Protein Alignment RHOT1 and GEM1

DIOPT Version :10

• Sequence 1: XP_047292309.1
• Gene: RHOT1 / 55288
• HGNCID: 21168
• Length: 722
• Species: Homo sapiens
• Sequence 2: NP_009351.1
• Gene: GEM1 / 851249
• SGDID: S000000046
• Length: 662
• Species: Saccharomyces cerevisiae
• Alignment Length: 628
• Identity: 204/628 - (32%)
• Similarity: 317/628 - (50%)
• Gaps: 93/628 - (14%)

Human    46 VGKTSLIMSLVSEEF----PEEVPPRAEEITIPADV--TPERVP--THIVDYSEAEQSDEQLHQE 102
            |||:|||:||...||    .:.:||    |:||.|.  :|...|  |.::|.|:::..  .|..|
Yeast    16 VGKSSLIVSLTKAEFIPTIQDVLPP----ISIPRDFSSSPTYSPKNTVLIDTSDSDLI--ALDHE 74

Human   103 ISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDL---VEYSSM----- 159
            :..|:||.:||.  :..|.|.|:..|:|...   .....:|:||..||.|.   |..::|
Yeast    75 LKSADVIWLVYC--DHESYDHVSLFWLPHFR---SLGLNIPVILCKNKCDSISNVNANAMVVSEN 134

Human   160 -----------ETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMK 213
                       |..:||:.::.||:||::.|||...::::.||..|:|:.||..||:.....|:|
Yeast   135 SDDDIDTKVEDEEFIPILMEFKEIDTCIKTSAKTQFDLNQAFYLCQRAITHPISPLFDAMVGELK 199

Human   214 PACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNV---VRKHISD----- 270
            |..:.||.|||.:||.:.|..|:|.|:...|:.|||..:....|..:|::   :.||..:
Yeast   200 PLAVMALKRIFLLSDLNQDSYLDDNEILGLQKKCFNKSIDVNELNFIKDLLLDISKHDQEYINRK 264

Human   271 -GVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNH 334
             .|...|:|..|||.|:.::.:||||||||.:||.|.|.|.|.:..:.|.|.|.:|...:.||:.
Yeast   265 LYVPGKGITKDGFLVLNKIYAERGRHETTWAILRTFHYTDSLCINDKILHPRLVVPDTSSVELSP 329

Human   335 HAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIP--W-GPDVNNTVCTNERGWITYQGFLSQ 396
            ..|.||...|.|.|:|.|..|:..||..|||..|.:|  | ..:...:...|.:|.||.||:|:|
Yeast   330 KGYRFLVDIFLKFDIDNDGGLNNQELHRLFKCTPGLPKLWTSTNFPFSTVVNNKGCITLQGWLAQ 394

Human   397 WTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQ------TQRNVFRCNVI 455
            |::||:|:......||.|.|:     ||....|:.||:.:|:..:..:      ..|.||.|.||
Yeast   395 WSMTTFLNYSTTTAYLVYFGF-----QEDARLALQVTKPRKMRRRSGKLYRSNINDRKVFNCFVI 454

Human   456 GVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVY-GQEKYLLLHDISESEF------- 512
            |...||||.:|:|.|||:.  .::.....|...|:|::.:. |::.||:|.::.|.|:
Yeast   455 GKPCCGKSSLLEAFLGRSF--SEEYSPTIKPRIAVNSLELKGGKQYYLILQELGEQEYAILENKD 517

Human   513 -LTEAEIICDVVCLVYDVSNPKSFEYCARIFKQ--HFMDSRIPCLIVAAKSDLHEVKQEYSISPT 574
             |.|    |||:||.||.|:|:||.|...:..:  |..|  :|.:.||:|:||.:.:|...|.|.
Yeast   518 KLKE----CDVICLTYDSSDPESFSYLVSLLDKFTHLQD--LPLVFVASKADLDKQQQRCQIQPD 576

Human   575 DFCRKHKMPPPQAFTCNTADAPSK------DIFVKLTTMAMYP 611
            :..       .:.|..:.....|:      ::|:|:|..|:.|
Yeast   577 ELA-------DELFVNHPLHISSRWLSSLNELFIKITEAALDP 612

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RHOT1	XP_047292309.1	Miro1	46..200	CDD:206680	55/180 (31%)
		EF_assoc_2	250..334	CDD:462444	31/92 (34%)
		EF_assoc_1	372..441	CDD:462443	23/69 (33%)
		Miro2	446..611	CDD:206679	56/181 (31%)
GEM1	NP_009351.1	P-loop containing Nucleoside Triphosphate Hydrolases	6..186	CDD:476819	55/180 (31%)
		EF_assoc_2	236..329	CDD:462444	31/92 (34%)
		EF_assoc_1	369..434	CDD:462443	23/69 (33%)
		Miro2	445..624	CDD:206679	57/183 (31%)