Protein Alignment KIF21A and KIP1

DIOPT Version :10

• Sequence 1: NP_001365368.1
• Gene: KIF21A / 55605
• HGNCID: 19349
• Length: 1675
• Species: Homo sapiens
• Sequence 2: NP_009490.1
• Gene: KIP1 / 852216
• SGDID: S000000159
• Length: 1111
• Species: Saccharomyces cerevisiae
• Alignment Length: 1292
• Identity: 312/1292 - (24%)
• Similarity: 521/1292 - (40%)
• Gaps: 364/1292 - (28%)

Human     4 APDESSVRVAVRIRPQLAKEKIEGCHICTS----------VTPGEPQVFLGKDKAFTFDYVFDID 58
            |..:|::.|.||.|.:..:|..|...:..|          ::.|..|.:....|.:.||.||..:
Yeast    47 ATSDSNIHVYVRCRSRNKREIEEKSSVVISTLGPQGKEIILSNGSHQSYSSSKKTYQFDQVFGAE 111

Human    59 SQQEQIYIQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNI-----------VEEELG 112
            |.||.::....:..|:....|||.|:|||||||.||||||..  |:||           :.|..|
Yeast   112 SDQETVFNATAKNYIKEMLHGYNCTIFAYGQTGTGKTYTMSG--DINILGDVQSTDNLLLGEHAG 174

Human   113 IISRAVKHLFKSIEEKKHIAIKNGLPAPDFKVNAQFLELYNEEVLDLFDTTRDID--AKSKKSNI 175
            ||.|.:..|||.:....          .::.|...|||||||.:.||...:.|.|  ....|..|
Yeast   175 IIPRVLVDLFKELSSLN----------KEYSVKISFLELYNENLKDLLSDSEDDDPAVNDPKRQI 229

Human   176 RI--HEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTR 238
            ||  :.::...|...|:....:|:..|.:..|..|:|.|..|:|:.|..|||||.:|||   .|.
Yeast   230 RIFDNNNNNSSIMVKGMQEIFINSAHEGLNLLMQGSLKRKVAATKCNDLSSRSHTVFTI---TTN 291

Human   239 VCPQIDADNATDNKIISESAQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLA 303
            :..|...|:..:...:.           ..|.:.|||||||.:.|:||..:||:|...||..||.
Yeast   292 IVEQDSKDHGQNKNFVK-----------IGKLNLVDLAGSENINRSGAENKRAQEAGLINKSLLT 345

Human   304 LGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRA 368
            ||.||:||.|.|   .|:|||:|||||||||||||.::|.:||.:||:.....||.:||:||.||
Yeast   346 LGRVINALVDHS---NHIPYRESKLTRLLQDSLGGMTKTCIIATISPAKISMEETASTLEYATRA 407

Human   369 RNIKNKVMVNQDRAS--------QQINALRSE-----------ITRLQMELMEYKTGKRIIDEEG 414
            ::|||...|||..:.        |:|..||::           ||:.|::|  |::...:|||:.
Yeast   408 KSIKNTPQVNQSLSKDTCLKDYIQEIEKLRNDLKNSRNKQGIFITQDQLDL--YESNSILIDEQN 470

Human   415 VESINDMFHENAMLQTENNNLRVRIKAMQETVDALRSRITQLVSDQANHVLARAGEGNEEISNMI 479
            ::.               :|||.:||..:|      :.:.||                 :|:|::
Yeast   471 LKI---------------HNLREQIKKFKE------NYLNQL-----------------DINNLL 497

Human   480 HSYIKEIEDLRAKLLESEAVNENLRKNLTRATARAPYFSGSSTFSPTILSSDKETIEIIDLAKKD 544
            .|.       :.||:   |:.:|...:.:.      ::|.......|.|....|.|:..|.:.::
Yeast   498 QSE-------KEKLI---AIIQNFNVDFSN------FYSEIQKIHHTNLELMNEVIQQRDFSLEN 546

Human   545 LEKLKRKEKRKKKRL--QKLEESNREERSVAGKEDNTDTDQEKKEEKGVSERENNELEVEESQEV 607
            .:|.....:..:.::  |.|:..|..:.|:     |....:..:..|||:|        |.::.|
Yeast   547 SQKQYNTNQNMQLKISQQVLQTLNTLQGSL-----NNYNSKCSEVIKGVTE--------ELTRNV 598

Human   608 SDHEDEEEEEEEEEDDIDGGESSDESDSESDEKANYQA---DLANITCEIAIKQ--KLI------ 661
            :.|                             ||.:.:   .|.|||..:.:.|  :|:
Yeast   599 NTH-----------------------------KAKHDSTLKSLLNITTNLLMNQMNELVRSISTS 634

Human   662 ------DELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGS--VESYSEEKAKKVR 718
                  |...:.:|.|..:.:.:::.|..||:.|:       ..|.::||  :.|.:|     :.
Yeast   635 LEIFQSDSTSHYRKDLNEIYQSHQQFLKNLQNDIK-------SCLDSIGSSILTSINE-----IS 687

Human   719 SEYEKKLQAMNKELQRLQAAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEE--- 780
            ......|.:||..::..|:..   ::|:|.|   :.::|||:.|::..::...:..:|:.|.
Yeast   688 QNCTTNLNSMNVLIENQQSGS---SKLIKEQ---DLEIKKLKNDLINERRISNQFNQQLAEMKRY 746

Human   781 -QEKARLTES----RRNREIAQLKKDQRKRDHQLRLLEAQKRNQE--VVLRRKTEEVTALRRQVR 838
             |:....|.|    ..|:.|..||..|.|.|..:....|...|:.  ||.:..::.:.:|.....
Yeast   747 FQDHVSRTRSEFHDELNKCIDNLKDKQSKLDQDIWQKTASIFNETDIVVNKIHSDSIASLAHNAE 811

Human   839 PMSDKVAGKVTRKLSSSDAPAQDTGSSAAAVETDASRTGAQQKMRIPVARVQALP---------- 893
            .....|:       .::::...|..|.:..:..|.|            :::::||
Yeast   812 NTLKTVS-------QNNESFTNDLISLSRGMNMDIS------------SKLRSLPINEFLNKISQ 857

Human   894 ----------TPATN---GNRKKYQRKGLTGRVFISKTARMKWQLLERRVTDIIMQKMTISNMEA 945
                      |.|:|   .:.||:|                          :||...:.::|
Yeast   858 TICETCGDDNTIASNPVLTSIKKFQ--------------------------NIICSDIALTN--- 893

Human   946 DMNRLLKQREELTKRREKLSKRREKIVKENGEGDKNVANINEEMESLTANIDYINDSISDCQANI 1010
              .:::...:|:..:.|.:|          .|.:.|:..|||...||      .|..::|...||
Yeast   894 --EKIMSLIDEIQSQIETIS----------NENNINLIAINENFNSL------CNFILTDYDENI 940

Human  1011 MQMEEAKEEGETLDVTAVINACTLTEARYLL--DHFLSMGINKGL-QAAQKEAQ-IKVLE--GRL 1069
            ||:.:.::|       .:...|...::..:|  |.|.:..|.|.| :..:.||. ||.|.  ...
Yeast   941 MQISKTQDE-------VLSEHCEKLQSLKILGMDIFTAHSIEKPLHEHTRPEASVIKALPLLDYP 998

Human  1070 KQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSVPLENVEDST-----DEDAP---- 1125
            ||.:|....:|       |.|.:.:.....:..  |...::.||......|     |:..|
Yeast   999 KQFQIYRDAEN-------KSKDDTSNSRTCIPN--LSTNENFPLSQFSPKTPVPVPDQPLPKVLI 1054

Human  1126 ---LNS---------PGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSE 1170
               :||         |.:||:...|           :|..:||.||: .:|..:.:|
Yeast  1055 PKSINSAKSNRSKTLPNTEGTGRES-----------QNNLKRRFTTE-PILKGEETE 1099

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
KIF21A	NP_001365368.1	KISc_KIF4	8..372	CDD:276823	141/388 (36%)
		Cast	<364..836	CDD:431111	104/521 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	556..641		12/86 (14%)
		EnvC	640..>860	CDD:443969	49/248 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	779..804		9/32 (28%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	841..881		5/39 (13%)
		Rcc_KIF21A	936..1017	CDD:410204	17/80 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1116..1138		8/42 (19%)
		Interaction with KANK1 and KANK2. /evidence=ECO:0000269|PubMed:24120883, ECO:0000269|PubMed:29183992	1146..1167		6/20 (30%)
		WD40	1340..1653	CDD:238121
		WD 1	1346..1383
		WD 2	1386..1424
		WD40 repeat	1391..1450	CDD:293791
		WD 3	1450..1488
		WD40 repeat	1456..1491	CDD:293791
		WD 4	1491..1533
		WD40 repeat	1496..1540	CDD:293791
		WD 5	1542..1579
		WD40 repeat	1548..1581	CDD:293791
		WD 6	1583..1622
		WD40 repeat	1588..1624	CDD:293791
		WD 7	1625..1662
KIP1	NP_009490.1	KISc_BimC_Eg5	50..419	CDD:276815	146/397 (37%)
		235kDa-fam	<423..>948	CDD:130673	126/706 (18%)
		AAA_13	<562..>741	CDD:463796	44/238 (18%)