Protein Alignment RhoGAP1A and BEM3

DIOPT Version :10

• Sequence 1: NP_001284725.1
• Gene: RhoGAP1A / 5740235
• FlyBaseID: FBgn0025836
• Length: 1404
• Species: Drosophila melanogaster
• Sequence 2: NP_015210.1
• Gene: BEM3 / 855988
• SGDID: S000006036
• Length: 1128
• Species: Saccharomyces cerevisiae
• Alignment Length: 1425
• Identity: 281/1425 - (19%)
• Similarity: 459/1425 - (32%)
• Gaps: 529/1425 - (37%)

  Fly   138 DNKYTTHTNAVIGGIGSEKVSGPLHSTQITADGKRTGELYLKREEDKCKDSNS-----------E 191
            ||..|||                        .|..|.||..:..:.:.|...|           :
Yeast     3 DNLTTTH------------------------GGSTTLELLAQYNDHRSKKDKSIEHIEKGTCSGK 43

  Fly   192 DTDGALQQCITE-LAASLKAQPDNVAGGRLENDVDIDKPLRKSTSQYVTVIQVKEAKDKESADGT 255
            :.:.:..:..|| :...|:.|........||..:|:   |:|:....:.|  |.|....:|.|..
Yeast    44 ERNPSYDEIFTENIKLKLQVQEYETEIESLEKVIDM---LQKNREASLEV--VLEQVQNDSRDSY 103

  Fly   256 TSSSGIGEPQQQPTPPSTFSRYSEATA-----PPLSPSLTSYAAHVEAKKKMPPRPP-------- 307
            .:.      |....||.:..|.:...:     |.|||.| ...:.|..:..:.|..|
Yeast   104 VND------QSFVLPPRSAERKAHIKSLNLPIPTLSPPL-QQGSDVALETSVTPTVPQIGVTSNT 161

  Fly   308 ------------------------PKNIRR-VEPPT-IPSQQLSSSPKRETYMIN--TMPSSSSG 344
                                    ||.|.| |..|| |.|:||:|.....||..:  |:.|.:.|
Yeast   162 SISRKHLQNMILNDEIEANSSFSSPKIINRSVSSPTKIHSEQLASPAASVTYTTSRITIKSPNKG 226

  Fly   345 SLCIDSLGNSLERNIKPSDI-----------LRQKSSDNLDSKYSAKRKTTPELSKFVNVNNPEL 398
            |.  ..|...|.....|:.:           |:..:|:|||                      ||
Yeast   227 SK--SPLQERLRSPQNPNRMTAVINNHLHSPLKASTSNNLD----------------------EL 267

  Fly   399 MEELGRKMVSKADSLEQTRRNDSSPSGCGSLGRTSSTPGRSLTPGRTGVGASPTGSLS-KTGKLA 462
            .|...:::.:.|     .::||...|...|...|:.||        |....||:..|. |.|:..
Yeast   268 TESKSQQLTNDA-----IQKNDRVYSSITSSAYTTGTP--------TSAAKSPSSLLEVKEGENK 319

  Fly   463 MADSSSTSSLERRSCLQAVDADIGTESRESGANQRSVGSKESLTSQGISEVQIKSYESVSSLSSE 527
            ....|..|..:.....|.:|:..|.|      :..:..||:.|              |:.|..:|
Yeast   320 ALGFSPASKEKLDDFTQLLDSSFGEE------DLVNTDSKDPL--------------SIKSTINE 364

  Fly   528 SAKAVASQQSAAAGGALAECEPYYDSVPLDNGDGEYVFIKPGRTGSSSSRDDLSTPGSSILPLPN 592
            |.                   |...:.|        .|..|..:|:..:    |||.||.|..|.
Yeast   365 SL-------------------PPPPAPP--------TFFSPTSSGNIKN----STPLSSHLASPV 398

  Fly   593 VADP-------------ESPGRTSNYVNIDFFLQQGIETRSSSLDSDGEYEGPPI---------- 634
            :.:.             :.|..||:..|:...|  ...::::||..:     ||:
Yeast   399 ILNKKDDNFGAQSAKNLKKPVLTSSLPNLSTKL--STTSQNASLPPN-----PPVESSSKQKQLG 456

  Fly   635 ----------LRTISHDDQTTTQTTPGAIRKNLVSAILVCGNARGAKIRSILSSI-------IQS 682
                      |.|.|...|.:.:|    :|:...|::        :.::|:..|:       :|.
Yeast   457 ETASIHSTNTLNTFSSTPQGSLKT----LRRPHASSV--------STVKSVAQSLKSDIPLFVQP 509

  Fly   683 E---TIYVECLN-----------------------KMMQYKKAIHATLTTSQPVIREEEENTIFF 721
            |   ||.:|.|:                       :|.::.|:||
Yeast   510 EDFGTIQIEVLSTLYRDNEDDLSILIAIIDRKSGKEMFKFSKSIH-------------------- 554

  Fly   722 KIDELYDLHTGFLSDLKTIVSHEGGDVLIGEPFRRLAEMF-----DLYSAFLHNYKNAIETVKKC 781
            |:.|| |::         :.||.....|...|.|:|.:..     |.....|:.|..:|.:|
Yeast   555 KVREL-DVY---------MKSHVPDLPLPTLPDRQLFQTLSPTKVDTRKNILNQYYTSIFSV--- 606

  Fly   782 SANNPQFKKIVS-TIVLNLQTEQSLT---LED-------LLHKP--------------VARVQIN 821
                |:|.|.|. .|...:.|:..:|   ::|       ||.:|              :.|..:.
Yeast   607 ----PEFPKNVGLKIAQFISTDTVMTPPMMDDNVKDGSLLLRRPKTLTGNSTWRVRYGILRDDVL 667

  Fly   822 ALVFNDLLRETPNAHPDHQPLRQAQKIIQMFLNQFNVVNQRLPIESNRNLRRMVRNSFIVELVDG 886
            .|...:.|.||         ::..|..|::..|        ||.:     |...||.|   |:..
Yeast   668 QLFDKNQLTET---------IKLRQSSIELIPN--------LPED-----RFGTRNGF---LITE 707

  Fly   887 HRKLRHLFLFNDVIACAKYKALGRDRIDYELKWFIPLKDVSIYEEADPAVELKESSPANISQVKR 951
            |:|       :.:....||........:.|| |.....|.     .||:..|..||..|.:
Yeast   708 HKK-------SGLSTSTKYYICTETSKEREL-WLSAFSDY-----IDPSQSLSLSSSRNAN---- 755

  Fly   952 NLRSVRDQLAMEVANSGSGAFRSGDKYRRKLADLESQLVLATPNLVLRLGNKANNKTITFFLSSD 1016
                          ::.|.:..|...:..|..:.... ...||:.|..|..:.||.......|::
Yeast   756 --------------DTDSASHLSAGTHHSKFGNATIS-ATDTPSYVTDLTQEYNNNNNISNSSNN 805

  Fly  1017 FERTQWIDSILSLKQKCNLPGANTINSLEVTAFIVAMQKGMKTEMGSYLMRNTNDESL-----LV 1076
            ...:..|||                |....:.|:.:.....:.|..|   |.....||     |.
Yeast   806 IANSDGIDS----------------NPSSHSNFLASSSGNAEEEKDS---RRAKMRSLFPFKKLT 851

  Fly  1077 GDLYMGVHGLEGLEQANDLYICVEVDSYGHYFRKATTKKICRSQTPLWNESFMLELEGSQNVRIL 1141
            |......|  .|:..:||.......||    ..|:.:||             ::|:..|.|
Yeast   852 GPASAMNH--IGITISNDSDSPTSPDS----IIKSPSKK-------------LMEVSSSSN---- 893

  Fly  1142 LYEAKERPLLKAKHILKLSLSWLTETTQPKSIKLTETLELGCSFRFIPGELFRGSTKP---GALF 1203
                                                                 .||.|   .|:|
Yeast   894 -----------------------------------------------------SSTGPHVSTAIF 905

  Fly  1204 GAKMSQVLKREKR------DIPFIIGACIREV-ERRGMLEVGCYRVSGSAS-------------- 1247
            |:.:...|:....      |:|.::..|:..: :.||:.|.|.:|:|||::
Yeast   906 GSSLETCLRLSSHKYQNVYDLPSVVYRCLEYLYKNRGIQEEGIFRLSGSSTVIKTLQERFDKEYD 970

  Fly  1248 -DLAKLKKAFESDAYEAEQLLREVDIHSVTGILKTFLRELPEALFTDQLYPRFFDTFSAFSNNNE 1311
             ||.:..::.|:...||...| .:.:::|:|:||.:||:||..||.|:.:..|.....  .|:|.
Yeast   971 VDLCRYNESIEAKDDEASPSL-YIGVNTVSGLLKLYLRKLPHLLFGDEQFLSFKRVVD--ENHNN 1032

  Fly  1312 STRINELLKVFEE---LPQANKASITSILDHLIRVHEKETDNKMSLHNLAMVFGPTLLRP 1368
            ..:|:...|...|   :|.||.:.:.::.:.|:|::|....|||:|.||.:||.|||..|
Yeast  1033 PVQISLGFKELIESGLVPHANLSLMYALFELLVRINENSKFNKMNLRNLCIVFSPTLNIP 1092

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGAP1A	NP_001284725.1	RhoGEF	675..857	CDD:214619	45/244 (18%)
		PH_BCR_arthropod	851..1037	CDD:270174	35/185 (19%)
		C2	1079..1187	CDD:472691	12/107 (11%)
		RhoGAP	1203..1398	CDD:470621	55/191 (29%)
BEM3	NP_015210.1	PX	520..622	CDD:214610	25/138 (18%)
		PH_Bem3	633..741	CDD:270096	27/145 (19%)
		RhoGAP	904..1110	CDD:470621	55/192 (29%)