Protein Alignment RhoGAP1A and BEM2

DIOPT Version :10

• Sequence 1: NP_001284725.1
• Gene: RhoGAP1A / 5740235
• FlyBaseID: FBgn0025836
• Length: 1404
• Species: Drosophila melanogaster
• Sequence 2: NP_011082.3
• Gene: BEM2 / 856899
• SGDID: S000000957
• Length: 2167
• Species: Saccharomyces cerevisiae
• Alignment Length: 1753
• Identity: 343/1753 - (19%)
• Similarity: 590/1753 - (33%)
• Gaps: 557/1753 - (31%)

  Fly     7 FQRLWMQRFPQSSLSDAWEQDVRASLERHKVRINELSKELEQETLYVEYLERLLSD--------- 62
            |.::|..:|..|.|.|:      ..:.|..|.:|.|....:|.   :.:|.|||..
Yeast   607 FDKVWAPKFDYSLLYDS------KFINRRIVSLNPLVFNNDQN---IHFLGRLLISHLFPTNPEF 662

  Fly    63 ------------VERYRVSG------GDPKALFEAASGSCASSNNSNCSSTTNGATQEESNTNLD 109
                        ::::...|      ||..:....|:..|:.   ....|::.....::|...:.
Yeast   663 SKKVTPKVRAELLDKWVQIGCRFEHLGDMVSWLAVATIICSI---PVLRSSSWKYVPDQSLKTIF 724

  Fly   110 KDFKSSLNLRESPSSTGAKDKDRLSLNPDNKYTTHTNA-VIGGIGSEKVSGPLHSTQITADGKRT 173
            ||:..::...|....|.........|.|.|.....|.| ||...|...:    |:..:.:|.|..
Yeast   725 KDWVPTIIQLERRQRTSKSTSSVFILAPPNLDDDFTRANVISYFGDLLI----HADDLPSDTKFK 785

  Fly   174 GELYLKREEDKCKDSNSEDTDGALQQCITELAASLKAQPDNVAGGRLENDVDIDKPLRKSTSQYV 238
               ||:::.::.|:        |..:....|.|                   ||....|:.|
Yeast   786 ---YLEKKINRTKN--------AFHKWQQRLQA-------------------IDSTRHKTNS--- 817

  Fly   239 TVIQVKEAKDKESADGTT-------------SSSGIGEPQQQPTPP-------ST---------- 273
                .:..:|.:|.:...             :..||.:...|..||       ||
Yeast   818 ----TENVRDNDSPNNVVYQLWKFHLSQPPLNIEGIMKLSVQHEPPIIDQKAYSTIGSQRSALVT 878

  Fly   274 -----------FSRYSEATAPPLSPSLTSYAAHVEAKKKMPPRPPPKNIRRVEPPTIPSQQLSSS 327
                       |..||      |.|..|...|..:||     .|||::..|:      |:.||.|
Yeast   879 GSYLPILFNELFPNYS------LFPKNTLVGAASDAK-----LPPPRSSARL------SKSLSIS 926

  Fly   328 PKRETYMINTMPSSSS----GSLCIDSLGNSLERN----IKPSD--ILRQKSSDNLDSKYSAKRK 382
                    ..:|.:|:    |||..|::.:..:.|    :...|  ::::.|     ||.|.|::
Yeast   927 --------EPIPIASNSHTMGSLTDDAMSSKNDNNKVTGVGKIDGPVIKEMS-----SKQSNKQR 978

  Fly   383 TTPELSKFVNVNNP--ELMEELGRKMVSKADS--------LEQTRRNDSSPSGCGSLGRTSSTPG 437
            ....:....|::..  .:.:||..|.|...|.        :|..:|.....|   .|....:||.
Yeast   979 LLKSVRDVFNIDMDVFHISDELVFKSVYDNDGKSRPASMVIETPKRFSQHSS---MLINNPATPN 1040

  Fly   438 RSLTPGRTGVGASPTGSLSKT----------GKLAMADSSS-------TSSLERRSCLQAVDADI 485
            :.:..     ....||.||||          |:::.:...|       :||||:...|..:.::|
Yeast  1041 QKMRD-----SLDTTGRLSKTLENMDFFNNIGQVSDSLKESIIRVVLKSSSLEKIFDLLVLTSNI 1100

  Fly   486 GTE-------------SRESGANQRSVGS----------------------------KESLTSQG 509
            .::             .|:.|.:.|.:..                            |...|:..
Yeast  1101 FSKLVDTKDLENYYYHQRQRGHSTRGLSDDNIGLLDYAFVKLTMDNDIFTETFFNTYKSFTTTTT 1165

  Fly   510 ISEVQIKSYESVSSLS---------SESAKAVASQQSAAAGGALAECE-PYYDSVPLDNGDGEYV 564
            :.|...|.|....|.|         |:.:|...::.:.....:|.:.. |.:|....|:.:...:
Yeast  1166 VLENMAKRYVGAKSCSVSISKILDRSDDSKMKINEDTNLVSSSLYDQNFPVWDMKVTDDENINLI 1230

  Fly   565 FIKPGRTGS------------SSSRDDLSTPGSSILPLPNVADPES----PGRTSNYVNIDFFLQ 613
            ::...:.|:            |...|||.. .|::|.:..:.:.|.    |.|.:|
Yeast  1231 YMAKIQIGAAEAILHLVKNHYSDFTDDLCN-NSTLLDIIKIMEQEVSTEWPTRIAN--------- 1285

  Fly   614 QGIETRSSSLDSDGEYEGPPILRTIS---HDDQTTTQTTPGAIRKNLVSAILVCGNARGAKIRSI 675
               .....||..:...|...:|.|::   |..::       |.:|.|...|.|  |....:|..|
Yeast  1286 ---SKLQKSLPENFVIETENLLTTLTDLFHGIKS-------AYQKQLYRPIGV--NRTQKRITDI 1338

  Fly   676 LSSIIQSETIYVECLNKMMQYKKAIHATLTTSQPVIR--EEEENTIFFKIDE-LYDLHTGFLSDL 737
            |:|.   .|.....||.::.       ..:.|..:||  ::..:|.:..|.| :|.| ..|:|..
Yeast  1339 LNSF---NTFSFTDLNNIID-------DPSFSDDMIRSFQKLHSTNYEDILEWIYQL-DNFISKK 1392

  Fly   738 KTIVSHEGGDVLIGEPFRRLAEMFDLYSAFLHNYKNAIETVKKCSANNPQFKKI----------- 791
            ..:||.:...||..|  ..|.....|.|.|  ||....::.|..   ||.:.::
Yeast  1393 FNLVSKKDWIVLFQE--LELLSKESLVSFF--NYPLHFKSSKLI---NPGYLQLHEFEISNLFTW 1450

  Fly   792 VSTIVLNLQT-EQSLTLEDLLHKPVARVQINALVFNDLLRETPNAHPDHQPLRQAQKIIQMFLNQ 855
            :||::|.... .:||..|.|.......::::..:....:.|..|.:..........|:|...||.
Yeast  1451 ISTLILKDDNGTESLFFEKLPQSIKLLIKLHTSLTTFFVMEISNVNKSSSERLTTCKVILQILNY 1515

  Fly   856 FNVVNQRLPIESNRNLRRMVRNSFIVELVDGHRKLRHLFLFNDVIACAKYKALGRDRIDYELKWF 920
            ....|..|.:..:.            |....|....|:..|  :.....:..:..:..:|||.|.
Yeast  1516 IRWKNGSLDLFDSE------------EDESPHAICPHIPAF--IETAIAHAIISPESRNYELSWI 1566

  Fly   921 IPLKDVSIYEEADPAVELKESSPANISQVKRNLRSVRDQLA------------------------ 961
                         .|.| |.|.|...:|   ||||:.:.|.
Yeast  1567 -------------KASE-KLSDPTKGTQ---NLRSISNVLEKIDDIHIKRFIEIDDVFSKNCKNL 1614

  Fly   962 --------------------MEVANSGSGAFRSGDKYRRKLADLESQLVLATPN-----LVLRLG 1001
                                |.:.||....|   || ||.:.::.|.::...||     |.:.:.
Yeast  1615 CPCPGWFISRLLEISQFVPNMSITNSKLINF---DK-RRFVNNIISNVLDLIPNEREFPLDIEMS 1675

  Fly  1002 NKANNKTITF--FLSSDFE--------RTQWIDSILSLKQKCNLPG------ANTI-------NS 1043
            ::..:|..||  .|.::||        :|:.:....::.::....|      .|.|       ..
Yeast  1676 DENPSKRTTFGRILFNNFEDVNKVYRKKTKKVSESEAISERFQEQGVFNEILVNEIEKIKREARK 1740

  Fly  1044 LEVT------------------------------------AFIVAMQKGMKTEMGSYLMRN---- 1068
            |||.                                    ::.:....|....:||.:..|
Yeast  1741 LEVLLDQEKILKNSAALHQAVPKKNRKSVIISGTHSDNDHSYNINKNTGQTPSLGSVMESNNSAR 1805

  Fly  1069 ----------TNDESLLVGDLYMGVHGLEGLEQANDLYICVEVDSYGHYFRKATTKKICRSQTPL 1123
                      ||..|::....:.||.                 ...|.:||:..:.....:.:..
Yeast  1806 NRRDSRASFSTNRSSVVSNSSHNGVS-----------------KKIGGFFRRPFSIGGFNTSSSN 1853

  Fly  1124 WNESFMLELEGSQNVRIL-----------LYEAKERPLLKA----------------------KH 1155
            ::.:.:|..|.|.|..||           |::.|....||.                      |.
Yeast  1854 YSLNSILSQEVSSNKSILPSILPEVDSMQLHDLKPSYSLKTFEIKSIMEIINHRNIPAYYYAFKI 1918

  Fly  1156 ILKLSLSWLTETTQPKSIKLTETLE-LGCSFRF-IPGELFRGSTKPGALFGAKMSQVLKREKRDI 1218
            :::....:|.:|.  .|..|||.:: :..|.|| ...:.::|.|. ..:||..:..|.:||...|
Yeast  1919 VMQNGHEYLIQTA--SSSDLTEWIKMIKASKRFSFHSKKYKGKTH-NKIFGVPLEDVCERENTLI 1980

  Fly  1219 PFIIGACIREVERRGMLEVGCYRVSGSASDLAKLKKAFESDAYEAEQLLREVD----IHSVTGIL 1279
            |.|:...:.|:|.||:.|||.||:.||...:..||.||:.:.........|.|    ::::.|..
Yeast  1981 PTIVVKLLEEIELRGLDEVGLYRIPGSIGSINALKNAFDEEGATDNSFTLEDDRWFEVNAIAGCF 2045

  Fly  1280 KTFLRELPEALFTDQLYPRFFD---TFSAFSNNNESTR--INELLKVFEELPQANKASITSILDH 1339
            |.:|||||::||:..:...|.|   .:.|.:..||..:  :||||   ::||.....::..|:.|
Yeast  2046 KMYLRELPDSLFSHAMVNDFTDLAIKYKAHAMVNEEYKRMMNELL---QKLPTCYYQTLKRIVFH 2107

  Fly  1340 LIRVHEKETDNKMSLHNLAMVFGPTLLRPGQTQVKQKDPLAASTVDVMAQAGILYCFLQARIK 1402
            |.:||:...:|||...|||:||..:.       :.|:| ||.|   :.::.|.:...||..||
Yeast  2108 LNKVHQHVVNNKMDASNLAIVFSMSF-------INQED-LANS---MGSRLGAVQTILQDFIK 2159

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGAP1A	NP_001284725.1	RhoGEF	675..857	CDD:214619	43/196 (22%)
		PH_BCR_arthropod	851..1037	CDD:270174	42/244 (17%)
		C2	1079..1187	CDD:472691	23/141 (16%)
		RhoGAP	1203..1398	CDD:470621	64/203 (32%)
BEM2	NP_011082.3	RasGEF	592..860	CDD:214539	53/305 (17%)
		REM	1120..1291	CDD:470601	26/183 (14%)
		PH	<1877..1948	CDD:459697	12/72 (17%)
		RhoGAP	1981..2134	CDD:459875	52/162 (32%)