Protein Alignment CDK5RAP2 and cnn

DIOPT Version :10

• Sequence 1: NP_060719.4
• Gene: CDK5RAP2 / 55755
• HGNCID: 18672
• Length: 1893
• Species: Homo sapiens
• Sequence 2: NP_001246293.1
• Gene: cnn / 36491
• FlyBaseID: FBgn0013765
• Length: 1378
• Species: Drosophila melanogaster
• Alignment Length: 1995
• Identity: 379/1995 - (18%)
• Similarity: 676/1995 - (33%)
• Gaps: 808/1995 - (40%)

Human     9 DVTVPGTL-------SGCSGLVPSVPDDLDGINPNAGLGNGLLPNVSEETVSPTRARNMKDFENQ 66
            |.|.||.|       |..|..||..|          |.||..|         |::.|::::.|.|
  Fly    61 DDTSPGALQDVTMENSYASFDVPRPP----------GGGNSPL---------PSQGRSVRELEEQ 106

Human    67 ITELKKENFNLKLRIYFLEE-RMQQEFHGPTEHIYKTNIELKVEVESLKRELQEREQLLIKASKA 130
            ::.|:||||||||||||||| :........||.:.|..|:.|:|:.:|::.:..:.:||..|::|
  Fly   107 MSALRKENFNLKLRIYFLEEGQPGARADSSTESLSKQLIDAKIEIATLRKTVDVKMELLKDAARA 171

Human   131 V---ESLAEAGGSEIQRVKEDARKKV----------QQVEDLL-TKRILLLEKDVTAAQAEL--- 178
            :   |.|......:.|.:.::.::::          |.||::. |:::|.||.:|...:.||
  Fly   172 ISHHEELQRKADIDSQAIIDELQEQIHAYQMAESGGQPVENIAKTRKMLRLESEVQRLEEELVNI 236

Human   179 --EKAFAGTETEKALRLRLESKLSEMKKMHEGDLAMALVLDEKDRLIEELKLSLKSKEALIQCLK 241
              ....|..|.|..|..||||..:...|:.|                    |::|:.| |::.|:
  Fly   237 EARNVAARNELEFMLAERLESLTACEGKIQE--------------------LAIKNSE-LVERLE 280

Human   242 EEKSQMACPDENVSSGELRGLCAAPREEKERETEAAQMEHQKER---------NSFE---ERIQA 294
            :|.:.....:|.:.|.::. |.|..:|.::..|....::|..:|         |:.:   :.|..
  Fly   281 KETASAESSNEAIDSLKVE-LEACRKENQDLVTSIRTLKHDMKRQVRSMKEAANTMDVQRQSILL 344

Human   295 LEEDLREKEREIATEKKNSLKRDKAIQGLTMALKSKEKKVEELNSEIEKL---SAAFAKAREALQ 356
            ||..::.||:...:.:||.|              :.|..:.:||:|:|.:   :..|.:..|.||
  Fly   345 LEATIKRKEKSCGSMQKNVL--------------NYEALIAKLNAELETMRQQNVYFRELSENLQ 395

Human   357 KAQTQEF-QGSEDYE----TALSGK-----------EALSAALRSQNLTKSTENHRLRRS----- 400
            :.:.::. :|....:    ||.:|:           |.|....:|...|.:.....:|.|
  Fly   396 QKEVRQLDRGVAIVQPMRMTADAGRFVWQSGTIVAQEPLPLERQSAAATSNVSVSAVRLSSGPPP 460

Human   401 --------------------------IKKITQELSDLQQE----RERLEKDLEEAHREKSKGDCT 435
                                      |..:...|..|..:    ..||...|:..|       ..
  Fly   461 DQTYPVNGHDRAKYGLLQLFAAKLDWISAVPLALGHLALKIILLAMRLHSCLQNVH-------IP 518

Human   436 IRDLRNEVEKLRNEVNEREKAMENRYKSLLSESNKKLHNQEQVIKHLTESTNQKDVLLQKFNEKD 500
            :|..|:...:|.:::.|            |.|:.:||..:|::                  :|:.
  Fly   519 LRANRDLGAQLADKICE------------LQEAQEKLKERERI------------------HEQA 553

Human   501 LEVIQQNCYLMAAEDLELRSEGLITEKCSSQQPPGSKTIFSKEKKQSSDYEELIQVLKKEQDIYT 565
            ...||:                 :.:|.|||:....|  .::|.:||::         ||.|...
  Fly   554 CRTIQK-----------------LMQKLSSQEKEIKK--LNQENEQSAN---------KENDCAK 590

Human   566 HLVKSLQESDSINNLQAELNKIFALRKQLEQDVLSYQNLRKTLEEQISEIRRREEESFSLYSDQT 630
            .::.......|:::.:|...::..             |||...|.:|:|   :||:...|
  Fly   591 TVISPSSSGRSMSDNEASSQEMST-------------NLRVRYELKINE---QEEKIKQL----- 634

Human   631 SYLSICLEENNRFQVEHFSQEELKKKVSDLIQLV-KELYTDNQHLKKTIFDLSCMGFQGNGFPDR 694
                               |.|:|||.::|..|| |||:..|:.:::.
  Fly   635 -------------------QTEVKKKTANLQNLVNKELWEKNREVERL----------------- 663

Human   695 LASTEQTELLASKEDEDTIKIGEDDEINFLSDQHLQQS---NEIMKDLSKGGCKNGYLRHTESKI 756
                  |:|||::: :...:|.|:.    ..:..||||   .|.|:.|.:.
  Fly   664 ------TKLLANQQ-KTLPQISEES----AGEADLQQSFTEAEYMRALERN-------------- 703

Human   757 SDCDGAHAPGCLEEGAFINLLAPLFNEKATLLLESRPDLLKVVRELLLGQLFLTEQEVSGEHLDG 821
                                  .|...|..:|.:...|  ......::|||.|..|:       .
  Fly   704 ----------------------KLLQRKVDVLFQRLAD--DQQNSAVIGQLRLELQQ-------A 737

Human   822 KTEKTPKQKGELVHFVQTNSFSKPHDELKLSCEAQLVKAGEVPKVGLKDASVQTVATEGDLLRFK 886
            :||            |:|      .|:.:|.|              :...||.|...| :|..|
  Fly   738 RTE------------VET------ADKWRLEC--------------VDVCSVLTNRLE-ELAGF- 768

Human   887 HEATREAWEEKPINTALSAEHRPENLHGVPGWQAALLSLPGITNREAKKSRLPILIKPSRSLGNM 951
                        :|:.|  :|:.            :|.:.....|.|.:..:...:..|:||...
  Fly   769 ------------LNSLL--KHKD------------VLGVLAADRRNAMRKAVDRSLDLSKSLNMT 807

Human   952 YRLPAT---QEVVTQL--QSQILELQGELKEFKTCN--KQLHQKLILAEAVMEGRPTPDKTLLNA 1009
            ..:.||   .:.:.||  .|:||..:|:... ||.|  ::||....:|..|
  Fly   808 LNITATSLADQSLAQLCNLSEILYTEGDASH-KTFNSHEELHAATSMAPTV-------------- 857

Human  1010 QPPVGAAYQDSPGEQKGIKTTSSVWRDKEMDSDQQRSYEIDSEICPPDDLASLPSCKENPEDVLS 1074
                    ::...|.|.:|......|..|....::||..:.|:        ...:..|:
  Fly   858 --------ENLKAENKALKKELEKRRSSEGQRKERRSLPLPSQ--------QFDNQSES------ 900

Human  1075 PTSVATYLSSKSQPSAKVSV--MGTDQSESINTSNETEYLKQKIHDLETELEGYQNFIFQLQKHS 1137
                    .:.|:|..|||:  :|.|:     |||.....:|.|.  |:|.||.
  Fly   901 --------EAWSEPDRKVSLARIGLDE-----TSNSLAAPEQAIS--ESESEGR----------- 939

Human  1138 QCSEAIITVLCGTEGAQDGLSKPKNGSDGEEMTFSSLHQVRYVKHVKILGPLAPEMIDSRVLENL 1202
                     .|.|.  ||   :.:|                                 |..:..|
  Fly   940 ---------TCATR--QD---RNRN---------------------------------SERIAQL 957

Human  1203 KQQLEEQEYKLQKEQNLNMQLFSEIHNLQNKFRDLSPPRYDSLVQSQARELSLQRQQIKDGHGIC 1267
            ::|:.:::     |:.||:|  .::..|.|:::
  Fly   958 EEQIAQKD-----ERMLNVQ--CQMVELDNRYK-------------------------------- 983

Human  1268 VISRQHMNTMIKAFEELLQASDVDYCVAEGFQEQLNQCAELLEKLEKLFLNGKSVGVEMNTQNEL 1332
                                           |||| :|.::.::||:|....:::..::
  Fly   984 -------------------------------QEQL-RCLDITQQLEQLRAINEALTADL------ 1010

Human  1333 MERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNETEKTSVMVNSFSQDLLME 1397
                                    ||:..:|
  Fly  1011 ------------------------HAIGSHE---------------------------------- 1017

Human  1398 HIQEIRTLRKRLEESIKTNEKLRKQLERQGSEFVQGSTSIFASGSELHSSLTSEIHFLRKQNQAL 1462
                    .:|:.|       |::|||.:..:..|...:        ||:||::......:.|||
  Fly  1018 --------EERMVE-------LQRQLELKNQQIDQLKLA--------HSTLTADSQITEMELQAL 1059

Human  1463 NAMLIKGSRDKQKENDKLRESLSRKTVSLEHLQREYASVK-------EENERLQKEGSEK----- 1515
                    :.:.:|.::|...      |:|.||.:...:|       ||:|||.:|..|:
  Fly  1060 --------QQQMQEIEQLHAD------SVETLQSQLQKLKLDAVQQLEEHERLHREALERDWVAL 1110

Human  1516 ---ERHNQQLIQEVRCSGQELSRVQEEVKLRQQLLSQNDKLLQSLRVELKAYEKLDEEHRRLREA 1577
               :...|||::..|    .|...||..|..:|.|.:|:...::|:      ::|||...:..:|
  Fly  1111 TTYQEQAQQLLELQR----SLDYHQENEKELKQTLVENELATRALK------KQLDESTLQASKA 1165

Human  1578 SGEGWKGQDPFRDLHSLLMEIQALRLQLERSIETSSTLQSRLKEQLARGAEKAQEGALTLAVQAV 1642
            ..|..|.   :.|...|....:.||||||...|.    ..:|.::.:..::.:|.|..:   :.|
  Fly  1166 VMERTKA---YNDKLQLEKRSEELRLQLEALKEE----HQKLLQKRSNSSDVSQSGYTS---EEV 1220

Human  1643 SIPEVPLQPDKHDGDKYPMESDNSFDLFDSSQAVTPKSVSETPPLSGNDTDSLSCDSGSSATSTP 1707
            ::|..|                      .|.||.|.|..:..  :.|...::.|.|.|..:.:
  Fly  1221 AVPMGP----------------------PSGQATTCKQAAAA--VLGQRVNTSSPDLGIESDA-- 1259

Human  1708 CVSRLVTGHHLWASKNGRHVLGLIEDYEALLKQISQGQRLL---AEMDIQTQEAPSSTSQELGTK 1769
                                 |.|...|     :|..||.:   .||..:...:|.:.|:|
  Fly  1260 ---------------------GRISSVE-----VSNAQRAMLKTVEMKTEGSASPKAKSEE---- 1294

Human  1770 GPHPAPLSKFVSSVSTAKLTLEEAYRRLKLLWRVSLPEDGQCPLHCEQIGEMKAEVTKLHKKLFE 1834
              ..:|.||  |:|:|...|:.:                      |.::....||   |.:||..
  Fly  1295 --STSPDSK--SNVATGAATVHD----------------------CAKVDLENAE---LRRKLIR 1330

Human  1835 QEKKLQNTMKLLQLSKRQ----EKVIFDQLVVTHKILRKARGNLE 1875
            .::..::|.:.|:::.:.    ||.|.:|::.||.:||..|.|:|
  Fly  1331 TKRAFEDTYEKLRMANKAKAQVEKDIKNQILKTHNVLRNVRSNME 1375

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CDK5RAP2	NP_060719.4	CM1 motif, interacts with the gTuRC. /evidence=ECO:0000269|PubMed:39321809	51..94		20/43 (47%)
		Interaction with NCKAP5L. /evidence=ECO:0000269|PubMed:26485573	58..196		48/157 (31%)
		Cnn_1N	61..129	CDD:462333	28/68 (41%)
		Smc	146..>458	CDD:440809	73/393 (19%)
		Smc	<401..>621	CDD:440809	39/223 (17%)
		Interaction with MAPRE1. /evidence=ECO:0000269|PubMed:19553473	926..1208		53/290 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1015..1071		9/55 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1084..1105		7/22 (32%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1347..1381		3/33 (9%)
		Smc	<1392..>1641	CDD:440809	58/263 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1500..1521		8/35 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1646..1706		10/59 (17%)
		Interaction with PCNT and AKAP9. /evidence=ECO:0000269|PubMed:20466722	1726..1893		37/157 (24%)
		Interaction with CDK5R1. /evidence=ECO:0000250	1726..1768		11/44 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1754..1774		3/19 (16%)
		Required for centrosomal attachment, Golgi localization and CALM1 interaction	1861..1870		4/8 (50%)
cnn	NP_001246293.1	Cnn_1N	101..170	CDD:462333	28/68 (41%)
		Smc	<138..>404	CDD:440809	65/301 (22%)
		SMC_prok_B	498..1200	CDD:274008	214/1238 (17%)
		Smc	<929..>1247	CDD:440809	98/593 (17%)