Protein Alignment POLR2A and Polr3A

DIOPT Version :10

• Sequence 1: NP_000928.1
• Gene: POLR2A / 5430
• HGNCID: 9187
• Length: 1970
• Species: Homo sapiens
• Sequence 2: NP_001096987.2
• Gene: Polr3A / 32524
• FlyBaseID: FBgn0030687
• Length: 1383
• Species: Drosophila melanogaster
• Alignment Length: 1550
• Identity: 510/1550 - (32%)
• Similarity: 759/1550 - (48%)
• Gaps: 289/1550 - (18%)

Human    16 RTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGR-PKLGGLMDPRQGVIERTGRCQTCAGNMT 79
            :.|..||||:...||:::.::..  |......:..| |...|::|.|.|:..:...|:||...:.
  Fly    13 KKISHVQFGISGADEIQQEALVR--IISKNLYQAQRQPVPYGVLDRRMGISTKDAMCETCGQGLN 75

Human    80 ECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPK-----K 139
            ||.||||:::||.||||:|....|:.:|:.:|..|:.:::   .|:.:.:..|....|.     |
  Fly    76 ECIGHFGYLDLALPVFHIGHFRSTINILQMICKVCAHVML---KPEDRQLYEKKLHNPNFSYLGK 137

Human   140 RLTHVYDLCKGKNICE-------------------------GGEEMDNKFG------VEQPEGDE 173
            :..||..|.|.|.:.:                         .|.:||:.|.      :...:.:.
  Fly   138 KALHVQMLAKAKKVTKCPHCGSPNGGVKKGPGLLKILHDPYKGRKMDSLFTSNMNEMLRSTQTNR 202

Human   174 DLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGM 238
            ||....|                         |..:.|:   |:|..|.::|::|...:..:|||
  Fly   203 DLNSTLG-------------------------NYSTAEE---LTPLMVLDLFEQIPQRDVALLGM 239

Human   239 EPRYARPEWMIVTVLPVPPLSVRPAVVMQGSA-RNQDDLTHKLADIVKINNQLRRNEQNGAAAHV 302
            ....|.|:.:|||.:.|||..:||:|:.:..| ..:||||.|.::|:.||:.::|:...|....:
  Fly   240 CSHDAHPKHLIVTRVFVPPACIRPSVLSEVKAGTTEDDLTMKQSEILLINDVIQRHMATGGKIEL 304

Human   303 IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVI 367
            |.||...||.|||....:|:.|:|..|... :..:.:.||||||:||.|.||.|||||||.||||
  Fly   305 IHEDWDFLQLHVALYFHSEISGIPINMAPK-KTTRGIVQRLKGKQGRFRCNLSGKRVDFSGRTVI 368

Human   368 TPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGA-----------KYIIR 421
            :|||||.|:|||||..:|..:|:.|.|.|.||..::||||.|.|.:|||           ||:..
  Fly   369 SPDPNLMINQVGVPVRVAKILTYPERVNPANIRHMRELVRNGPSMHPGANYVQQRGSSFKKYLAY 433

Human   422 DNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNL 486
            .|.:::         ...|:.|..||||:.|||||:|||||:|||||:|.||.::.|..|||.|.
  Fly   434 GNREKV---------AQELKCGDVVERHLRDGDIVLFNRQPSLHKMSIMCHRAKVQPQRTFRFNE 489

Human   487 SVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKR 551
            ...||||||||||||||||||:.|.|||...|......:|||::...::...||.:|.....|::
  Fly   490 CACTPYNADFDGDEMNLHLPQTEEARAEALILMGNQSNLVTPKNGEILIAATQDFITGGYLLTQK 554

Human   552 DVFLERGEVMNL-LMFLSTWDG----KVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPD 611
            :|||.:.|.|.| ..||:..|.    |:|.||:||||.||||||:|||::            .|:
  Fly   555 EVFLTKEEAMQLAACFLANEDSTMHIKLPPPALLKPRRLWTGKQMFSLLM------------RPN 607

Human   612 DEDSGPYKHISPG------------DTKVVVENGELIMGILCKKSLGTSAGSLVHISYL---EMG 661
            |:.......::.|            |:.:.:.|.||:.|::.|.::|  :|:...|.||   :.|
  Fly   608 DDSQVRLNMVNKGRNYTRNKDLCSNDSWIHIRNSELMCGVMDKATMG--SGTKQCIFYLLLRDFG 670

Human   662 HDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELE 726
            ..........:..:.:.:|...|.:.||.|.....|..|..:..:........|.||......|:
  Fly   671 ESHATKAMWRLARIASYFLQNRGFSFGISDVTPSKKLLQHKELLLNNGYAKCNEYIELLKAGTLQ 735

Human   727 PTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQ 791
            ..||.|..:|.|:.:.|.|:..|::...:....|...|:...|.:||:|||.|||||:||.||||
  Fly   736 CQPGCTPEETLESVMLRELSAIREQAAKTCFAELHPTNSALIMALSGSKGSNINISQMIACVGQQ 800

Human   792 NVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAE 856
            .:.|||:|.||::|.||||.:....|.:||||:||:.:||||||||||.|.|||||:||||||||
  Fly   801 AISGKRVPNGFENRALPHFERHSAIPAARGFVQNSFYSGLTPTEFFFHTMAGREGLVDTAVKTAE 865

Human   857 TGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFR 921
            |||:||||:|.:|.::|.||.||||::|::|...||.|||...|:|.:|    ||.:...:
  Fly   866 TGYLQRRLVKCLEDLVVHYDGTVRNAVNEMVDTIYGGDGLDPVSMETRN----KPVDLVHQ---- 922

Human   922 FDYTNERALRRTLQEDLVKDVLSNAHIQNE-LER-----EFERMREDREVLRVIFPTGDSKVVLP 980
              |.|.||     |....||...||...:| ||.     ||...|:|
  Fly   923 --YDNLRA-----QHPQGKDRPLNAEEMSEALETLLRTPEFAEARDD------------------ 962

Human   981 CNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHL 1045
                         |.::.|           ..:..:||::      ..|.::.::...|...|..
  Fly   963 -------------FKLDVR-----------NHINTVSKRI------GQLQKRYEKCIDLCHQIEC 997

Human  1046 RSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYA 1110
            .:|              |.....:..|..::|:|:..||..|||:||||:|||.|||||.|||:|
  Fly   998 LTT--------------EQLLQFVRRINDRYNRAVTEPGTAVGAIAAQSIGEPGTQMTLKTFHFA 1048

Human  1111 GVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAI 1175
            ||::.|:|.||||:.|:||.:|...||.:|..|  ::....|.|:.:..|:|.|||.::::...:
  Fly  1049 GVASMNITQGVPRIVEIINATKTISTPIITAEL--ENCHSMEFARQVKARIEKTTLAELSSYVEV 1111

Human  1176 YYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELD-------RKHMTDRKLTMEQIAE 1233
            ..  .|.|..:|     :.|       |:|||....|.::||       :..|..:...:|.:|.
  Fly  1112 VC--GPYSCYLA-----IGV-------DMARIKLLGLHIDLDTIVFSILKSRMRVKPTQVEVVAS 1162

Human  1234 KINAGFGDDLNCIFNDDNAEKLVLRIR-----IMNSDENKMQEEEEVVDKMDDDVFLRCIESNML 1293
            :                  .::|:|:.     .:|::..::.
  Fly  1163 Q------------------SRIVVRVEATRTSTINAELARLA----------------------- 1186

Human  1294 TDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTT 1358
              ::||.:     |...||..:  :.:|..:|.......:..:|  |..|..|::...|...||.
  Fly  1187 --LSLQNV-----VVAGLPNIN--RAVIAVDDARQPPTYKLCIE--GYGLRDVIATYGVVGKRTR 1240

Human  1359 SNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQD 1423
            ||:|.||:..|||||.|..:..|:..|:...|..|::||:.||...||.||.::.|||||:.:..
  Fly  1241 SNNICEIYQTLGIEAARTIIMSEITEVMEGHGMSVDWRHIMLLASQMTARGEVLGITRHGLAKMR 1305

Human  1424 TGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAE 1478
            .......|||:|.|.|.:||.:|::|.:.||||.|:||..|..|||.|.||...|
  Fly  1306 ESVFNLASFEKTADHLFDAAYYGQTDAINGVSERIILGMPACIGTGIFKLLQQHE 1360

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
POLR2A	NP_000928.1	RNAP_II_RPB1_N	19..876	CDD:259848	335/925 (36%)
		Bridging helix. /evidence=ECO:0000250|UniProtKB:G3MZY8	832..873		31/40 (78%)
		RNA_pol_Rpb1_6	896..1079	CDD:461511	33/188 (18%)
		RNAP_II_Rpb1_C	1058..1476	CDD:132720	131/429 (31%)
		Trigger loop. /evidence=ECO:0000250|UniProtKB:G3MZY8	1083..1124		28/40 (70%)
		CTD	1491..1610	CDD:215026
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1546..1970
		C-terminal domain (CTD), 52 X 7 AA approximate tandem repeats of Y-[ST]-P-[STQ]-[ST]-P-[SRTEVKGN]	1593..1960
		Herpes_BLLF1	<1854..>1959	CDD:282904
Polr3A	NP_001096987.2	RNAP_III_RPC1_N	24..889	CDD:259847	334/921 (36%)
		RNAP_III_Rpc1_C	1013..1353	CDD:132723	126/407 (31%)