Protein Alignment Foxo1 and CHES-1-like

DIOPT Version :10

• Sequence 1: NP_062713.2
• Gene: Foxo1 / 56458
• MGIID: 1890077
• Length: 652
• Species: Mus musculus
• Sequence 2: NP_511071.3
• Gene: CHES-1-like / 31678
• FlyBaseID: FBgn0029504
• Length: 1268
• Species: Drosophila melanogaster
• Alignment Length: 799
• Identity: 162/799 - (20%)
• Similarity: 265/799 - (33%)
• Gaps: 283/799 - (35%)

Mouse    26 PLPRPE-----FNQSNSTTSSPAPSGGAA-ANPDAAASL---ASASAVSTDFMSNLSLLEESEDF 81
            |:|:..     |...|..||:.:.|..:: :.|::.|.|   .|.:.|:|..:.:...|.....|
  Fly   460 PVPKTSSFSTVFEAVNYATSAASNSSSSSNSTPNSQAILHGQESPAVVTTTTLISAGTLPPGYSF 524

Mouse    82 ----ARAPGCVAVAAAAAASRGLCGDFQGPEAGCVHPAPPQPPPTGPL----------------- 125
                |..|..::..|                    |.|.|:.||...|
  Fly   525 VNQVASTPHVLSTPA--------------------HVASPETPPGAALLPGTYYATSTTSAGTTT 569

Mouse   126 ------------SQPPP-VPPSAAAAAGPLAG--------------------------------- 144
                        |.||| :.|::....|.|.|
  Fly   570 TTLQARSLQLSVSPPPPNMTPTSQVHNGSLGGGGGGGGGSGGGGGGGGANLRSPNSYASYDNEDS 634

Mouse   145 ---------------QPR-KTSSSRRNAWGN---------------------------------- 159
                           .|: ..|.:..|.:||
  Fly   635 LKEFDLVVSSRLHTSTPQYSVSKNGANGYGNGNSNVNGSGAGSNTPQKQKHPNNVPYDPLVHTNN 699

Mouse   160 ---LSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFI 221
               .|::.||..|||.|.||.|.:.:||.|:|:..||||     .:..|||||:||||||:..|:
  Fly   700 KPPYSFSSLIFMAIEGSNEKALPVKEIYAWIVQHFPYFK-----TAPNGWKNSVRHNLSLNKSFV 759

Mouse   222 RVQN-EGTGKSSWWMLNPEGGKS-----GKSPRRRAASMDNNSKFAKS-RGRAAKKKASLQSGQE 279
            :|:. ...||.|.|.:.|:..::     .:||....:::|..|...|| .|.:|..  ||..|..
  Fly   760 KVEKAPNMGKGSLWRVEPQQRQNLIQALNRSPFFPNSAVDKISPSLKSPSGGSAYD--SLDGGGS 822

Mouse   280 G------------------------PGDSPGSQFSKW---------PASP-----GSHSNDD--- 303
            |                        |..|.|...:..         |.||     |||:..|
  Fly   823 GSVSSAQPVAGGAGVPAAAAVALSTPTKSNGLALANGASQATNAARPHSPNGGGSGSHARFDPYL 887

Mouse   304 FDNWS-TFRPRTSSNASTISGRLSPIMTEQDDLGD---GDVHSLVYPPSAAKM------------ 352
            |.|.| .||   :....|:.   .|::  ||.|.|   ||.|:.....|.:|.
  Fly   888 FPNLSKAFR---NIREDTVG---QPML--QDALDDELSGDYHNNNNNNSGSKYNYMGANGSGGAG 944

Mouse   353 ----------ASTLPSLSEISNPENMENLLD------NLNLLSSPTSLTVSTQSSPGSMMQQTPC 401
                      ||...:.:.::.....:::.|      .|.|...|...:...|:..|.::..:|.
  Fly   945 SGGVGSNANGASDGINFARLARDCGADSIDDVHAAAAMLYLKHGPKIYSEPFQNGSGPVITSSPS 1009

Mouse   402 --YSFAPPNTSLNSPSPNYSKYTYGQSSMSPLPQMPMQTLQDSKSSYGGL-NQYNCAPGLLKELL 463
              ::::....| |:.|.:.:..|.|.:..|     ..|.:...:::.||. :..|||    ....
  Fly  1010 EDHTYSAGGNS-NADSGSSTPLTNGNALAS-----VAQAVAQGQNNQGGAGSDSNCA----SSDA 1064

Mouse   464 TSDSPPHNDIMSPVDPGVAQPNSRVLGQNVMMGPNSVMPAYGSQASHNKMMNPSSHTHPGHAQQT 528
            ..||...|..::|.:....|.:...:...:.:..:|::.. ||.|:.:...|.||.:..|...:.
  Fly  1065 AYDSSEENHNITPEEMADRQRHRDGVDALLSLSGSSIVEC-GSVATTHYHSNGSSGSSHGSPHKR 1128

Mouse   529 ASVNGRTLPHVVNTMPHTSAMNRLTPVKTPLQVPLSHPMQMSALGSYSSVSSCNGYG-RMGVL-- 590
            ||.:.....|:        ..:|...::...|....|..|   .||..:|.: ||.| :|.:|
  Fly  1129 ASSHSLEEEHL--------QQHREQQLQQQQQQQQHHHQQ---FGSSQAVYT-NGSGSKMALLSS 1181

Mouse   591 ----------HQEKLPSDL 599
                      .|:::..|:
  Fly  1182 AAANVALAQQQQQQIHQDM 1200

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Foxo1	NP_062713.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..62		11/44 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	112..154		16/120 (13%)
		FH_FOXO1	148..246	CDD:410834	40/140 (29%)
		DNA-binding. /evidence=ECO:0000250|UniProtKB:Q12778	208..215		5/6 (83%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	231..342		38/161 (24%)
		DNA-binding. /evidence=ECO:0000250|UniProtKB:Q12778	231..234		1/2 (50%)
		Nuclear localization signal. /evidence=ECO:0000250	248..250		0/1 (0%)
		Sufficient for interaction with NLK. /evidence=ECO:0000269|PubMed:20061393	280..562		66/357 (18%)
		Required for interaction with RUNX2. /evidence=ECO:0000269|PubMed:21471200	360..456		18/104 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	383..410		3/28 (11%)
		FOXO_KIX_bdg	420..501	CDD:465228	15/81 (19%)
		Required for interaction with SIRT1. /evidence=ECO:0000269|PubMed:15220471	459..463		0/3 (0%)
		FOXO-TAD	594..630	CDD:435506	1/6 (17%)
CHES-1-like	NP_511071.3	FH_FOXN2-like	700..775	CDD:410805	35/79 (44%)