Protein Alignment Kat60 and SPAST

DIOPT Version :10

• Sequence 1: NP_001262276.1
• Gene: Kat60 / 53566
• FlyBaseID: FBgn0040208
• Length: 605
• Species: Drosophila melanogaster
• Sequence 2: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Alignment Length: 414
• Identity: 154/414 - (37%)
• Similarity: 235/414 - (56%)
• Gaps: 35/414 - (8%)

  Fly   197 AAPNNRRTTPATQNSRPSSTIPQSTARNGPASTRNSRNSTSATAPSGGARTTNGRAGGRKLSTSN 261
            |.|..:.....|.||.|.|.....|...| .|..:...|.|..:...|.:..:|.|......|..
Human   231 AVPKRKDPLTHTSNSLPRSKTVMKTGSAG-LSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPK 294

  Fly   262 TNEARDDDSTAAGNNGGAAGDGENGDPQAAQEEERKFQPNNHIEAELVDILERDILQKDPKVRWS 326
            ||......:                 |..|..:::..:...::::.|.:::..:|:.....|::.
Human   295 TNRTNKPST-----------------PTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFD 342

  Fly   327 DIADLHDAKRLLEEAVVLPMLMPDYFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVS 391
            |||....||:.|:|.|:||.|.|:.|.|:|.|.:|:|:.||||.||||||||||.|...||||:|
Human   343 DIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNIS 407

  Fly   392 SATLTSKYRGESEKMVRLLFEMARFYAPSTIFIDEIDS-LCSRRGSESEHEASRRVKSELLVQMD 455
            :|:|||||.||.||:||.||.:||...||.|||||:|| ||.||  |.||:||||:|:|.|::.|
Human   408 AASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERR--EGEHDASRRLKTEFLIEFD 470

  Fly   456 GV-GGGEEQAKVVMVLAATNFPWDIDEALRRRLEKRIYIPLPSDEGREALLKINL--REVKVDDS 517
            || ..|:::   |:|:.|||.|.::|||:.||..||:|:.||::|.|..||| ||  ::......
Human   471 GVQSAGDDR---VLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLK-NLLCKQGSPLTQ 531

  Fly   518 VDLTYVANELKGYSGADITNVCREASMMSMRRKIAGLTPEQIRQLATEEV-DLPVSNKDFNEAMS 581
            .:|..:|....||||:|:|.:.::|::..:|.    |.|||::.::..|: ::.:|  ||.|::.
Human   532 KELAQLARMTDGYSGSDLTALAKDAALGPIRE----LKPEQVKNMSASEMRNIRLS--DFTESLK 590

  Fly   582 RCNKSVSRADLDKYEKWMREFGSS 605
            :..:|||...|:.|.:|.::||.:
Human   591 KIKRSVSPQTLEAYIRWNKDFGDT 614

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Kat60	NP_001262276.1	Kp60-NTD	62..129	CDD:439297
		PHA03291	<167..>253	CDD:223033	14/55 (25%)
		SpoVK	219..566	CDD:440232	134/350 (38%)
		RecA-like_KTNA1	327..493	CDD:410930	94/167 (56%)
		Vps4_C	<570..603	CDD:462762	10/32 (31%)
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		18/69 (26%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200
		MIT_spastin	116..195	CDD:239142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266		10/35 (29%)
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		154/414 (37%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		10/74 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		7/50 (14%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	94/167 (56%)
		AAA_lid_3	533..587	CDD:465537	18/59 (31%)
		Vps4_C	<580..612	CDD:462762	10/33 (30%)