Protein Alignment POLR2A and Polr1A

DIOPT Version :10

• Sequence 1: NP_000928.1
• Gene: POLR2A / 5430
• HGNCID: 9187
• Length: 1970
• Species: Homo sapiens
• Sequence 2: NP_523743.1
• Gene: Polr1A / 36617
• FlyBaseID: FBgn0019938
• Length: 1642
• Species: Drosophila melanogaster
• Alignment Length: 1741
• Identity: 498/1741 - (28%)
• Similarity: 761/1741 - (43%)
• Gaps: 405/1741 - (23%)

Human    21 VQFGVLSPDELKRMSVTE--GGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQ----TCAGNMT 79
            ::|.|.:..|::::||.:  .||.:...   |....|||.|.|.|   ..|||.    ||. .:.
  Fly    16 LEFAVFTDQEIRKLSVVKVITGITFDAL---GHAIPGGLYDIRMG---SYGRCMDPCGTCL-KLQ 73

Human    80 ECPGHFGHIELAKPVFHVGFLVKTMKVLRCV-CFFCSKLLVDSNNPKIKDILAKSKGQPKKRL-- 141
            :||||.|||||..||:: .|.:|.::.|.|: |..|.||.:..:..:|..:        :.||
  Fly    74 DCPGHMGHIELGTPVYN-PFFIKFVQRLLCIFCLHCYKLQMKDHECEIIML--------QLRLID 129

Human   142 ------THVYDLCKGKNICEGGEEMDNKFGVEQPEGD----------------EDLTKEKGHGGC 184
                  ....:|.|.:.:|:..|.:     |....||                |..:.......|
  Fly   130 AGYIIEAQELELFKSEIVCQNTENL-----VAIKNGDMVHPHIAAMYKLLEKNEKNSSNSTKTSC 189

Human   185 GRYQPRIRRSGLE-LYAEWKHVNEDSQ----EKKILLSPERVHEIFKRISD-------------E 231
            . .:..|..|.|: |..:.:|.|:..:    ..:.|:....:.:|.:|:..             :
  Fly   190 S-LRTAITHSALQRLGKKCRHCNKSMRFVRYMHRRLVFYVTLADIKERVGTGAETGGQNKVIFAD 253

Human   232 ECFVLGMEPRYAR------PEWMIVTV-------------------------LPVPPLSVRP--- 262
            ||      .||.|      ||.:.:.|                         |||.|...||
  Fly   254 EC------RRYLRQIYANYPELLKLLVPVLGLSNTDLTQGDRSPVDLFFMDTLPVTPPRARPLNM 312

Human   263 -AVVMQGSARNQDDL-------THKLADIVKI--NNQLRRNEQNGAAAHVIAEDV---KL----- 309
             ..:::|:.  |.|:       .|.|..::|.  ..|.:..|:..||...:..:.   ||
  Fly   313 VGDMLKGNP--QTDIYINIIENNHVLNVVLKYMKGGQEKLTEEAKAAYQTLKGETAHEKLYTAWL 375

Human   310 -LQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNL 373
             ||..|..::|   ..:.|.| |||   :.|||.::.|.|.:|.::|||||:::||||||||||:
  Fly   376 ALQMSVDVLLD---VNMSREM-KSG---EGLKQIIEKKSGLIRSHMMGKRVNYAARTVITPDPNI 433

Human   374 SIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNG-------DRIDLR- 430
            ::|::|:|...|..:::...||.:|:..|:::|..|...:|||.||...||       |....|
  Fly   434 NVDEIGIPDIFAKKLSYPVPVTEWNVTELRKMVMNGPDVHPGANYIQDKNGFTTYIPADNASKRE 498

Human   431 -----FHPKPSDLHLQTGYK-VERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPW-STFRLNLSV 488
                 ....|.|     |.| |.||:.:||:::.||||:|||.|:|||:.|||.. .||||:.|.
  Fly   499 SLAKLLLSNPKD-----GIKIVHRHVLNGDVLLLNRQPSLHKPSIMGHKARILHGEKTFRLHYSN 558

Human   489 TTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDV 553
            ...|||||||||||.|.|||...|||...|..|....:.|:...|:.|::||.:.:..|.:.|..
  Fly   559 CKAYNADFDGDEMNAHYPQSEVARAEAYNLVNVASNYLVPKDGTPLGGLIQDHVISGVKLSIRGR 623

Human   554 FLERGEVMNLL-MFLSTW--DGKVPQPAILKPRPLWTGKQIFSLIIPGHINCI-----RTH---- 606
            |..|.:...|: ..||..  |.|:..|.||||..||:||||.|.||   ||.|     |.:
  Fly   624 FFNREDYQQLVFQGLSQLKKDIKLLPPTILKPAVLWSGKQILSTII---INIIPEGYERINLDSF 685

Human   607 ------------------STHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLV 653
                              .|:|:..|.        .:::|.:.||||::|:|.|:..|.:...|:
  Fly   686 AKIAGKNWNVSRPRPPICGTNPEGNDL--------SESQVQIRNGELLVGVLDKQQYGATTYGLI 742

Human   654 HISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGD----SIAD---SKTYQDIQNTIKKAKQ 711
            |..|...|.|::.|..:....|...:|.:||.|:|:.|    .:||   .|..::.:|....|..
  Fly   743 HCMYELYGGDVSTLLLTAFTKVFTFFLQLEGFTLGVKDILVTDVADRKRRKIIRECRNVGNSAVA 807

Human   712 DVIEVIEKAHNNELEP------TPGNTLRQTFENQVNRILNDARDKTGSSA--QKSLSEY--NNF 766
            ..:|:.::..::||..      ...:..|...:.:...:|:...:...|:.  :..::::  ||.
  Fly   808 AALELEDEPPHDELVEKMEAAYVKDSKFRVLLDRKYKSLLDGYTNDINSTCLPRGLITKFPSNNL 872

Human   767 KSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGL 831
            :.||:||||||.:|..|:..::||..:||||.|.....::||.|...:..|:|.||::..::.|:
  Fly   873 QLMVLSGAKGSMVNTMQISCLLGQIELEGKRPPLMISGKSLPSFTSFETSPKSGGFIDGRFMTGI 937

Human   832 TPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL 896
            .|.:||||.|.||||||||||||:.:||:||.|||.:|.:.|.||.|||:|.|.|||..|||||
  Fly   938 QPQDFFFHCMAGREGLIDTAVKTSRSGYLQRCLIKHLEGLSVHYDLTVRDSDNSVVQFLYGEDG- 1001

Human   897 AGESVEFQNLATLKPSNKAFEKKFRFDY--TNERALRRTLQEDLVKDVLSNAHIQN--------- 950
                     |..||  :|.|..||..|:  .|..|:.|..|..|:||....|.:|.
  Fly  1002 ---------LDILK--SKFFNDKFCADFLTQNATAILRPAQLQLMKDEEQLAKVQRHEKHIRSWE 1055

Human   951 -----ELEREF----ERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHP 1006
                 :|...|    |.:||:.||.|                                 |::::.
  Fly  1056 KKKPAKLRAAFTHFSEELREEVEVKR---------------------------------PNEINS 1087

Human  1007 ----IKVVEGVKELSKKLVIVNGDD-----------PLSRQAQENATLLFNIHLRSTLCSRRMAE 1056
                .:..||:.:|.||   .:.:|           |....|.....|.:......|   |::..
  Fly  1088 KTGRRRFDEGLLKLWKK---ADAEDKALYRKKYARCPDPTVAVYKQDLYYGSVSERT---RKLIT 1146

Human  1057 EFRLSGEAFDWLLGEI-ESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLG 1120
            ::.....|....:.:| ..|..:::|.|||.||.:||||:|||:|||||||||:||....|||||
  Fly  1147 DYAKRKPALKETIADIMRVKTIKSLAAPGEPVGLIAAQSIGEPSTQMTLNTFHFAGRGEMNVTLG 1211

Human  1121 VPRLKELINI-SKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRK----VTANTAIYYDPN 1180
            :|||:|::.: |...||||:.:.:.......||:.:   ..|...||..    |..:|.:..||.
  Fly  1212 IPRLREILMLASSNIKTPSMDIPIKPGQQHQAEKLR---INLNSVTLANLLEYVHVSTGLTLDPE 1273

Human  1181 ------------PQSTVVAED----QEWVNVYYEMPDF--------DVARISPWLLRVELDRKHM 1221
                        |:. |..||    .:.:..|.....|        .|:..|.....|.:|.|..
  Fly  1274 RSYEYDMRFQFLPRE-VYKEDYGVRPKHIIKYMHQTFFKQLIRAILKVSNASRTTKIVVIDDKKD 1337

Human  1222 TDRK----LTMEQIAEKINAGFGDDLNCIFNDDN---AEKL---------------VLRIRIMNS 1264
            .|::    |.......:..|...||.:...|||:   ..||               |..:...|.
  Fly  1338 ADKEDDNDLDNGDEVGRSKAKANDDDSSDDNDDDDATGVKLKQRKTDEKDYDDPDDVEELHDAND 1402

Human  1265 DENKMQ---EEEEVVDKMDDDVFLRCIESNMLTDMT---------------------------LQ 1299
            |:::.:   :||:..|..|:|...:.:|..:..||.                           |.
  Fly  1403 DDDEAEDEDDEEKGQDGNDNDGDDKAVERLLSNDMVKAYTYDKENHLWCQVKLNLSVRYQKPDLT 1467

Human  1300 GI--EQISKVYMHLPQTDNKKKIII---TEDGEFKALQEWILETDGVSLMRVLS-EKDVDPVRTT 1358
            .|  |...|..:|  |..:.|:.||   |:|.:       :|:|||:::..:.. .|.:|..|..
  Fly  1468 SIIRELAGKSVVH--QVQHIKRAIIYKGTDDDQ-------LLKTDGINIGEMFQHNKILDLNRLY 1523

Human  1359 SNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQD 1423
            ||||..|....||||..:.:.:|:.:|....|..|:.|||:|:.|.||..|....::|.|: ...
  Fly  1524 SNDIHAIARTYGIEAASQVIVKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKGM-EHS 1587

Human  1424 TGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLL 1474
            :.||.:.|||.::..|..||..|.:|.:...|..:|:|.....|||.|:||
  Fly  1588 SSPLQQMSFESSLQFLKSAAGFGRADELSSPSSRLMVGLPVRNGTGAFELL 1638

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
POLR2A	NP_000928.1	RNAP_II_RPB1_N	19..876	CDD:259848	304/1018 (30%)
		Bridging helix. /evidence=ECO:0000250|UniProtKB:G3MZY8	832..873		26/40 (65%)
		RNA_pol_Rpb1_6	896..1079	CDD:461511	40/218 (18%)
		RNAP_II_Rpb1_C	1058..1476	CDD:132720	142/505 (28%)
		Trigger loop. /evidence=ECO:0000250|UniProtKB:G3MZY8	1083..1124		29/40 (73%)
		CTD	1491..1610	CDD:215026
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1546..1970
		C-terminal domain (CTD), 52 X 7 AA approximate tandem repeats of Y-[ST]-P-[STQ]-[ST]-P-[SRTEVKGN]	1593..1960
		Herpes_BLLF1	<1854..>1959	CDD:282904
Polr1A	NP_523743.1	RNAP_I_RPA1_N	18..982	CDD:259844	304/1016 (30%)
		HMG-box_SF	1065..1119	CDD:438789	14/89 (16%)
		RNAP_I_Rpa1_C	1166..1638	CDD:132722	138/485 (28%)