DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Myo28B1 and hum-4

DIOPT Version :9

Sequence 1:	NP_723294.1	Gene:	Myo28B1 / 53515	FlyBaseID:	FBgn0040299	Length:	2122	Species:	Drosophila melanogaster
Sequence 2:	NP_001359540.1	Gene:	hum-4 / 181335	WormBaseID:	WBGene00002037	Length:	2577	Species:	Caenorhabditis elegans

Alignment Length:	2721	Identity:	550/2721 - (20%)
Similarity:	961/2721 - (35%)	Gaps:	832/2721 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly    69 VEDMITLGDLQEYTILRNLQNRYAKQLIYTYTGSMLVAINPYQILPIYTNREIQLYRNKSLAELP 133
            ::::|.|.|..|..|.:||..|:.|.:.||...::||.:|.:.    ..:.|.||....|...
Worm     1 MDNLIELPDQSEAGIAQNLHERFKKGVTYTKASNVLVFVNDFN----DKDSEDQLSWETSSTS-- 59

  Fly   134 PHIFAISDNAFQRLQRLKEN-QCVVISGESGAGKTESTKLILQYLAA-----ISGKHSWIEQQII 192
             .:.|::.||..::..:..| :.:|..||||:||:.:.....:||.:     :|.|||    ..|
Worm    60 -GVNAVAKNALNKIFNMSSNAESIVFGGESGSGKSYNVFKAFKYLTSQPKSKVSTKHS----SSI 119

  Fly   193 EANPIMEAFGNAKTVRNDNSSRFGKYIEIRFTPQGAIQGARIQQYL-LEKSRIVFQSRDERNYHI 256
            |.  :.::||.|||::||.::|||..|::.: .:..:.|..::..: ||..|::.|...|||::|
Worm   120 EF--VFKSFGCAKTLKNDEATRFGCSIDLLY-KRNVLTGLNLKYTVPLEVPRVISQKPGERNFNI 181

  Fly   257 FYCMLAGLSTAERERLKLQEQSPSQYHYLAQGGCFTLPGRGDAKDFADIRAAMKVLSFKPEEVWS 321
            ||.:..|||  :..:.|...:...::.|:.||.. :...:.|...|..:.:|:.||.|..:...|
Worm   182 FYEVYHGLS--DEMKAKFGIKGLQKFFYINQGNS-SENIQHDVNRFKHLESALHVLGFSDDHCMS 243

  Fly   322 ILSLLAAILHLGNLRFTATEVANLA--TAEIDDTPNLQRVAQLLGIPISALNAALTQRTIFVHGE 384
            |..:::.|||:||:.|......|:.  ..||.:...|:.:|.||.:....|     .:.:....|
Worm   244 IYKIISTILHIGNIYFRTKRNPNVEQDVVEIGNLAELKWIAFLLEVDFDQL-----VKFLLPTSE 303

  Fly   385 HVTTSLSKEAAIEGRDAFVKSLYDGIFVRIVRRINETINKQVDQPMNSIGVLDIFGFENFDNNSF 449
            ..:| :...||::.||:|...:|:.:|..::.||.  :..:.......|.:||.:|||.::||..
Worm   304 DGST-IELNAALDNRDSFAMMIYEELFKWVLNRIG--LQLKCSLHTGVISILDHYGFEKYNNNGV 365

  Fly   450 EQLCINYANENLQQFFVGHIFKMEQDEYQNEHINWQHIEFQ-----DNQQILDLIGMKPMNLMSL 509
            |:..||..||.::..||.|.|..:..:|..:.|:   ::::     :|.:.::|:..||..|:||
Worm   366 EEFLINSVNERIENLFVKHCFHDQLIDYAKDGIS---VDYKVPNSIENGKTVELLFKKPYGLLSL 427

  Fly   510 IDEESKFPKGTDQTLLEKLHVQHGNRSIYVKGKTTQTSLFGIRHYAGVVMYNPLGFLEKNRDSFS 574
            :.:|.||||||.:|.||..::.|.:||.|.|.:..:...||:||..|...||...|..:|:...|
Worm   428 LTDECKFPKGTHETYLEHCNLNHTDRSAYGKARNKERLEFGVRHCIGTTWYNVTDFFARNKRIIS 492

  Fly   575 GDLRTLVQRSTNKYLVDIFP----HEMPMDTAKKQPTLCVKFRNSLDMLMRTLSQAHPYFIRCIK 635
            .....|::.|.|..:..:|.    :...:..::.|..|    |.:.|:..: ::.:|.:|:||||
Worm   493 LSAVQLMRNSKNPIIGLLFESYGGNTSDIIVSQAQFVL----RGAQDIADK-INVSHVHFVRCIK 552

  Fly   636 PNEYKEPKNFDKELCVRQLRYSGMMETARIRRAGYPIRHAYRAFVERYRLLVPPVGPLEQCDCRK 700
            .|..::...||..|..||::...:.|....|..|||::.:...|..:||.|:|  |.:.||...|
Worm   553 SNNERQSTKFDIPLVNRQIKNLLLAELLSFRVKGYPVKISKTTFARQYRCLLP--GDIAQCQNEK 615

  Fly   701 -LARQICE-VALPADSDRQYGKTKLFLRDEDDASLELQRSQLMLKSIVTIQRGIRRVLFRRYMKR 763
             :.:.|.: ..:..:.|.:.|...:|||:......:..::::...:.:.||:.::..:.::..||
Worm   616 EIIQDILQGQGVKYEDDFKIGTEYVFLRERLAERYDGLQNKICGDAAIIIQKNMKSFVAQKVYKR 680

  Fly   764 YREAIITVQRYWRGRLQRRKYQVMRQ-----------------GFHR-LGACIAAQQLTT----- 805
            .|.|||.:|...||...||.|.:.|:                 .:|: ||...:.|...|
Worm   681 MRAAIIKLQSGLRGWKARRDYIIKREEMFKAIGRTMKRNKRLDAYHQALGTENSGQLQQTLVGYI 745

  Fly   806 -------KF------------TMVRCRTI-------KLQALSRGYLVRKDFQKKLLERRKQ---- 840
                   ||            |:....|:       .:.:::......::|:..|||.|::
Worm   746 DINEDAKKFLERPTSDSDATETLTHYLTVPMKNFLSNMNSITLEQFAEENFKGHLLEPRREPIMT 810

  Fly   841 NQLKKE---------ELLKL---------AKMKEAEELLRL---QQLKEQKEREQ---------R 875
            ..|.||         |:.||         ...|:.|:|.|.   |.:....:|::         .
Worm   811 PFLHKESDYDFRLSVEIFKLILKYMNDIKLTKKQREDLGRYIVQQGISNPCQRDEILVQTINQIN 875

  Fly   876 EQQEKRLQE------EQRLKAEAAARNALAMAAVQQKRRTKPVKQEAPKAPTLQAR--------- 925
            :.|:|...:      ...:.......|.:.|......:.:.|:|::.  ..|||.|
Worm   876 KNQDKTASDNGWKLVHMAISVFPPTENIIPMLIGFFNKESVPMKEQL--FATLQRRLKIYDSEIA 938

  Fly   926 NSLPPPPTTLIVAAPLPTRPASAVTRIN------------------------------------T 954
            ..|||....||....:|    :.|..||                                    |
Worm   939 RELPPSNLELIATPNIP----NTVAEINCYDGLCCDVHLSPWSTTSEIAERILKQRGVGIALGWT 999

  Fly   955 IP-ESPGTIDVESSKQMVDDVFR---------------FLNDEPDAALRK-----------LNNI 992
            :. |:|..:...:....:.|||.               |.|..|:..::|           ...:
Worm  1000 VEVETPNRVFAPTGNHFIHDVFSQIEGANMDSENQQNIFFNFPPEKLVKKPPVVPKEVVQEQEIV 1064

  Fly   993 SSGDTIRLPKSV-------------PNNIDT-------------SDFSYLKYAATYFGGGATAQH 1031
            |......:||:.             |...::             ||.|.::......|...|...
Worm  1065 SQNTNGTIPKAARTYPTPEEWINQPPQRTESRNTPRDHQKPDEESDESLVREYNEEAGYAYTLPR 1129

  Fly  1032 ERKPLKKSL-----LKHEHPIDEMASKAIWLTI-----LRFMGDLPD---VVSSPTL-------- 1075
            :.||..:::     .:.|...::.|::..:.|:     ::.|..:.|   ::.||.|
Worm  1130 KNKPAPQAVEDNETSESEEDDEDAANRVGYETVRPEENMKMMETIQDHSHILKSPVLPRKTYSRN 1194

  Fly  1076 ----------------HVFDNENLMSDLASLLNTSD----------------------------- 1095
                            ..:..:..|.....::.||.
Worm  1195 EQHEEYTPMNFAPPPTFTYPQQMPMMQYVPVMMTSSMMTPSMMTPSMMPGQQIAMVPQQMMMQPH 1259

  Fly  1096 -SYKPRLFVRQSQRRIPKPLASGEKEAQEFYQHWLNVPTSHLEKIHFIIGHG--------IIK-- 1149
             ||.|: :.:..|...|:|:.|.:....|       ||......:|  .|||        |:|
Worm  1260 FSYVPQ-YPQIPQYCPPEPILSPQSVRSE-------VPPMMAPVMH--DGHGSTRSRDYRIMKRG 1314

  Fly  1150 -------------------------NSLRDEILA------------QICKQL---------YLNP 1168
                                     :::.|::|:            |:...:         |..|
Worm  1315 EVPSQYSTIRNMPVPEHGKDVDQFLDAVFDQVLSKDEQRAAHFNSNQLANTIKGGKLRPEGYYEP 1379

  Fly  1169 ----------------------------------------------SRSSYS------------- 1174
                                                          ||:|||
Worm  1380 PVQTYSPVPPRYPTLRRVDDSPLRSRAKSLPRIISPRHEHFVRRPHSRNSYSNESTSSDDQMNYR 1444

  Fly  1175 --------------RGW---------------------LLLSLCLS------------------- 1185
                          .|:                     :|||...|
Worm  1445 TRSRERSLPRFHSNNGYNYDPSQPVYMMPVQMNGHGEMILLSPVASEHKAQSRHRTHDRSHRHHV 1509

  Fly  1186 -----------------CFPPSKE-----------FEPHL-RSFMKQGTAQLQATPSL------- 1214
                             ..||...           ..||| :|.:.:.|::.:...:|
Worm  1510 PSGVERYMAKRSPSVDILKPPMSRRTPDAMVEQTYVRPHLAQSPVGRQTSRFEEFSALPRGDSRA 1574

  Fly  1215 -QRLERTLV-----------NGPRCQPP------------------------SLFELHAIRGR-- 1241
             :|:|..|:           ||....||                        ..:...|::..
Worm  1575 RERMENPLLARQIPRAYPYQNGNVVPPPPSSYRAPSPAPTSGDRRGLNRLPQESYTEPAVKNTKN 1639

  Fly  1242 -------HPLRLDIHLMDGQQRRLQVDAASTAREAVNQLCQGMGLTDTF--------GFGLVMSL 1291
                   |...||      :|:.:......||:.|:|.      |:|..        ...|:..:
Worm  1640 NSEYLSPHRFNLD------EQKAVIRHEKETAKNAINM------LSDRLRKLPPPVDNVRLIRPI 1692

  Fly  1292 NGKLMPLGAG----------------------------QEHVL------DAISECEQRQ------ 1316
            .....|:...                            :|.|:      |.:::...|.
Worm  1693 TPAQPPITQSPVPSEPVVVRTPSPVPQPTPPPPPPPVREEWVIRDSVERDPVTQGRIRGSFRKEP 1757

  Fly  1317 -------------------LDAPWKLYIRKEMFATWYDPSM--DPKATQLIYKQILNGLKCG-EY 1359
                               :.|||||.||||||   |...:  |.:....::.||:...|.. .|
Worm  1758 LVPQPPRPPVVAEKPAVKFVKAPWKLTIRKEMF---YPGEVLNDIQIIDQVFAQIVEDCKKSYPY 1819

  Fly  1360 RCRSE--KDIAMVCALACFVEYGPGEILRLKPSEITAFVPSDLLAPGERAIENWSRLIAATYEKS 1422
            |.|.:  |.:..|                |:..||.   ||||        .|.|.:
Worm  1820 RIREQDRKQVETV----------------LRQYEIP---PSDL--------NNQSNI-------- 1849

  Fly  1423 SYVKEEQNDLLLEAQKRAKEDICLFAHLSWPMRHSRLFEVVRKEGPKLQSDELMLGINSAGLFLI 1487
                          ....|..:...|.| ||:..::::|||.|...  :|...:..|:..|:.||
Worm  1850 --------------HPDVKVAVIELARL-WPLYFNQVYEVVEKRPD--ESVSTIFAISEHGIRLI 1897

  Fly  1488 DETEQVL-----------------ASCCFSEVLKVHVESDDKLHVMTFQHVNFVLQCSSAQDANE 1535
            ..|...|                 .|...:::|.|||..:|:.:..:...:    :.:.|....:
Worm  1898 VHTPHDLENPLKIQDFFPFETIADVSLEANDILSVHVRHEDEENAYSAVRI----KTNQAPQIKK 1958

  Fly  1536 VINYMLD-NLRQRSSYGVALDPVVEGDLEDCLVLNPGDLIEFEAGVTGAQLMAGNAQDCYRGCVN 1599
            .::..|. .:..:..:..||:..|..::.. |....||:||......|.....||   ...|.:.
Worm  1959 TLDRCLSGGVVPKRKFVRALEDYVTSEVNH-LSFKQGDVIELLQEPEGETPPVGN---WLYGKIE 2019

  Fly  1600 GQWGQFLAGNVRVLATLTKP---SEKLQDILREGRFQEPPKPTPRANYSRRRQHNISQLAESHFR 1661
            .::|..||..|........|   .|..:|.....||.:...|     :|..| :.:...|..:||
Worm  2020 NRFGFLLAQYVDSTDGDNVPPIRHETSEDRDERVRFFDDEVP-----FSSER-YTMIDFATKYFR 2078

  Fly  1662 EPLDSDK---------APLSKFSPEPLKAPLLKAVVKVPPLFQQALVMHHHILKYMGDIARSNLP 1717
            :|.|..|         :.:.:||.:|:...||..:......:  |:...|.|:|:|||.......
Worm  2079 KPKDKKKQETWAWEDISQIVRFSEKPISQSLLADLGNEESKY--AVETFHAIMKFMGDEPLKKSE 2141

  Fly  1718 VNTDLIFQPAL---QHPLLCDELYCQLMKQLSDNPSSE--SEKRGWDLLYLATGLVAPSVLVMRE 1777
            ..||::|:..|   :.|.|.||:||||:||.:.|.|.:  |..|.|.||.:.|... ||.|.::.
Worm  2142 SMTDVVFKVLLICHRQPTLRDEVYCQLIKQTTSNISQKPNSALRAWRLLTIITAYF-PSSLTLKP 2205

  Fly  1778 LII-LLRMRAD-------ALADACLKRLKRSLAQGQRKKAPHLIEVEGIQQRCLHIYHKIYFPDD 1834
            .:: .|...||       ..|..|...:.::...|.||...:.:||:.|...|.......|...|
Worm  2206 YVLQYLGDNADEWQRPFHGTARICQTNMIQTFKYGGRKVLLNALEVQQITDGCQLRRQAFYISKD 2270

  Fly  1835 TVEAFEIESHTRGAELIADIAQRLELKS---PVGYSIFLKTG--DRVYAMPEEEFVFDFITQLIY 1894
            ...:..:...|...|:|.::...|.::|   ...:|:....|  ..:.....:.::.|.||:
Worm  2271 HNVSQTLRPITVAEEMIQELCNLLNVRSLHEQQEFSLCYTVGKDKHLNYCKNDNYLMDIITE--- 2332

  Fly  1895 WLRQQRTIRSISDGQYQLHFMRKLWLNNHPGEDLNGDMIFS-YPQELHKYLKGYYPIDCEQASRL 1958
              .:.:.:      .:|.:..|.:|:  ||....|...|.| :.|.:..||:|    .....:.|
Worm  2333 --SEHKKL------PFQFYLKRTVWV--HPLRYDNAAYIDSMFDQVIDDYLRG----SLISTNSL 2383

  Fly  1959 AILVYSADHDVSLQRLPEVLTRLIPEDLIPLQTVAEWRQQILPK-----VHRDHLTE-------- 2010
            ..|..:...::.  :|...|..|:|::  |....|:...||:||     .|| |..|
Worm  2384 GQLTAATTEEII--KLAAYLFLLLPDN--PKGLNAKTLPQIVPKSVIEPKHR-HQEEMVTRISRQ 2443

  Fly  2011 ----------DHAKILFLQELSHFACFGSTFFVVKQ--QNDDALPETLLIAINSTGFHMLDPTTK 2063
                      ..||..||:.||.:..||...:.:|.  :|...|||.:| .||.:|..:|.|.:|
Worm  2444 LKMFGGRMRPAEAKSHFLELLSTWPTFGVLHYRLKSVVENGHQLPEVIL-TINKSGIQLLQPKSK 2507

  Fly  2064 EILRSYEYSQL-GIWSSGKNHFHI---RFGNMIGASKLLCSTTQGYKMDDLLASYV 2115
            |:.:...|.|: .:.|..|..:.|   ....|.|...|...|.:..::..|:..|:
Worm  2508 EVFKERNYDQIVSVESIRKTAYKIVRLVINTMQGEETLDIKTDEADEISHLIGQYM 2563

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Myo28B1	NP_723294.1	MYSc	62..737	CDD:214580	190/687 (28%)
		MYSc_Myo7	81..726	CDD:276832	184/664 (28%)
		IQ	765..785	CDD:197470	9/19 (47%)
		DUF4355	827..>887	CDD:290964	19/99 (19%)
		MyTH4	1031..1239	CDD:295320	60/515 (12%)
		B41	1248..1429	CDD:214604	43/252 (17%)
		FERM_N	1248..>1288	CDD:286467	7/47 (15%)
		FERM_B-lobe	1342..1429	CDD:271216	17/89 (19%)
		PH-like	1454..1547	CDD:302622	19/110 (17%)
		MyTH4	1674..1819	CDD:214535	47/157 (30%)
		B41	1827..2032	CDD:214604	48/233 (21%)
		FERM_N	1829..>1877	CDD:286467	9/52 (17%)
		FERM_M	1936..2032	CDD:278785	28/118 (24%)
		FERM_C2_MyoVII	2028..2121	CDD:270020	25/94 (27%)
hum-4	NP_001359540.1	MYSc_Myo12	13..643	CDD:276841	184/664 (28%)
		IQ	680..701	CDD:197470	10/20 (50%)
		MyTH4	803..952	CDD:214535	28/150 (19%)
		PRK10263	<1162..>1357	CDD:236669	26/204 (13%)
		SH3	1974..2030	CDD:388381	15/59 (25%)
		MyTH4	2100..2255	CDD:214535	47/157 (30%)
		B41	2262..2475	CDD:214604	48/234 (21%)
		PH-like	2489..2563	CDD:388408	19/74 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910