Protein Alignment PI4K2A and Pi4KIIalpha

DIOPT Version :10

• Sequence 1: NP_060895.1
• Gene: PI4K2A / 55361
• HGNCID: 30031
• Length: 479
• Species: Homo sapiens
• Sequence 2: NP_730944.2
• Gene: Pi4KIIalpha / 40670
• FlyBaseID: FBgn0037339
• Length: 710
• Species: Drosophila melanogaster
• Alignment Length: 506
• Identity: 260/506 - (51%)
• Similarity: 316/506 - (62%)
• Gaps: 102/506 - (20%)

Human    55 DRERQPLLDRARGAAAQGQTQTVAAQAQALAAQAAAAAHAAQAHRE--RNEFPEDPEFEAVVRQA 117
            :||.||||  ..|.|:.|          :..::..........|.|  .|.|.:||.|..:|.:|
  Fly   224 NRESQPLL--GGGNASHG----------SRISELLGGLGRGHDHVEFVSNTFGDDPLFSEIVAKA 276

Human   118 ELAIERCIFPERIYQGSSGSYFVKDPQGRIIAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRD 182
            |.|||..:.||||||||||||||||...:.:||||||:|||||.||||||||:.||||||||||.
  Fly   277 EGAIENGVLPERIYQGSSGSYFVKDASHQCLAVFKPKDEEPYGRLNPKWTKWMHKLCCPCCFGRA 341

Human   183 CLVLNQGYLSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQR 247
            ||:.|||||||:||||||:||.||:||:|:||.|.:|:|||:.|||.|::.|:...|..|..  .
  Fly   342 CLIPNQGYLSESGASLVDRKLNLNVVPKTRVVRLVAESFNYARIDRQKAKLKKRIKEHYPSA--H 404

Human   248 FNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAEPLPENTNRQLLLQFERLVVLDYIIRNTDRGND 312
            |||:.||.|.|||||||||||||||||||||:||||.:..:...|||||||||||||||||||||
  Fly   405 FNRMSLPLKTGSFQLFVEGYKDADYWLRRFESEPLPPSVAKSFQLQFERLVVLDYIIRNTDRGND 469

Human   313 NWLIKYDCP-------------------------------------------------------- 321
            ||||||..|
  Fly   470 NWLIKYVAPKITTQVGGTGGRTMHGVVTPKLPNALGASKIVDLNEPPQASPKPTTGGRPSNHLAG 534

Human   322 -------MDSSS--------------SRDTDWVVVKEPVIKVAAIDNGLAFPLKHPDSWRAYPFY 365
                   .||||              :.:.||.||....|::||||||||||.||||||||||::
  Fly   535 GAATSAQQDSSSLASSEDEPSSRSPPTSEPDWNVVNSAFIRIAAIDNGLAFPFKHPDSWRAYPYH 599

Human   366 WAWLPQAKVPFSQEIKDLILPKISDPNFVKDLEEDLYELFKKDPGFDRGQFHKQIAVMRGQILNL 430
            ||||||||:|||:|||:.:||::||.|||:::..:|..||::|.|||:..|.:|::|||||||||
  Fly   600 WAWLPQAKIPFSEEIKEQVLPQLSDMNFVEEICTELLYLFQQDRGFDKRLFERQMSVMRGQILNL 664

Human   431 TQALKDNKSPLHLVQMPPVIVETARSHQRSSSE---SYTQSFQSRKPFFSW 478
            ||||:|.|||:.|||||.||||      |||..   |:||.||::.|||||
  Fly   665 TQALRDGKSPVQLVQMPAVIVE------RSSGSRFFSFTQRFQNKSPFFSW 709

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PI4K2A	NP_060895.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..74		9/18 (50%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	130..136		5/5 (100%)
		PI3_PI4_kinase	133..429	CDD:395364	199/372 (53%)
		Important for substrate binding. /evidence=ECO:0000305|PubMed:24675427	157..159		1/1 (100%)
		Important for interaction with membranes. /evidence=ECO:0000305|PubMed:24675427, ECO:0000305|PubMed:25168678	165..178		10/12 (83%)
		Important for interaction with membranes. /evidence=ECO:0000305|PubMed:24675427	268..276		7/7 (100%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	305..313		7/7 (100%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	344..364		18/19 (95%)
		Important for interaction with membranes. /evidence=ECO:0000305|PubMed:24675427	359..368		6/8 (75%)
Pi4KIIalpha	NP_730944.2	Protein Kinases, catalytic domain	292..>474	CDD:473864	130/183 (71%)