Protein Alignment PPP3CA and PpN58A

DIOPT Version :10

• Sequence 1: NP_000935.1
• Gene: PPP3CA / 5530
• HGNCID: 9314
• Length: 521
• Species: Homo sapiens
• Sequence 2: NP_477384.1
• Gene: PpN58A / 37493
• FlyBaseID: FBgn0025573
• Length: 324
• Species: Drosophila melanogaster
• Alignment Length: 1316
• Identity: 239/1316 - (18%)
• Similarity: 403/1316 - (30%)
• Gaps: 490/1316 - (37%)

Human   706 SFSEDTKSPLSVPRSKSEMNYIDGEKVVKRSATLPLPARSSSLKSSPERNDWEPPDKKVDTRKYR 770
            |...::|:.:....|.:::||.:      ..:||....|:.::.:.|..|  |.|:.|: .|:.|
  Fly   322 SLGGNSKTIMIAAISPADVNYSE------TLSTLRYANRAKNIINKPTIN--EDPNVKL-IRELR 377

Human   771 AEPKSIYEYQPGKSSVLTNE-----KMSRDISPEEIDLK--NEPWYKFFSELEFGRPPPKKIWDY 828
            .|   ||:.:...||....|     |:..|:..:|...|  .|.|.:.:.|.:
  Fly   378 DE---IYKLKLMLSSDSGAELEPQLKVLEDLHKKEAQEKVLTEEWTEKWREAQ------------ 427

Human   829 TPGDCSILPRGDRKTNLEKDLNLCQAELEA-----------------DLEKVETVNKSPTAHVPQ 876
                 ||| |..|...|.|.......:.|.                 .|::.||...:..|..||
  Fly   428 -----SIL-REQRSLGLRKSGVGVVLDSEVPHLIGIHDDISTGVTLYSLKEGETTIGTDEAPYPQ 486

Human   877 ---------------------SSAVSPTPDITS-------EPPGYIYSSDFHAVKRES---DGTP 910
                                 ::.:.|.|:.|.       |.|..|...|...:.|.:   ...|
  Fly   487 DISLNGIGIAPEHCRIVLSNGNATIYPNPNATCLLNASIIEVPTAISQGDILLLGRTNMFRYNNP 551

Human   911 GGLASL--ENERQIYKSVLEGGDIPLQGLSGLKRPSSSASTKVDRKGGNAHMISSSSV------- 966
            ...|.:  |:..|...|.|   |:....|....|.:..||...|..||    :|.||:
  Fly   552 AEAAKMRQESSHQQRASRL---DLSRLSLIAASRENLCASFMSDDDGG----VSGSSLSLFGGGG 609

Human   967 ------------------------------HSRTFHT-SNAL----------GPGCKHKKPLSAA 990
                                          |.:...| .|||          ....|||     .
  Fly   610 GGAGGMASPQGAFRFRQYTPSREDAELQDEHRKILQTIENALRQLNLERTRMHEQFKHK-----I 669

Human   991 KACISEI--LPSKFKPRLSAPSA------------------------LLQEQKSVLLTS------ 1023
            :.|..|:  |.|..:.||:....                        :|..|.|.|.|.
  Fly   670 RLCTEELERLESTKRDRLTVLDCRIGELMARKEMVLWEKSNEKTQVDILVRQLSALQTQLDTKKR 734

Human  1024 ----------EKAQSCENLCVSLNDSKRGLP---------LRVGGSIENL-------LMRSRRDY 1062
                      ::.|.|..|..:.::..|..|         |::..|::|.       .:|..||.
  Fly   735 DFYEYVAKELQELQDCGRLDEASSELIRNAPQTEDYSEILLQISRSLDNYSQQYIKEFVRRNRDE 799

Human  1063 DSKSSSTMS--LQEYSTSSRRPCPLSRKAGLHFSMFYRDMHQINR------------------AG 1107
            .:|..:.:|  .|:.:.|:::...|.:|        ..::.|.||                  .|
  Fly   800 INKYEAELSEKEQQLAESTQKIADLDQK--------IMELEQQNRELLQQRTQQELELIQQKKLG 856

Human  1108 LSLGSISSSSVRDLASHFERSSLSLARGELGASQEGSEHIPKHTVSSRITAFEQLIQRSRSMPSL 1172
            ::|....::...|.....|::.|.|.....|..:.....   ||.:|..:....|     :.|:|
  Fly   857 MTLNLTHTNGADDGEGTGEQAGLLLGTNRAGTLETCDTF---HTANSDCSFVSAL-----NTPAL 913

Human  1173 DFSGRLSKSPTPVLSRSGLTSARSAESLLESTKLRPREMDGMDSGGVYSSPTCSNMADHALSFRS 1237
            :...:|  .|||..:..|..|         .|..|..| :.:.:||:..    ..|:|..:||.:
  Fly   914 NSLNQL--IPTPSSTEDGTES---------DTGDRDYE-EAISNGGLLH----GGMSDSGVSFET 962

Human  1238 LAPSEPLSICSDELDHCSNISNDSREGSGGS-------VHGDFPKHRLNKCKGTCP-ASY----- 1289
            ...::..:....|.|...:..:..|:.||.:       ...|  .|.|.....:.| ..|
  Fly   963 NRATDTAAALHTEADENESEGSVDRQSSGAASGTAKLLFQSD--DHCLLLATSSTPNPKYGGKKL 1025

Human  1290 -TRFTTI---------RKHEQQSSRQPDWR---SDSRGDRNSLLRNIYLMSPLPFRLKKPLQQHP 1341
             |..||:         :.::||..:.|..|   |||..:|.::.....:.:|...|||..:....
  Fly  1026 PTTTTTVIVTDGGGLLKSNQQQPQQSPRMRHKSSDSEEERTTITTMSSIGTPGSNRLKLSVPNRN 1090

Human  1342 RQPPHDDSSGCPAGQKADLPCQGPQDQPHSAGKPMVPTRLSSRHTMARLSHNSEPPLDRSAGLED 1406
            |...::..|.....:..|:        .|.    :.|.|:||..:....||..|
  Fly  1091 RSNSNNSKSSSMRTEIFDI--------RHG----IHPLRVSSVSSGTDGSHEEE----------- 1132

Human  1407 CTRAVNNGNPVPYSDHALDRNNNPQSELAAAHGDSESPRHFIPADYLESTEEFIRRRH----DDK 1467
                                    :.|...|||                     ||||    ||:
  Fly  1133 ------------------------EYEEEDAHG---------------------RRRHRQEVDDE 1152

Human  1468 EK--------------------LLADQRRLKRE--QEEADIAARRHTGVIPTH-HQFITNERFGD 1509
            |:                    :|.:...|:..  .::|:|..     ::.|: .:.:.:.:.|:
  Fly  1153 EERRSRGSSIDNSSITSSTANLILCEMNHLRESIASKKAEIMK-----ILETNGDKKLLDGKIGE 1212

Human  1510 LLNIDDTAKRKSGLEMRPARAKFDFKAQTLKELPLQKGDIVYIYKQIDQ-----NWYEGEHHGRV 1569
            |   .|..||...||:         |.|.:::..|..|::     |:|.     ..:......||
  Fly  1213 L---QDLQKRIVQLEI---------KIQDVEKSCLSDGEL-----QLDSMRDIPESFTDSDDSRV 1260

Human  1570 G---IFPRTYIELLPPAEKAQPRKLAPVQVLEYGEAIAKFNFNGDTQVEMSFRKGERITLLRQVD 1631
            |   |:|                  ..::..:.|...|.........:|.::|....|.:...:
  Fly  1261 GNSCIYP------------------GYMRARDQGSIYAPSVTRSLPSIEGTYRSDHLINIPTYI- 1306

Human  1632 ENWYEGRIPGTSRQGIFPITYVDVLKRPLVKTPVDY---IDLP---------YSSSPSRSATVSP 1684
                   |.|..:|                 |..:|   |:||         ||.......|:
  Fly  1307 -------IRGAGKQ-----------------THYEYEVKINLPDERWTLLRRYSRFRELHLTM-- 1345

Human  1685 QCTSHSKLIMPAPSSLPHPRRALSPEMHAITSEWISLT----VGVPGRRSLAMTPPLPPLPEASV 1745
                 .||.....:::|.|||    |:.|..:|.::.|    :....||.|.:...:|..|   :
  Fly  1346 -----KKLYGDKIATIPFPRR----ELFASNTESVAKTRRRQLETYLRRLLVVCAKIPHCP---I 1398

Human  1746 YDMDRLALSARARPSL 1761
            |:       ...||.|
  Fly  1399 YE-------GEGRPGL 1407

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PPP3CA	NP_000935.1	MPP_PP2B	41..345	CDD:277361
		Catalytic. /evidence=ECO:0000305	56..340
		SAPNY motif. /evidence=ECO:0000305|PubMed:26248042	307..311
		Interaction with PxIxIF motif in substrate. /evidence=ECO:0000269|PubMed:17498738, ECO:0000269|PubMed:17502104, ECO:0000269|PubMed:22343722, ECO:0000269|PubMed:23468591, ECO:0000269|PubMed:26248042	327..336
		Calcineurin B binding. /evidence=ECO:0000269|PubMed:12218175, ECO:0000269|PubMed:12357034, ECO:0000269|PubMed:17498738, ECO:0000269|PubMed:23468591, ECO:0000269|PubMed:27974827, ECO:0000269|PubMed:8524402	341..369
		Calmodulin-binding. /evidence=ECO:0000269|PubMed:18384083, ECO:0000269|PubMed:19404396, ECO:0000269|PubMed:25144868, ECO:0000269|Ref.34	392..406
		Autoinhibitory segment. /evidence=ECO:0000250|UniProtKB:P16298	407..414
		Autoinhibitory domain. /evidence=ECO:0000269|PubMed:8524402	465..487
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	475..521
PpN58A	NP_477384.1	MPP_superfamily	23..311	CDD:472684