Protein Alignment Myo28B1 and hum-2

DIOPT Version :10

• Sequence 1: NP_723294.1
• Gene: Myo28B1 / 53515
• FlyBaseID: FBgn0040299
• Length: 2122
• Species: Drosophila melanogaster
• Sequence 2: NP_001317742.1
• Gene: hum-2 / 179321
• WormBaseID: WBGene00002036
• Length: 1811
• Species: Caenorhabditis elegans
• Alignment Length: 2031
• Identity: 538/2031 - (26%)
• Similarity: 833/2031 - (41%)
• Gaps: 517/2031 - (25%)

  Fly     1 MDAGYSPRKGE-------------------YVWVKPQNTTSEFAVPFGARIVRTEKTQTLVCDDR 46
            ||.|.:..:|:                   .||:   ..|.|..:.|..|.||..     :.||.
 Worm     1 MDGGATLGRGQLFPLQNFKKGVRIWHRHPTLVWI---GATLEEDITFQTRNVRIR-----LEDDT 57

  Fly    47 NKQFWVPAGDVLKAMH----ITSQEDVEDMITLGDLQEYTILRNLQNRYAK-QLIYTYTGSMLVA 106
            ..::.:.:.|.|..:.    :..:   :|:..|..|.|..:|.|||.|:.| ..||||.|.:|||
 Worm    58 EVEYAIKSLDQLPFLRNPAFLVGK---DDLTLLSYLHEPAVLHNLQVRFVKGSSIYTYCGIVLVA 119

  Fly   107 INPY-QILPIYTNREIQLYR--NKSLAELPPHIFAISDNAFQRLQRLKENQCVVISGESGAGKTE 168
            |||| ....||....||:||  .||..|:.|||||:::.|...:....::|.:::|||||||||.
 Worm   120 INPYADCSHIYGEEIIQVYRGAGKSAREMDPHIFAVAEEAHFDMGAFGKSQSIIVSGESGAGKTV 184

  Fly   169 STKLILQYLAAI------SGKHSWIEQQIIEANPIMEAFGNAKTVRNDNSSRFGKYIEIRFTPQG 227
            |.|.:::|||::      :|..:.||.:::.:|||||:.|||||:||||||||||:|:|.|..:|
 Worm   185 SAKFVMRYLASVAASKTRNGGTTSIEARVLASNPIMESIGNAKTIRNDNSSRFGKFIQINFCERG 249

  Fly   228 -AIQGARIQQYLLEKSRIVFQSRDERNYHIFYCMLAGLSTAERERLKLQEQSPSQ-YHYLAQGGC 290
             .|.||.::.|||||||:|||:..||||||||.:.|   ....:.||.....|.: |.||.|||.
 Worm   250 RRIVGAEMKTYLLEKSRLVFQAPGERNYHIFYQLCA---ARNHQVLKDLHLGPCESYSYLTQGGD 311

  Fly   291 FTLPGRGDAKDFADIRAAMKVLSFKPEEVWSILSLLAAILHLGNLRFTATEVANLATAEIDDTPN 355
            ..:||..|..||..:..|:::|.|..:::..:..|||.:|.|||:.|...|.::..:|.     :
 Worm   312 SRIPGVDDKADFEALLKALQLLGFDEKQMSDVFRLLAGLLLLGNVHFENGEGSSAVSAS-----S 371

  Fly   356 LQRVAQLL----GIPISALNAALTQRTIFVHGEHVTTSLSKEAAIEGRDAFVKSLYDGIFVRIVR 416
            .|.:::|.    .|..|.|...||:|.|....|.||..|:|..|:..|||..|.||..:|..:|.
 Worm   372 CQEISRLCREFWKISESDLRIWLTRREIRAVNEIVTKPLTKNEAVRSRDALTKMLYSHLFGWLVD 436

  Fly   417 RINETINK--------QVDQPMNSIGVLDIFGFENFDNNSFEQLCINYANENLQQFFVGHIFKME 473
            :|||.:|:        |..:|...||||||:|||.||.|||||..||||||.|||.|..|:||:|
 Worm   437 KINEALNEKDKLDGTNQKKRPDRFIGVLDIYGFETFDVNSFEQFSINYANEKLQQQFNQHVFKLE 501

  Fly   474 QDEYQNEHINWQHIEFQDNQQILDLIGMKPMNLMSLIDEESKFPKGTDQTLLEKL----HVQHGN 534
            |:||..|.|.|..::|.|||..:||| ..|:.:::|:||:.|...|:|...|.:|    .::...
 Worm   502 QEEYIREEIEWVRVDFHDNQPAIDLI-EGPVGMINLLDEQCKRLNGSDADWLSQLQNSTELKRNP 565

  Fly   535 RSIYVKGKTTQTSLFGIRHYAGVVMYNPLGFLEKNRDSFSGDLRTLVQRSTNKYLVDIFPHEMPM 599
            :..:.|.::..   |.:||:|..|.|:..||:|||||:....|..:|..|...::..:.....|.
 Worm   566 QLAFPKVRSND---FIVRHFAADVTYSTDGFVEKNRDAIGEQLLDVVVASKFPFIRTVIGSTAPT 627

  Fly   600 D-------------TAKKQPTLCVKFRNSLDMLMRTLSQAHPYFIRCIKPNEYKEPKNFDKELCV 651
            .             |.||  |:..:||:||..||..|....|:::||||||:.|...:|:.:..:
 Worm   628 SVSSSSSSSTPGKRTIKK--TVASQFRDSLKELMSVLCSTRPHYVRCIKPNDSKISFDFEPKRAI 690

  Fly   652 RQLRYSGMMETARIRRAGYPIRHAYRAFVERYRLLVPPVGPLEQCDCRKLARQICEVALPADSDR 716
            :|||..|::||.||..||:|.|:.|..|..|||::......|.:...::.|...|:..|   .:.
 Worm   691 QQLRACGVLETVRISAAGFPSRYPYEEFARRYRVIYTKEAALWRDKPKQFAELACQQCL---EEG 752

  Fly   717 QY--GKTKLFLRDEDDASLE-----------------------LQRSQLMLKSIVTIQRGIRRVL 756
            :|  ||||:|||....|.||                       .::.:.|.:|::.:|..::..|
 Worm   753 KYAVGKTKIFLRTGQVAVLERVRLDTLAAAATVIQKMWKGFLARRKYETMRRSLLIVQASLKAFL 817

  Fly   757 -FRR--YMKRYREAIITVQRYWRGRLQRRKYQVMRQGFHRLGACIAAQQLTTKFTMVRCR--TIK 816
             |||  |::.:| |:|.:|...||.|:||||:.:|.....:.|...|.::......:|..  .|.
 Worm   818 AFRRIKYLQMHR-AVIVMQSAVRGYLERRKYEQIRDSIIGIQAMFKANRVRRYVEKLRYEKSAIT 881

  Fly   817 LQALSRGYLVRKD-------------FQKKLLERRKQNQLKKE-----ELLKL-----AKMKEAE 858
            :||..||||.|::             ..:|.|.:|:..:||.|     .|.||     .|:.|.:
 Worm   882 IQAAWRGYLARREQIANRKKVVMVQCAVRKWLAKRRLRELKIEARSVGHLQKLNTGLENKIIELQ 946

  Fly   859 ELLRLQQLKEQKEREQREQQEKRLQEEQRLKAEAAARNALAMAAVQQKRRTKPVKQEAPKAPT-- 921
            ..|.:...:.::|.|:.....|.|   |:.||:.|...|..:..::.:.|.:.:::|..:..|
 Worm   947 MRLDIANARTKEEAEKFATASKNL---QKTKADLAMMEAERLTLLEARNRVEVLQEEVERLETEC 1008

  Fly   922 --LQARNSLPPPPTTLIVAAPLPTRPASAVTRINTI---PESPGTIDVESSKQM----VDDVFRF 977
              .:|:.            ..:.|:.....:|::..   .||..|| ||.::|:    .|   |.
 Worm  1009 DLKEAQR------------GGMETKMVELQSRLDQFQMQSESGQTI-VELTEQLEKAKAD---RV 1057

  Fly   978 LNDEP----DAAL---RKLNNISSGDTIRLPKSVPNNID-------------------------T 1010
            |.||.    :|||   |...|....:...:.:.:..|:|                         |
 Worm  1058 LWDEERQRMEAALNTERSARNALDAEMAAMREQLMKNVDLFESSTFQKRPSQKKNRDDDSCSRTT 1122

  Fly  1011 SDFSYLK---YAATYFGGGATAQHERKPLKKSLLKHEHPIDEMASKAIWLTILRFMGDLPD---- 1068
            |:.|.|.   .|.|..|..:|::...:.|          :|.|||.   ...||.:.||..
 Worm  1123 SNLSQLTGSFTAETINGVHSTSRGSPEVL----------LDNMAST---FEQLRMINDLRQRNEH 1174

  Fly  1069 ----------VVSSPT----------LHVFDN----------ENLMSDLASLLNTSDSYKPRLFV 1103
                      ::.:.|          |..|::          ..|.:|:..|::..:.....:|.
 Worm  1175 CQRETERMKAIIEASTLIETLDKKTSLKAFESIRVGELEGAYNRLKNDMERLVSGENGATHSVFE 1239

  Fly  1104 R--QSQRRIPKPLASGEKEAQEFYQHWLNVPTSHLEKIHFIIGHGIIKNSLRDEILAQICKQLYL 1166
            |  :...|:       .:||.|.    .::.:||.|| ..:.|....:.|.|.:  :..|...
 Worm  1240 RIMEENERL-------REEAVEL----RSMLSSHFEK-QSVAGSSGYRRSPRPD--SGHCSGA-- 1288

  Fly  1167 NPSRSSYSRGWLLLSLCL--SCFPPSKEFEPHLRSFMKQGTAQL-QATPSLQRLE--------RT 1220
             .|....|...|...||:  .|        .||::..:..|..| .....::||:        :|
 Worm  1289 -DSEDGSSGADLEEDLCIERQC--------RHLKNLAENLTKMLTNQNLEIERLQQQLRFSESQT 1344

  Fly  1221 LVNGPRCQPPSLFELHAIRGRHPLRLDIHLMDGQQRRLQVDAASTAREAVNQLCQGMGLTDTFGF 1285
            :.....|   ||.|  |:||.|.   ...|:..|...|.......:.|......|
 Worm  1345 VFRPSDC---SLDE--AVRGAHK---QTQLLAQQNMDLNDKLTRQSEELAEARAQ---------- 1391

  Fly  1286 GLVMSLNGKLMPLGAGQEHVLDAISECEQRQLDAPWKLYIRKEMFATWYDP---------SMDPK 1341
                 |.|...|||      |:..|:.|..:|:|..|..|:...|...|:.         .:.|.
 Worm  1392 -----LRGYSGPLG------LENASDEEIIRLEAFEKGSIKHSGFLEVYNVPEFARIIVCELKPT 1445

  Fly  1342 ATQLIYKQILNGLKCGEYRCRSEK-DIAMVCALACFVEYGPGEILRLKPSEITAFVPSDLLA--- 1402
            ..:|:.|.:...|....:|...|| |...:..|...|.      |.||.: |:.....|||:
 Worm  1446 LARLLTKNLPAYLLVAAFRNHDEKRDETALTGLFSSVH------LVLKDT-ISRSHDLDLLSLWL 1503

  Fly  1403 -------------PGERAIENWSRLIAATYEKSSYVKEEQNDLLLEAQKRAKEDICLFAHLSWPM 1454
                         .||.:...|.  :|.|        |.||....:|...|            |:
 Worm  1504 VNLWRLFNLLRQYSGEDSQPEWH--VANT--------ETQNSYRFKAYDVA------------PI 1546

  Fly  1455 RHSRLFEVVRKEGPKLQSDELMLGINSAGLFLIDETEQVLASCCFSEVLKVHVESDDKLHVMTFQ 1519
            |                 |:|.|.|..                |::.::|..:|     ||::.:
 Worm  1547 R-----------------DQLKLRIEE----------------CYTSLMKKAIE-----HVLSPK 1573

  Fly  1520 HVNFVLQCSSAQD---ANEVINYMLDNLRQRSSYGV-----ALDPVVEGDLEDCLVLNPGDLIEF 1576
            .|..:||..|:.|   |.:       ..|.|:|..|     :||                ||::|
 Worm  1574 IVPGILQHESSSDLMTAGQ-------ERRDRNSGSVESQRKSLD----------------DLLQF 1615

  Fly  1577 EAGVTGAQLMAGNAQDCYRGCVNGQ---WGQFLAGNVRVLATLTKPSEKLQDILREGRFQEPPKP 1638
             ..:...:|......|.....|.||   |...||.|..:........||...|
 Worm  1616 -MEIVHTKLTTYGGDDIVVKQVIGQMARWMCALALNYMMFRRELCNFEKAIQI------------ 1667

  Fly  1639 TPRANYSRRRQHNISQL-----------AESHFREPLDSDKAPLSKFSPEPLKAPLLKAVVKVPP 1692
                      :||::|:           ...||...:.:.....|:..|..|.....:...::.|
 Worm  1668 ----------KHNVTQIQNWLNAKGLSDCRDHFEPLVQACHLLQSRKDPSNLDTLCGEMTSRLKP 1722

  Fly  1693 LFQQALVMHHHILKYMGDIARSNLPVNTDLIFQPALQHPLLCDELYCQLMKQLSD------NPSS 1751
            ....|::.|:.....|.|                     .|..|...|:.|:|::      :|..
 Worm  1723 RQVVAILQHYDPSDEMED---------------------GLSPEFLVQIQKKLNERAIANNDPIE 1766

  Fly  1752 ESEKRGWDLLYLATGL 1767
            :.:|    |:.|.|.|
 Worm  1767 DKDK----LIMLGTYL 1778

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Myo28B1	NP_723294.1	MYSc_Myo7	81..726	CDD:276832	270/687 (39%)
		IQ	765..785	CDD:197470	10/19 (53%)
		Atrophin-1	<820..>946	CDD:460830	31/152 (20%)
		MyTH4	1031..1239	CDD:470587	47/254 (19%)
		FERM1_F1_Myosin-VII	1243..1334	CDD:340612	18/90 (20%)
		B41	1248..1429	CDD:214604	42/206 (20%)
		PH-like	1454..1547	CDD:473070	17/95 (18%)
		MyTH4	1674..1819	CDD:214535	18/100 (18%)
		FERM2_F1_Myosin-VII	1825..1919	CDD:340613
		B41	1827..2032	CDD:214604
		FERM_C2_MyoVII	2028..2121	CDD:270020
hum-2	NP_001317742.1	MYSc_Myo5	93..764	CDD:276831	270/687 (39%)
		IQ	779..799	CDD:197470	0/19 (0%)
		IQ	826..847	CDD:197470	9/21 (43%)
		CCDC158	<864..1473	CDD:464943	140/694 (20%)
		Myo5_CBD	1429..1803	CDD:271254	90/488 (18%)