSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLV-T 927

  Fly  1003 FVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYV 1067
            ::||||:||.||:|||||.|||:||||:.|||||:.||..|.:||:|:.||.||..||.||.:|:
Human   928 YMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYI 992

  Fly  1068 EYGMHGQEPPPQKLIGLSNTIPSAISSGPGTEPIYRNSSMLGNMPAATPGSGPKSNAAVSHATR- 1131
            |||...:: ||.|:.| |.|.|.:|:        ...:.....:||..|.:|..|....:..|.
Human   993 EYGQQSRD-PPVKMQG-SITTPGSIA--------LAQAQAQAQVPAKAPLAGQVSTMVTTSTTTT 1047

  Fly  1132 -MKSIA-----------------NATNIDTLLVA-NQEEKVTVPPEPVQDKTAFIFNNLSQLNIP 1177
             .|::.                 |.||||||||| :|.|::..|||.:|:|.|||||||||.|:.
Human  1048 VAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMT 1112

  Fly  1178 QKCDEIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLL 1242
            ||.:|:||.:.:|:.||::||||:||.|:|.|||:||.||||.|||.|.|:.|..||.|||||||
Human  1113 QKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLL 1177

  Fly  1243 RSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKI 1307
            .|||...|||||||||||||||||:||.:|:|||..|||:||||.|||.|||||||:|||||||:
Human  1178 TSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKV 1242

  Fly  1308 LESSAKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPN 1372
            ||||.:|.:||.||||||.||.||||||||.|||||||||||||||.|.|::.:|:....|||.:
Human  1243 LESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKD 1307

  Fly  1373 RTHRIEEQMSQPKPKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNAA 1437
            |...::||:|.||....:|                            :::||..::.
Human  1308 RLKNLDEQLSAPKKDVKQP----------------------------EELPPITTTT-------- 1336

  Fly  1438 AMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLHA 1502
                    .::||.        |:::..|..||:|:|||.|:||.:...:...:.|.|..|...|
Human  1337 --------TSTTPA--------TNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQA 1385

  Fly  1503 NPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAA 1567
            :|.:|..|..|:||.:.:.:.|:|||||:||..|.|||:|||||||::|:|||.|||.|:|||.|
Human  1386 HPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTA 1450

  Fly  1568 GMAMITGKDEIARAISQNLHKALLSGL-NGMPSMAEI--QAAAMQLASENVELVCAFIQKTSAEK 1629
            ||||||.::.:..:||.||..:..|.| ...|...|:  |||| |||.:|.||.|.|||||:.||
Human  1451 GMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAA-QLAQDNCELACCFIQKTAVEK 1514

  Fly  1630 AAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSI 1694
            |..|:|:||:|:||.||.||:||.|:.|..:||||.||:||.:|:|||.......|||.||||::
Human  1515 AGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNV 1579

  Fly  1695 PGFQQMSDRDIALFVPKPTD-LSQP--NVFANDD-SMVYGELASKMEAFMNTAIGVPTLQI--QA 1753
            |||...:|      :.:||. |:||  ..:|.|| :.:|.:..:::|..::..  .|||.:  ||
Human  1580 PGFLPTND------LSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAI--PPTLAMNPQA 1636

  Fly  1754 SKMHMLLNALIATRRLRDQESAFNLLTRAVEGLTEGLVNMHENMEQMKMYQNIHLRILGLLNN-- 1816
            ..:..||..::.:|..||..:|..||.:|||||.:.......::  :..|:..||.:|..|.:
Human  1637 QALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADL--LLRYRECHLLVLKALQDGR 1699

  Fly  1817 SFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNYVAISFGIA 1881
            ::|:|...:.:|:|..:.|:|.:|||||...||.:|.||:.|:|..|...|:||.||:|::|.:.
Human  1700 AYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQ 1764

  Fly  1882 LLERLIMDDRVINIVSDNEFMATVELLGRLTQH-RHRYPECIVNAIDTLWSGNFNSSSDYSPFNG 1945
            |::.|::|:|.:..|::.:...|:|.|.|:..| |...||.:...::.:.| |:.:..
Human  1765 LVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRS-NYEAMI------- 1821

  Fly  1946 NDRYLSGASHYIHSGMHHVRSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDA-RNFGAFVQ 2009
             ||...|.:..:|||:.  ::.:.||||||:||.|:||::||.||  .:..:.||: :.|.|||.
Human  1822 -DRAHGGPNFMMHSGIS--QASEYDDPPGLREKAEYLLREWVNLY--HSAAAGRDSTKAFSAFVG 1881

  Fly  2010 KMNTYGILKTDDLITRFFRQATHICTDVVYRMFAE----PSLPINQAKNKIFQWIDAFVHLIAML 2070
            :|:..||||||||||||||..|.:|.::.||..||    |:......:.|.:..:||||.|||:|
Human  1882 QMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALL 1946

  Fly  2071 VRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTADVILESL 2135
            |:|||||.|..|||||||||||||:|.|::||::|...|||:.|||.|:||.:||...:.:||::
Human  1947 VKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETI 2011

  Fly  2136 MHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLA 2200
            ....::||..|:|:|.|:.||||.:|||||||||:|:.|:|...|.|||||:|||||.|||||||
Human  2012 NFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLA 2076

  Fly  2201 PFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMR 2265
            |||||.||.||:|:||||||||||||||||||||||||:||||.|||||:|:||:||||||||||
Human  2077 PFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMR 2141

  Fly  2266 LPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRGHLQVTSE 2330
            |||||||||||||||:.:.||::|::: ..:.|..||||||||||.|:||||||:||.:|||::|
Human  2142 LPDPFTPNLKVDMLSEINIAPRILTNF-TGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNE 2205

  Fly  2331 PGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAI 2395
            ||.|||:.|:||||:||||||||.|.||...|:.|.|.|||||||||||||||||||||||||||
Human  2206 PGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAI 2270

  Fly  2396 ANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIY 2460
            ||||||||||||||||.:|:||||||:||||||||||||||||||||||||||||||||||||.:
Human  2271 ANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAF 2335

  Fly  2461 KFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMPVVDGEG 2504
            |||:|:||||||||.|||:|||:.|:.:....|     |::|.|
Human  2336 KFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQ-----VMEGTG 2374

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Not1	NP_001286252.1	CNOT1_HEAT	582..737	CDD:293027	97/157 (62%)
		CNOT1_TTP_bind	906..1079	CDD:293026	110/172 (64%)
		CNOT1_CAF1_bind	1155..1373	CDD:293024	149/217 (69%)
		DUF3819	1506..1649	CDD:289602	78/145 (54%)
		Not1	2129..2478	CDD:281978	263/348 (76%)
CNOT1	NP_057368.3	LXXLL	153..157		1/3 (33%)
		LXXLL	181..185		1/3 (33%)
		LXXLL	223..227		3/3 (100%)
		CNOT1_HEAT	500..650	CDD:318600	94/151 (62%)
		LXXLL	570..574		2/3 (67%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	720..768		12/53 (23%)
		Interaction with ZFP36	800..1015		124/233 (53%)
		Not1	<840..2369	CDD:331109	856/1621 (53%)
		Interaction with CNOT6, CNOT6L, CNOT7 and CNOT8. /evidence=ECO:0000269\|PubMed:22977175	1090..1605		288/573 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1315..1352		11/88 (13%)
		LXXLL	1639..1643		0/3 (0%)
		LXXLL	1942..1946		3/3 (100%)
		LXXLL	2096..2100		3/3 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165155716
Domainoid	1	1.000	571	1.000	Domainoid score	I228
eggNOG	1	0.900	-	-		E1_COG5103
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H9453
Inparanoid	1	1.050	2042	1.000	Inparanoid score	I51
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54022
OrthoDB	1	1.010	-	-		D42530at2759
OrthoFinder	1	1.000	-	-		FOG0003737
OrthoInspector	1	1.000	-	-		oto91655
orthoMCL	1	0.900	-	-		OOG6_101511
Panther	1	1.100	-	-	LDO	PTHR13162
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2594
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	15	14.770