Protein Alignment MCOLN1 and Trpml

DIOPT Version :10

• Sequence 1: NP_065394.1
• Gene: MCOLN1 / 57192
• HGNCID: 13356
• Length: 580
• Species: Homo sapiens
• Sequence 2: NP_649145.1
• Gene: Trpml / 40152
• FlyBaseID: FBgn0262516
• Length: 652
• Species: Drosophila melanogaster
• Alignment Length: 632
• Identity: 234/632 - (37%)
• Similarity: 351/632 - (55%)
• Gaps: 97/632 - (15%)

Human     2 TAPAGPRGSETERLLTPNPGYGTQAGPSPAPPT------PPE----------EEDLRRRLKYFFM 50
            :.|..|        :.|.|   ..||...|||:      .||          ||.:||:|::|||
  Fly    51 STPVAP--------VVPMP---ISAGSGTAPPSVDGREEQPEFPGSSAASYQEERMRRKLQFFFM 104

Human    51 SPCDKFRAKGRKPCKLMLQVVKILVVTVQLILFGLSNQLAVTFREENTIAFRHLFLLGYSDG--- 112
            :|.:|::||.:.|.|.::|:|||.:||:||.||..|....:.:..:|..||.||||.|:...
  Fly   105 NPIEKWQAKRKFPYKFVVQIVKIFLVTMQLCLFAHSRYNHINYTGDNRFAFSHLFLRGWDSSREV 169

Human   113 -----ADDTFAAYTREQLYQAIFHAVDQYLALPDVSLGRYAYVRGGGDPWTNGS--GLALCQRYY 170
                 |...||.|.:.:.:..:.:||:.| |....|:|.|.|      |..|.:  .|.||.:.|
  Fly   170 ESYPPAVGPFALYLKSEFFDTVQYAVNGY-ANVSRSIGPYDY------PTPNNTMPPLKLCLQNY 227

Human   171 HRGHVDPANDTFDIDPMVVTDCIQVDPPERPPPPPSDDLTLL--ESSSSYKNLTLKFHKLVNVTI 233
            ..|.:...|:::..||.:...|      ||.||    ::|.:  |:....:::.:.|..||:..:
  Fly   228 REGTIFGFNESYIFDPHIDEVC------ERLPP----NVTTIGVENYLRQRDVEVNFASLVSAQL 282

Human   234 HFRLKTINLQSLINN---EIPDCYTFSVLITFDNKAHSGRIPISLETQAHIQECKHPSVFQHG-- 293
            .|::||:|.::  |.   ..|||:.|.:.|||:|:.|.|::.:||:.:|...:|       ||
  Fly   283 TFKIKTVNFKA--NGGPLSAPDCFRFDISITFNNRDHDGQMLLSLDAEATRLKC-------HGAT 338

Human   294 --------DNSFRLLFDVVVILTCSLSFLLCARSLLRGFLLQNEFVGFMWRQRGRVISLWERLEF 350
                    |:..|.:.::.|:|||:|||.||.|:|.|.:||:...|.|...|.|:.:|...||||
  Fly   339 DFISDANFDSMLRSVLNIFVLLTCALSFALCTRALWRAYLLRCTTVNFFRSQFGKELSFDGRLEF 403

Human   351 VNGWYILLVTSDVLTISGTIMKIGIEAKNLA--SYDVCSILLGTSTLLVWVGVIRYLTFFHNYNI 413
            ||.|||:::.:|||.|.|:.:|..||.:.|.  .:|.||:.||...||||.||:|||.||..||:
  Fly   404 VNFWYIMIIFNDVLLIIGSALKEQIEGRYLVVDQWDTCSLFLGIGNLLVWFGVLRYLGFFKTYNV 468

Human   414 LIATLRVALPSVMRFCCCVAVIYLGYCFCGWIVLGPYHVKFRSLSMVSECLFSLINGDDMFVTFA 478
            :|.||:.|.|.::||.....:||.|:.||||::|||||:|||||:..|||||:||||||||.|||
  Fly   469 VILTLKKAAPKILRFLIAALLIYAGFVFCGWLILGPYHMKFRSLATTSECLFALINGDDMFATFA 533

Human   479 AMQAQQGRSSLVWLFSQLYLYSFISLFIYMVLSLFIALITGAYDTIKHPGGAGAEESELQAYI-A 542
            .:.:   :::.:|.|.|:||||||||:||:|||||||:|..||||||.....|...::|:|:: .
  Fly   534 TLSS---KATWLWWFCQIYLYSFISLYIYVVLSLFIAVIMDAYDTIKAYYKDGFPTTDLKAFVGT 595

Human   543 QCQDSPTSGKF--------RRGSGSACSLLCCCGR-----DPSEEHS 576
            :..:..:||.|        :.........:|||||     :|::.:|
  Fly   596 RTAEDISSGVFMTDLDDFDQTSFLDVVKSICCCGRCGRHQEPAQPNS 642

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
MCOLN1	NP_065394.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..38		11/51 (22%)
		Dileucine motif, mediates targeting to lysosomes. /evidence=ECO:0000269|PubMed:16497227	11..16		0/4 (0%)
		Interaction with phosphoinositides. /evidence=ECO:0000269|PubMed:22733759	42..62		10/19 (53%)
		Extracellular/lumenal pore loop. /evidence=ECO:0000305|PubMed:28112729	107..121		4/21 (19%)
		ELD_TRPML1	112..292	CDD:410966	51/194 (26%)
		PKD_channel	<388..524	CDD:462341	79/135 (59%)
		Selectivity filter. /evidence=ECO:0000305|PubMed:29019983	469..474		4/4 (100%)
		Required for palmitoylation and association with membranes. /evidence=ECO:0000269|PubMed:16497227	565..567		1/1 (100%)
		Dileucine internalization motif, mediates AP2 complex-dependent internalization. /evidence=ECO:0000269|PubMed:16497227	573..578		1/4 (25%)
Trpml	NP_649145.1	ELD_TRPML	174..335	CDD:410965	51/186 (27%)
		Ion_trans	<403..578	CDD:459842	99/177 (56%)