Protein Alignment Myo28B1 and MYO4

DIOPT Version :10

• Sequence 1: NP_723294.1
• Gene: Myo28B1 / 53515
• FlyBaseID: FBgn0040299
• Length: 2122
• Species: Drosophila melanogaster
• Sequence 2: NP_009373.1
• Gene: MYO4 / 851204
• SGDID: S000000027
• Length: 1471
• Species: Saccharomyces cerevisiae
• Alignment Length: 1649
• Identity: 438/1649 - (26%)
• Similarity: 692/1649 - (41%)
• Gaps: 403/1649 - (24%)

  Fly     7 PRKGEYVWVKPQNTTSEF---------AVPFGARIVRTEKTQTLVCDDRNKQFWVPAGDVLKAMH 62
            |.| |..|:..:.|.::|         .:..|..:  :.:|.:...||.:     |...||:...
Yeast    12 PHK-EQGWIGGEVTKNDFFEGTFHLELKLEDGETV--SIETNSFENDDDH-----PTLPVLRNPP 68

  Fly    63 ITSQEDVEDMITLGDLQEYTILRNLQNRYAKQLIYTYTGSMLVAINPY-QILPIYTNREIQLYRN 126
            |.  |..:|:.||..|.|..:|..::.||....||||:|.:|:|.||: ::..:|:...||.|.:
Yeast    69 IL--ESTDDLTTLSYLNEPAVLHAIKKRYMNGQIYTYSGIVLIAANPFDKVDHLYSREMIQNYSS 131

  Fly   127 KSLAELPPHIFAISDNAFQRLQRLKENQCVVISGESGAGKTESTKLILQYLAAISGKH------- 184
            |...||.||:|||::.|::.:...|.||.||:|||||||||.|.|.|::|.|::...:
Yeast   132 KRKDELEPHLFAIAEEAYRFMVHEKANQTVVVSGESGAGKTVSAKYIMRYFASVQESNNREGEVE 196

  Fly   185 -SWIEQQIIEANPIMEAFGNAKTVRNDNSSRFGKYIEIRFTPQGAIQGARIQQYLLEKSRIVFQS 248
             |.||.||:..||||||||||||.|||||||||||::|.|.....|:|::|:.|||||||:|:|.
Yeast   197 MSQIESQILATNPIMEAFGNAKTTRNDNSSRFGKYLQILFDENTTIRGSKIRTYLLEKSRLVYQP 261

  Fly   249 RDERNYHIFYCMLAGLSTAERERLKLQEQSPSQYHYLAQGGCFTLPGRGDAKDFADIRAAMKVLS 313
            ..||||||||.:|.||....::.|.|  .||..|||..|||...:.|..:|:::.....|:.::.
Yeast   262 ETERNYHIFYQILEGLPEPVKQELHL--SSPKDYHYTNQGGQPNIAGIDEAREYKITTDALSLVG 324

  Fly   314 FKPEEVWSILSLLAAILHLGNLRFTAT-EVANLATAEIDDTPNLQRVAQLLGIPISALNAALTQR 377
            ...|....|..:||.:||:||:....| ..|:|::.|    .|||...:||||........:.::
Yeast   325 INHETQLGIFKILAGLLHIGNIEMKMTRNDASLSSEE----QNLQIACELLGIDPFNFAKWIVKK 385

  Fly   378 TIFVHGEHVTTSLSKEAAIEGRDAFVKSLYDGIFVRIVRRINETIN----KQVDQPMNSIGVLDI 438
            .|....|.:.|:|:...|:..||:..|.:|..:|..:|..||:|:.    .|.|...:.||:|||
Yeast   386 QIVTRSEKIVTNLNYNQALIARDSVAKFIYSTLFDWLVDNINKTLYDPELDQQDHVFSFIGILDI 450

  Fly   439 FGFENFDNNSFEQLCINYANENLQQFFVGHIFKMEQDEYQNEHINWQHIEFQDNQQILDLIGMKP 503
            :|||:|:.|||||.|||||||.|||.|..|:||:||:||..|.|.|..|||.|||..:|||..|
Yeast   451 YGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFSDNQPCIDLIENK- 514

  Fly   504 MNLMSLIDEESKFPKGTDQTLLEKLHVQHG---NRSIYVKGKTTQTSLFGIRHYAGVVMYNPLGF 565
            :.::||:||||:.|.|:|::...||:....   :..::.|.:..||. |.:.|||..|.|...||
Yeast   515 LGILSLLDEESRLPSGSDESWASKLYSAFNKPPSNEVFSKPRFGQTK-FIVSHYAVDVEYEVEGF 578

  Fly   566 LEKNRDSFSGDLRTLVQRSTNKYLVDIF----------PHE--------MPMDTAKKQPTLCVKF 612
            :||||||.|.....:.:.:||.....|.          |.|        :|...::|:|||...|
Yeast   579 IEKNRDSVSLGHLDVFKATTNPIFKQILDNRELRSDDAPEEQNTEKKIMIPARLSQKKPTLGSMF 643

  Fly   613 RNSLDMLMRTLSQAHPYFIRCIKPNEYKEPKNFDKELCVRQLRYSGMMETARIRRAGYPIRHAYR 677
            :.||..||..::..:.::|||||||..|:|..||..:.:.|||..|::||.||..||:|.|..:.
Yeast   644 KKSLGELMAIINSTNVHYIRCIKPNSEKKPWEFDNLMVLSQLRACGVLETIRISCAGFPSRWTFD 708

  Fly   678 AFVERYRLLVPP---VGPLEQCDCRKLA-----RQICEVALPADSDRQYGKTKLFLRDEDDASLE 734
            .||:||.||...   .|.|...|..|.|     :.|.:..:...:..|.|.||:|.:....|.||
Yeast   709 EFVQRYFLLTDYSLWSGILYNPDLPKEAIVNFCQSILDATISDSAKYQIGNTKIFFKAGMLAFLE 773

  Fly   735 LQRSQLMLKSIVTIQRGIRRVLFRRYMKRYREAIITVQRYWRGRLQRRKYQ-------------- 785
            ..|:..|.:..:.||:.||...:|....:..|:|...|...|..|.|.:..
Yeast   774 KLRTNKMNEICIIIQKKIRARYYRLQYLQTMESIKKCQSQIRSLLVRTRVDHELKTRAAILLQTN 838

  Fly   786 ---VMRQGFHR--LGACIAAQ----------QLTTKFTMVRC----------------RTIK--- 816
               :.::.::|  :|..|..|          .:..||.::..                ||:|
Yeast   839 IRALWKREYYRAAIGQIIKLQCTCKRKLILDSVNRKFMLMAAVIIQSYIRSYGHKTDYRTLKRSS 903

  Fly   817 --LQALSRGYLVRKDF---QKKLLER-----------RKQNQLKKEELLKLAKMKEAEELLRLQQ 865
              :|:..|..|.|:.:   ||::.||           .:..:.|...:|.|..:.::  ..||.|
Yeast   904 ILVQSAMRMQLARRRYIVLQKEVEERNIRASYGIGLLEEAIEFKNSFILNLEMLNDS--YTRLTQ 966

  Fly   866 LKE-------QKEREQRE------------------------QQEKRLQEEQRLKAEAAARNALA 899
            |.:       .|:|::.|                        .::..|:|.::.::.....:..:
Yeast   967 LLQGDLSNIPSKQRQEYETIVNGYNDKISKLKTLQVEIMNTLNKKNALKERKKKQSSLIQSHMQS 1031

  Fly   900 MAAVQQKR------RTKPVKQEAPKAPTLQARNSLPPPPTTLIVA-----------APLPTRPAS 947
            :||::..:      ..|.:|||......:.|::.     ||...|           |....:|
Yeast  1032 LAAIKGNKPSRLSDEVKSMKQELAFIENVIAQDF-----TTTYSANKNDKVKGLGIAGQQVKP-- 1089

  Fly   948 AVTRINTIPESPGTIDVESSKQMVDDVFRFLNDEPDAALRKLNNIS-SGDTIRLPKSVPNNIDTS 1011
              ..:|.|....|..|:   .:::.|:..:..:..:..|:|:|... :||.:..|..|...:
Yeast  1090 --KLVNVIRRESGNPDL---LELLMDLNCYTLEVTEGYLKKVNVTEVNGDNVLGPIHVITTV--- 1146

  Fly  1012 DFSYLKYAATYFGGGATAQHERKPLKKSLLKHEH-----PIDE-MASKAIWLTILRFM------- 1063
                   .::....|...| ..|.:.|.||..|.     |.|| |.....||:.|..:
Yeast  1147 -------VSSLVRNGLLIQ-SSKFISKVLLTVESIVMSLPKDETMLGGIFWLSNLSRLPAFAANQ 1203

  Fly  1064 --------GDLPDVVSSPTLHVFDNENLMSDLASLLNTSDSYKPRLFVRQSQRRIPKPLASGEKE 1120
                    ||..|.::...|:..:||.|                                   |.
Yeast  1204 KTLYEANGGDEKDKLTLIYLNDLENETL-----------------------------------KV 1233

  Fly  1121 AQEFYQHWL----NVPTSHLEKIHFIIGHGIIKNSLRDEILAQICKQLYLNPSRSSYSRGWLLLS 1181
            ..:.|..||    ...::|:|....::...:.||| .||..|::.  .:||
Yeast  1234 FDKIYSTWLVKFMKHASAHIEIFDMVLNEKLFKNS-GDEKFAKLF--TFLN-------------- 1281

  Fly  1182 LCLSCFPPSKEFEPHLRSFMKQGTAQLQATPSLQRLERTLVNGPRCQPPSLFELHAIRGRHPLRL 1246
                      ||:..|..|
Yeast  1282 ----------EFDAVLCKF---------------------------------------------- 1290

  Fly  1247 DIHLMDGQQRRLQVDAASTAREAVNQLCQGMGLTDTFGFGLVMSLNGKLMPLGAGQEHVLDAISE 1311
              .::|....::                    ..||..:..||..|              |.|::
Yeast  1291 --QVVDSMHTKI--------------------FNDTLKYLNVMLFN--------------DLITK 1319

  Fly  1312 CEQRQLDAPWKLYIRKEMFATWYDPSMDPKATQLIYKQILNGLKCGEYRCRSEKDIAMVCALACF 1376
            |........:::....|...:|::|.::.....||  ||:..:|..:.:..:..:..::  ...:
Yeast  1320 CPALNWKYGYEVDRNIERLVSWFEPRIEDVRPNLI--QIIQAVKILQLKISNLNEFKLL--FDFW 1380

  Fly  1377 VEYGPGEI----LRLKP-SEITAFVPSDLL-------------APGERAIENWSRLIAA----TY 1419
            ....|.:|    |:.|| ::..|.||:::|             .||:..|...::..:|    .|
Yeast  1381 YALNPAQIQAILLKYKPANKGEAGVPNEILNYLANVIKRENLSLPGKMEIMLSAQFDSAKNHLRY 1445

  Fly  1420 EKSSYVKEEQNDLLLEAQKRAKED 1443
            :.|:..:....:.|....|..|.|
Yeast  1446 DTSAITQNSNTEGLATVSKIIKLD 1469

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Myo28B1	NP_723294.1	MYSc_Myo7	81..726	CDD:276832	274/687 (40%)
		IQ	765..785	CDD:197470	6/19 (32%)
		Atrophin-1	<820..>946	CDD:460830	30/187 (16%)
		MyTH4	1031..1239	CDD:470587	37/232 (16%)
		FERM1_F1_Myosin-VII	1243..1334	CDD:340612	10/90 (11%)
		B41	1248..1429	CDD:214604	32/202 (16%)
		PH-like	1454..1547	CDD:473070
		MyTH4	1674..1819	CDD:214535
		FERM2_F1_Myosin-VII	1825..1919	CDD:340613
		B41	1827..2032	CDD:214604
		FERM_C2_MyoVII	2028..2121	CDD:270020
MYO4	NP_009373.1	COG5022	2..1471	CDD:227355	438/1649 (27%)